Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay.
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Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay. / Müller, V; Thomssen, C; Karakas, C; Eustermann, I; Ramirez Porras, J; Coith, C; Berger, J; Löning, Thomas; Jänicke, F; Pantel, K.
In: INT J BIOL MARKER, Vol. 18, No. 1, 1, 2003, p. 13-20.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay.
AU - Müller, V
AU - Thomssen, C
AU - Karakas, C
AU - Eustermann, I
AU - Ramirez Porras, J
AU - Coith, C
AU - Berger, J
AU - Löning, Thomas
AU - Jänicke, F
AU - Pantel, K
PY - 2003
Y1 - 2003
N2 - PURPOSE: The HER-2/neu protein (p185) has become a promising target for antibody therapy in breast cancer. We tested the feasibility of a quantitative approach for HER-2/neu testing based on the analysis of tumor tissue extracts by an enzyme-linked immunosorbent assay (ELISA). EXPERIMENTAL DESIGN: Tumor tissue extracts of primary human breast cancers (n=124) were prepared using a triton-based buffer. HER-2/neu concentration was quantified by ELISA. Paraffin-embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER-2/neu antibody TAB 250 (n=124) and by chromogenic in situ hybridization (CISH) (n=73). RESULTS: Concentrations of p185 in tissue extracts determined by ELISA varied from 1 to 927 ng per mg protein with a median of 25 ng/mg protein, whereas normal breast tissue showed values from 0.4 to 5.5 ng/mg with a median of 2.2 ng/mg (p
AB - PURPOSE: The HER-2/neu protein (p185) has become a promising target for antibody therapy in breast cancer. We tested the feasibility of a quantitative approach for HER-2/neu testing based on the analysis of tumor tissue extracts by an enzyme-linked immunosorbent assay (ELISA). EXPERIMENTAL DESIGN: Tumor tissue extracts of primary human breast cancers (n=124) were prepared using a triton-based buffer. HER-2/neu concentration was quantified by ELISA. Paraffin-embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER-2/neu antibody TAB 250 (n=124) and by chromogenic in situ hybridization (CISH) (n=73). RESULTS: Concentrations of p185 in tissue extracts determined by ELISA varied from 1 to 927 ng per mg protein with a median of 25 ng/mg protein, whereas normal breast tissue showed values from 0.4 to 5.5 ng/mg with a median of 2.2 ng/mg (p
M3 - SCORING: Zeitschriftenaufsatz
VL - 18
SP - 13
EP - 20
JO - INT J BIOL MARKER
JF - INT J BIOL MARKER
SN - 0393-6155
IS - 1
M1 - 1
ER -