PortalPublikationenQuantitative assessment of HER-2/neu protein concentration i...

Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay.

Standard

Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay. / Müller, V; Thomssen, C; Karakas, C; Eustermann, I; Ramirez Porras, J; Coith, C; Berger, J; Löning, Thomas; Jänicke, F; Pantel, K.

in: INT J BIOL MARKER, Jahrgang 18, Nr. 1, 1, 2003, S. 13-20.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Müller, V, Thomssen, C, Karakas, C, Eustermann, I, Ramirez Porras, J, Coith, C, Berger, J, Löning, T, Jänicke, F & Pantel, K 2003, 'Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay.', INT J BIOL MARKER, Jg. 18, Nr. 1, 1, S. 13-20. <http://www.ncbi.nlm.nih.gov/pubmed/12699058?dopt=Citation>

APA

Müller, V., Thomssen, C., Karakas, C., Eustermann, I., Ramirez Porras, J., Coith, C., Berger, J., Löning, T., Jänicke, F., & Pantel, K. (2003). Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay. INT J BIOL MARKER, 18(1), 13-20. [1]. http://www.ncbi.nlm.nih.gov/pubmed/12699058?dopt=Citation

Vancouver

Müller V, Thomssen C, Karakas C, Eustermann I, Ramirez Porras J, Coith C et al. Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay. INT J BIOL MARKER. 2003;18(1):13-20. 1.

Bibtex

@article{abc35efb461b47d7882d2f42c14b4275,
title = "Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay.",
abstract = "PURPOSE: The HER-2/neu protein (p185) has become a promising target for antibody therapy in breast cancer. We tested the feasibility of a quantitative approach for HER-2/neu testing based on the analysis of tumor tissue extracts by an enzyme-linked immunosorbent assay (ELISA). EXPERIMENTAL DESIGN: Tumor tissue extracts of primary human breast cancers (n=124) were prepared using a triton-based buffer. HER-2/neu concentration was quantified by ELISA. Paraffin-embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER-2/neu antibody TAB 250 (n=124) and by chromogenic in situ hybridization (CISH) (n=73). RESULTS: Concentrations of p185 in tissue extracts determined by ELISA varied from 1 to 927 ng per mg protein with a median of 25 ng/mg protein, whereas normal breast tissue showed values from 0.4 to 5.5 ng/mg with a median of 2.2 ng/mg (p",
author = "V M{\"u}ller and C Thomssen and C Karakas and I Eustermann and {Ramirez Porras}, J and C Coith and J Berger and Thomas L{\"o}ning and F J{\"a}nicke and K Pantel",
year = "2003",
language = "Deutsch",
volume = "18",
pages = "13--20",
journal = "INT J BIOL MARKER",
issn = "0393-6155",
publisher = "Wichtig Publishing",
number = "1",

}

RIS

TY - JOUR

T1 - Quantitative assessment of HER-2/neu protein concentration in breast cancer by enzyme-linked immunosorbent assay.

AU - Müller, V

AU - Thomssen, C

AU - Karakas, C

AU - Eustermann, I

AU - Ramirez Porras, J

AU - Coith, C

AU - Berger, J

AU - Löning, Thomas

AU - Jänicke, F

AU - Pantel, K

PY - 2003

Y1 - 2003

N2 - PURPOSE: The HER-2/neu protein (p185) has become a promising target for antibody therapy in breast cancer. We tested the feasibility of a quantitative approach for HER-2/neu testing based on the analysis of tumor tissue extracts by an enzyme-linked immunosorbent assay (ELISA). EXPERIMENTAL DESIGN: Tumor tissue extracts of primary human breast cancers (n=124) were prepared using a triton-based buffer. HER-2/neu concentration was quantified by ELISA. Paraffin-embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER-2/neu antibody TAB 250 (n=124) and by chromogenic in situ hybridization (CISH) (n=73). RESULTS: Concentrations of p185 in tissue extracts determined by ELISA varied from 1 to 927 ng per mg protein with a median of 25 ng/mg protein, whereas normal breast tissue showed values from 0.4 to 5.5 ng/mg with a median of 2.2 ng/mg (p

AB - PURPOSE: The HER-2/neu protein (p185) has become a promising target for antibody therapy in breast cancer. We tested the feasibility of a quantitative approach for HER-2/neu testing based on the analysis of tumor tissue extracts by an enzyme-linked immunosorbent assay (ELISA). EXPERIMENTAL DESIGN: Tumor tissue extracts of primary human breast cancers (n=124) were prepared using a triton-based buffer. HER-2/neu concentration was quantified by ELISA. Paraffin-embedded tissue sections of the same tumors were analyzed by immunohistochemical staining applying the monoclonal HER-2/neu antibody TAB 250 (n=124) and by chromogenic in situ hybridization (CISH) (n=73). RESULTS: Concentrations of p185 in tissue extracts determined by ELISA varied from 1 to 927 ng per mg protein with a median of 25 ng/mg protein, whereas normal breast tissue showed values from 0.4 to 5.5 ng/mg with a median of 2.2 ng/mg (p

M3 - SCORING: Zeitschriftenaufsatz

VL - 18

SP - 13

EP - 20

JO - INT J BIOL MARKER

JF - INT J BIOL MARKER

SN - 0393-6155

IS - 1

M1 - 1

ER -