Protocol for generating lung and liver metastasis in mice using models that bypass intravasation
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Protocol for generating lung and liver metastasis in mice using models that bypass intravasation. / Lücke, Jöran; Zhang, Tao; Zazara, Dimitra E; Seeger, Philipp; Izbicki, Jacob R; Hackert, Thilo; Huber, Samuel; Giannou, Anastasios D.
In: STAR PROTOC, Vol. 5, No. 1, 15.03.2024, p. 102696.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Protocol for generating lung and liver metastasis in mice using models that bypass intravasation
AU - Lücke, Jöran
AU - Zhang, Tao
AU - Zazara, Dimitra E
AU - Seeger, Philipp
AU - Izbicki, Jacob R
AU - Hackert, Thilo
AU - Huber, Samuel
AU - Giannou, Anastasios D
N1 - Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2024/3/15
Y1 - 2024/3/15
N2 - Forced metastasis models, those in which the step of intravasation is bypassed, can be used to investigate the mechanisms underlying metastasis and evaluate potential therapeutic targets. Here, we present a protocol for using three forced models of lung and liver metastasis to generate metastasis within 3-4 weeks in approximately 99% of injected mice. We describe steps for cancer cell preparation, mouse analgesia and anesthesia; injecting through intrasplenic, intraportal, and intravenous techniques; and daily evaluation of metastasis. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.
AB - Forced metastasis models, those in which the step of intravasation is bypassed, can be used to investigate the mechanisms underlying metastasis and evaluate potential therapeutic targets. Here, we present a protocol for using three forced models of lung and liver metastasis to generate metastasis within 3-4 weeks in approximately 99% of injected mice. We describe steps for cancer cell preparation, mouse analgesia and anesthesia; injecting through intrasplenic, intraportal, and intravenous techniques; and daily evaluation of metastasis. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.
U2 - 10.1016/j.xpro.2023.102696
DO - 10.1016/j.xpro.2023.102696
M3 - SCORING: Journal article
C2 - 38244200
VL - 5
SP - 102696
JO - STAR PROTOC
JF - STAR PROTOC
SN - 2666-1667
IS - 1
ER -