Protocol for generating lung and liver metastasis in mice using models that bypass intravasation

Jöran Lücke; Tao Zhang; Dimitra E Zazara; Philipp Seeger; Jacob R Izbicki; Thilo Hackert; Samuel Huber; Anastasios D Giannou

doi:10.1016/j.xpro.2023.102696

Protocol for generating lung and liver metastasis in mice using models that bypass intravasation

Standard

Protocol for generating lung and liver metastasis in mice using models that bypass intravasation. / Lücke, Jöran; Zhang, Tao; Zazara, Dimitra E ; Seeger, Philipp; Izbicki, Jacob R; Hackert, Thilo ; Huber, Samuel ; Giannou, Anastasios D.

in: STAR PROTOC, Jahrgang 5, Nr. 1, 15.03.2024, S. 102696.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Lücke, J, Zhang, T, Zazara, DE , Seeger, P, Izbicki, JR, Hackert, T , Huber, S & Giannou, AD 2024, 'Protocol for generating lung and liver metastasis in mice using models that bypass intravasation', STAR PROTOC, Jg. 5, Nr. 1, S. 102696. https://doi.org/10.1016/j.xpro.2023.102696

APA

Lücke, J., Zhang, T., Zazara, D. E., Seeger, P., Izbicki, J. R., Hackert, T., Huber, S., & Giannou, A. D. (2024). Protocol for generating lung and liver metastasis in mice using models that bypass intravasation. STAR PROTOC, 5(1), 102696. https://doi.org/10.1016/j.xpro.2023.102696

Vancouver

Lücke J, Zhang T, Zazara DE , Seeger P, Izbicki JR, Hackert T et al. Protocol for generating lung and liver metastasis in mice using models that bypass intravasation. STAR PROTOC. 2024 Mär 15;5(1):102696. https://doi.org/10.1016/j.xpro.2023.102696

Bibtex

@article{58ba06c8665743768b0e6204df6c91bb,

title = "Protocol for generating lung and liver metastasis in mice using models that bypass intravasation",

abstract = "Forced metastasis models, those in which the step of intravasation is bypassed, can be used to investigate the mechanisms underlying metastasis and evaluate potential therapeutic targets. Here, we present a protocol for using three forced models of lung and liver metastasis to generate metastasis within 3-4 weeks in approximately 99% of injected mice. We describe steps for cancer cell preparation, mouse analgesia and anesthesia; injecting through intrasplenic, intraportal, and intravenous techniques; and daily evaluation of metastasis. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.",

author = "J{\"o}ran L{\"u}cke and Tao Zhang and Zazara, {Dimitra E} and Philipp Seeger and Izbicki, {Jacob R} and Thilo Hackert and Samuel Huber and Giannou, {Anastasios D}",

year = "2024",

month = mar,

day = "15",

doi = "10.1016/j.xpro.2023.102696",

language = "English",

volume = "5",

pages = "102696",

journal = "STAR PROTOC",

issn = "2666-1667",

publisher = "Cell Press",

number = "1",

}

RIS

TY - JOUR

T1 - Protocol for generating lung and liver metastasis in mice using models that bypass intravasation

AU - Lücke, Jöran

AU - Zhang, Tao

AU - Zazara, Dimitra E

AU - Seeger, Philipp

AU - Izbicki, Jacob R

AU - Hackert, Thilo

AU - Huber, Samuel

AU - Giannou, Anastasios D

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Forced metastasis models, those in which the step of intravasation is bypassed, can be used to investigate the mechanisms underlying metastasis and evaluate potential therapeutic targets. Here, we present a protocol for using three forced models of lung and liver metastasis to generate metastasis within 3-4 weeks in approximately 99% of injected mice. We describe steps for cancer cell preparation, mouse analgesia and anesthesia; injecting through intrasplenic, intraportal, and intravenous techniques; and daily evaluation of metastasis. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.

AB - Forced metastasis models, those in which the step of intravasation is bypassed, can be used to investigate the mechanisms underlying metastasis and evaluate potential therapeutic targets. Here, we present a protocol for using three forced models of lung and liver metastasis to generate metastasis within 3-4 weeks in approximately 99% of injected mice. We describe steps for cancer cell preparation, mouse analgesia and anesthesia; injecting through intrasplenic, intraportal, and intravenous techniques; and daily evaluation of metastasis. For complete details on the use and execution of this protocol, please refer to Giannou et al.1.

U2 - 10.1016/j.xpro.2023.102696

DO - 10.1016/j.xpro.2023.102696

M3 - SCORING: Journal article

C2 - 38244200

VL - 5

SP - 102696

JO - STAR PROTOC

JF - STAR PROTOC

SN - 2666-1667

IS - 1

ER -