Profiling of humoral immune responses to norovirus in children across Europe
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Profiling of humoral immune responses to norovirus in children across Europe. / Villabruna, Nele; Izquierdo-Lara, Ray W; Schapendonk, Claudia M E; de Bruin, Erwin; Chandler, Felicity; Thao, Tran Thi Nhu; Westerhuis, Brenda M; van Beek, Janko; Sigfrid, Louise; Giaquinto, Carlo; Goossens, Herman; Bielicki, Julia A; Kohns Vasconcelos, Malte; Fraaij, Pieter L A; Koopmans, Marion P G; de Graaf, Miranda.
In: SCI REP-UK, Vol. 12, No. 1, 14275, 22.08.2022.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Profiling of humoral immune responses to norovirus in children across Europe
AU - Villabruna, Nele
AU - Izquierdo-Lara, Ray W
AU - Schapendonk, Claudia M E
AU - de Bruin, Erwin
AU - Chandler, Felicity
AU - Thao, Tran Thi Nhu
AU - Westerhuis, Brenda M
AU - van Beek, Janko
AU - Sigfrid, Louise
AU - Giaquinto, Carlo
AU - Goossens, Herman
AU - Bielicki, Julia A
AU - Kohns Vasconcelos, Malte
AU - Fraaij, Pieter L A
AU - Koopmans, Marion P G
AU - de Graaf, Miranda
N1 - © 2022. The Author(s).
PY - 2022/8/22
Y1 - 2022/8/22
N2 - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.
AB - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.
KW - Caliciviridae Infections/immunology
KW - Capsid Proteins/genetics
KW - Child, Preschool
KW - Europe
KW - Gastroenteritis/immunology
KW - Genotype
KW - Humans
KW - Immunity, Humoral
KW - Infant
KW - Norovirus/genetics
KW - Phylogeny
U2 - 10.1038/s41598-022-18383-6
DO - 10.1038/s41598-022-18383-6
M3 - SCORING: Journal article
C2 - 35995986
VL - 12
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
M1 - 14275
ER -