Profiling of humoral immune responses to norovirus in children across Europe

Nele Villabruna; Ray W Izquierdo-Lara; Claudia M E Schapendonk; Erwin de Bruin; Felicity Chandler; Tran Thi Nhu Thao; Brenda M Westerhuis; Janko van Beek; Louise Sigfrid; Carlo Giaquinto; Herman Goossens; Julia A Bielicki; Malte Kohns Vasconcelos; Pieter L A Fraaij; Marion P G Koopmans; Miranda de Graaf

doi:10.1038/s41598-022-18383-6

Profiling of humoral immune responses to norovirus in children across Europe

Standard

Profiling of humoral immune responses to norovirus in children across Europe. / Villabruna, Nele; Izquierdo-Lara, Ray W; Schapendonk, Claudia M E; de Bruin, Erwin; Chandler, Felicity; Thao, Tran Thi Nhu; Westerhuis, Brenda M; van Beek, Janko; Sigfrid, Louise; Giaquinto, Carlo; Goossens, Herman; Bielicki, Julia A; Kohns Vasconcelos, Malte; Fraaij, Pieter L A; Koopmans, Marion P G; de Graaf, Miranda.

in: SCI REP-UK, Jahrgang 12, Nr. 1, 14275, 22.08.2022.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Villabruna, N, Izquierdo-Lara, RW, Schapendonk, CME, de Bruin, E, Chandler, F, Thao, TTN, Westerhuis, BM, van Beek, J, Sigfrid, L, Giaquinto, C, Goossens, H, Bielicki, JA, Kohns Vasconcelos, M, Fraaij, PLA, Koopmans, MPG & de Graaf, M 2022, 'Profiling of humoral immune responses to norovirus in children across Europe', SCI REP-UK, Jg. 12, Nr. 1, 14275. https://doi.org/10.1038/s41598-022-18383-6

APA

Villabruna, N., Izquierdo-Lara, R. W., Schapendonk, C. M. E., de Bruin, E., Chandler, F., Thao, T. T. N., Westerhuis, B. M., van Beek, J., Sigfrid, L., Giaquinto, C., Goossens, H., Bielicki, J. A., Kohns Vasconcelos, M., Fraaij, P. L. A., Koopmans, M. P. G., & de Graaf, M. (2022). Profiling of humoral immune responses to norovirus in children across Europe. SCI REP-UK, 12(1), [14275]. https://doi.org/10.1038/s41598-022-18383-6

Vancouver

Villabruna N, Izquierdo-Lara RW, Schapendonk CME, de Bruin E, Chandler F, Thao TTN et al. Profiling of humoral immune responses to norovirus in children across Europe. SCI REP-UK. 2022 Aug 22;12(1). 14275. https://doi.org/10.1038/s41598-022-18383-6

Bibtex

@article{c0cc4591e5cf4495a20b2749c2e6f636,

title = "Profiling of humoral immune responses to norovirus in children across Europe",

abstract = "Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.",

keywords = "Caliciviridae Infections/immunology, Capsid Proteins/genetics, Child, Preschool, Europe, Gastroenteritis/immunology, Genotype, Humans, Immunity, Humoral, Infant, Norovirus/genetics, Phylogeny",

author = "Nele Villabruna and Izquierdo-Lara, {Ray W} and Schapendonk, {Claudia M E} and {de Bruin}, Erwin and Felicity Chandler and Thao, {Tran Thi Nhu} and Westerhuis, {Brenda M} and {van Beek}, Janko and Louise Sigfrid and Carlo Giaquinto and Herman Goossens and Bielicki, {Julia A} and {Kohns Vasconcelos}, Malte and Fraaij, {Pieter L A} and Koopmans, {Marion P G} and {de Graaf}, Miranda",

note = "{\textcopyright} 2022. The Author(s).",

year = "2022",

month = aug,

day = "22",

doi = "10.1038/s41598-022-18383-6",

language = "English",

volume = "12",

journal = "SCI REP-UK",

issn = "2045-2322",

publisher = "NATURE PUBLISHING GROUP",

number = "1",

}

RIS

TY - JOUR

T1 - Profiling of humoral immune responses to norovirus in children across Europe

AU - Villabruna, Nele

AU - Izquierdo-Lara, Ray W

AU - Schapendonk, Claudia M E

AU - de Bruin, Erwin

AU - Chandler, Felicity

AU - Thao, Tran Thi Nhu

AU - Westerhuis, Brenda M

AU - van Beek, Janko

AU - Sigfrid, Louise

AU - Giaquinto, Carlo

AU - Goossens, Herman

AU - Bielicki, Julia A

AU - Kohns Vasconcelos, Malte

AU - Fraaij, Pieter L A

AU - Koopmans, Marion P G

AU - de Graaf, Miranda

PY - 2022/8/22

Y1 - 2022/8/22

N2 - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.

AB - Norovirus is a leading cause of epidemic acute gastroenteritis. More than 30 genotypes circulate in humans, some are common, and others are only sporadically detected. Here, we investigated whether serology can be used to determine which genotypes infect children. We established a multiplex protein microarray with structural and non-structural norovirus antigens that allowed simultaneous antibody testing against 30 human GI and GII genotypes. Antibody responses of sera obtained from 287 children aged < 1 month to 5.5 years were profiled. Most specific IgG and IgA responses were directed against the GII.2, GII.3, GII.4, and GII.6 capsid genotypes. While we detected antibody responses against rare genotypes, we found no evidence for wide circulation. We also detected genotype-specific antibodies against the non-structural proteins p48 and p22 in sera of older children. In this study, we show the age-dependent antibody responses to a broad range of norovirus capsid and polymerase genotypes, which will aid in the development of vaccines.

KW - Caliciviridae Infections/immunology

KW - Capsid Proteins/genetics

KW - Child, Preschool

KW - Europe

KW - Gastroenteritis/immunology

KW - Genotype

KW - Humans

KW - Immunity, Humoral

KW - Infant

KW - Norovirus/genetics

KW - Phylogeny

U2 - 10.1038/s41598-022-18383-6

DO - 10.1038/s41598-022-18383-6

M3 - SCORING: Journal article

C2 - 35995986

VL - 12

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

IS - 1

M1 - 14275

ER -