Research ExplorerPublicationsProbing cytoskeletal modulation of passive and active intrac...

Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots

Standard

Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots. / Katrukha, Eugene A.; Mikhaylova, Marina; van Brakel, Hugo X; van Bergen en Henegouwen, Paul M. P.; Akhmanova, Anna; Hoogenraad, Casper C.; Kapitein, Lukas C.

In: NAT COMMUN, Vol. 8, 21.03.2017, p. 14772.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Katrukha, EA, Mikhaylova, M, van Brakel, HX, van Bergen en Henegouwen, PMP, Akhmanova, A, Hoogenraad, CC & Kapitein, LC 2017, 'Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots', NAT COMMUN, vol. 8, pp. 14772. https://doi.org/10.1038/ncomms14772

APA

Katrukha, E. A., Mikhaylova, M., van Brakel, H. X., van Bergen en Henegouwen, P. M. P., Akhmanova, A., Hoogenraad, C. C., & Kapitein, L. C. (2017). Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots. NAT COMMUN, 8, 14772. https://doi.org/10.1038/ncomms14772

Vancouver

Katrukha EA, Mikhaylova M, van Brakel HX, van Bergen en Henegouwen PMP, Akhmanova A, Hoogenraad CC et al. Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots. NAT COMMUN. 2017 Mar 21;8:14772. https://doi.org/10.1038/ncomms14772

Bibtex

@article{dd031d04968d4e4eb8a7c8ea3670f473,
title = "Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots",
abstract = "The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.",
keywords = "Journal Article",
author = "Katrukha, {Eugene A.} and Marina Mikhaylova and {van Brakel}, {Hugo X} and {van Bergen en Henegouwen}, {Paul M. P.} and Anna Akhmanova and Hoogenraad, {Casper C.} and Kapitein, {Lukas C.}",
year = "2017",
month = mar,
day = "21",
doi = "10.1038/ncomms14772",
language = "English",
volume = "8",
pages = "14772",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots

AU - Katrukha, Eugene A.

AU - Mikhaylova, Marina

AU - van Brakel, Hugo X

AU - van Bergen en Henegouwen, Paul M. P.

AU - Akhmanova, Anna

AU - Hoogenraad, Casper C.

AU - Kapitein, Lukas C.

PY - 2017/3/21

Y1 - 2017/3/21

N2 - The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.

AB - The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.

KW - Journal Article

U2 - 10.1038/ncomms14772

DO - 10.1038/ncomms14772

M3 - SCORING: Journal article

C2 - 28322225

VL - 8

SP - 14772

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

ER -