Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots
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Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots. / Katrukha, Eugene A.; Mikhaylova, Marina; van Brakel, Hugo X; van Bergen en Henegouwen, Paul M. P.; Akhmanova, Anna; Hoogenraad, Casper C.; Kapitein, Lukas C.
in: NAT COMMUN, Jahrgang 8, 21.03.2017, S. 14772.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots
AU - Katrukha, Eugene A.
AU - Mikhaylova, Marina
AU - van Brakel, Hugo X
AU - van Bergen en Henegouwen, Paul M. P.
AU - Akhmanova, Anna
AU - Hoogenraad, Casper C.
AU - Kapitein, Lukas C.
PY - 2017/3/21
Y1 - 2017/3/21
N2 - The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.
AB - The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.
KW - Journal Article
U2 - 10.1038/ncomms14772
DO - 10.1038/ncomms14772
M3 - SCORING: Journal article
C2 - 28322225
VL - 8
SP - 14772
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
ER -