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Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience

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Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience. / Englhard, Anna; Girschick, Susanne; Mack, Brigitte; Volgger, Veronika; Gires, Olivier; Conderman, Christian; Stepp, Herbert; Betz, Christian Stephan.

HEAD AND NECK OPTICAL DIAGNOSTICS. ed. / C Betz; BJF Wong. Vol. 8805 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA : SPIE-INT SOC OPTICAL ENGINEERING, 2013. (Proceedings of SPIE).

Research output: SCORING: Contribution to book/anthology › SCORING: Contribution to collected editions/anthologies › Research › peer-review

Harvard

Englhard, A, Girschick, S, Mack, B, Volgger, V, Gires, O, Conderman, C, Stepp, H & Betz, CS 2013, Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience. in C Betz & BJF Wong (eds), HEAD AND NECK OPTICAL DIAGNOSTICS. vol. 8805, Proceedings of SPIE, SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA. https://doi.org/10.1117/12.2032628

APA

Englhard, A., Girschick, S., Mack, B., Volgger, V., Gires, O., Conderman, C., Stepp, H., & Betz, C. S. (2013). Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience. In C. Betz, & BJF. Wong (Eds.), HEAD AND NECK OPTICAL DIAGNOSTICS (Vol. 8805). (Proceedings of SPIE). SPIE-INT SOC OPTICAL ENGINEERING. https://doi.org/10.1117/12.2032628

Vancouver

Englhard A, Girschick S, Mack B, Volgger V, Gires O, Conderman C et al. Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience. In Betz C, Wong BJF, editors, HEAD AND NECK OPTICAL DIAGNOSTICS. Vol. 8805. 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA: SPIE-INT SOC OPTICAL ENGINEERING. 2013. (Proceedings of SPIE). https://doi.org/10.1117/12.2032628

Bibtex

@inbook{a479c7efdeb1416d8bc31579b755a939,
title = "Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience",
abstract = "Background: Malignancies of the upper aerodigestive tract (UADT) are conventionally diagnosed by white light endoscopy, biopsy and histopathology. Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel non-invasive technique which offers in vivo surface and sub-surface imaging of tissue. It produces pictures of cellular architecture comparable to histology without the need for biopsy. It has already been successfully used in different clinical subspecialties to help in the diagnosis and treatment planning of inflammatory and neoplastic diseases. PCLE needs to be used in combination with specific or non-specific contrast agents. In this study we evaluated the potential use of pCLE in combination with non-specific and specific contrast agents to distinguish between healthy mucosa and invasive carcinoma. Methods: Tissue samples from healthy mucosa and squamous cell carcinoma of the head and neck were taken during surgery. After topical application of three different contrast agents, samples were examined using different pCLE-probes and a Confocal Laser Scanning Microscope (CLSM). Images were then compared to the corresponding histological slides and cryosections. Results: Initial results show that pCLE in combination with fluorophores allows visualization of cellular and structural components. Imaging of different layers was possible using three distinct pCLE-probes. Conclusion: pCLE is a promising non-invasive technique that may be a useful adjunct in the evaluation, diagnosis and treatment planning of head and neck malignancies.",
keywords = "Head and neck squamous cell carcinoma (HNSCC), Confocal Microscopy, fluorescence, EGFR",
author = "Anna Englhard and Susanne Girschick and Brigitte Mack and Veronika Volgger and Olivier Gires and Christian Conderman and Herbert Stepp and Betz, {Christian Stephan}",
note = "5th Scientific Meeting of the Head-and-Neck-Optical-Diagnostic-Society (HNODS), Munich, GERMANY, MAY 13-14, 2013",
year = "2013",
doi = "10.1117/12.2032628",
language = "English",
isbn = "978-0-8194-9813-7",
volume = "8805",
series = "Proceedings of SPIE",
publisher = "SPIE-INT SOC OPTICAL ENGINEERING",
editor = "C Betz and BJF Wong",
booktitle = "HEAD AND NECK OPTICAL DIAGNOSTICS",

}

RIS

TY - CHAP

T1 - Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience

AU - Englhard, Anna

AU - Girschick, Susanne

AU - Mack, Brigitte

AU - Volgger, Veronika

AU - Gires, Olivier

AU - Conderman, Christian

AU - Stepp, Herbert

AU - Betz, Christian Stephan

N1 - 5th Scientific Meeting of the Head-and-Neck-Optical-Diagnostic-Society (HNODS), Munich, GERMANY, MAY 13-14, 2013

PY - 2013

Y1 - 2013

N2 - Background: Malignancies of the upper aerodigestive tract (UADT) are conventionally diagnosed by white light endoscopy, biopsy and histopathology. Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel non-invasive technique which offers in vivo surface and sub-surface imaging of tissue. It produces pictures of cellular architecture comparable to histology without the need for biopsy. It has already been successfully used in different clinical subspecialties to help in the diagnosis and treatment planning of inflammatory and neoplastic diseases. PCLE needs to be used in combination with specific or non-specific contrast agents. In this study we evaluated the potential use of pCLE in combination with non-specific and specific contrast agents to distinguish between healthy mucosa and invasive carcinoma. Methods: Tissue samples from healthy mucosa and squamous cell carcinoma of the head and neck were taken during surgery. After topical application of three different contrast agents, samples were examined using different pCLE-probes and a Confocal Laser Scanning Microscope (CLSM). Images were then compared to the corresponding histological slides and cryosections. Results: Initial results show that pCLE in combination with fluorophores allows visualization of cellular and structural components. Imaging of different layers was possible using three distinct pCLE-probes. Conclusion: pCLE is a promising non-invasive technique that may be a useful adjunct in the evaluation, diagnosis and treatment planning of head and neck malignancies.

AB - Background: Malignancies of the upper aerodigestive tract (UADT) are conventionally diagnosed by white light endoscopy, biopsy and histopathology. Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel non-invasive technique which offers in vivo surface and sub-surface imaging of tissue. It produces pictures of cellular architecture comparable to histology without the need for biopsy. It has already been successfully used in different clinical subspecialties to help in the diagnosis and treatment planning of inflammatory and neoplastic diseases. PCLE needs to be used in combination with specific or non-specific contrast agents. In this study we evaluated the potential use of pCLE in combination with non-specific and specific contrast agents to distinguish between healthy mucosa and invasive carcinoma. Methods: Tissue samples from healthy mucosa and squamous cell carcinoma of the head and neck were taken during surgery. After topical application of three different contrast agents, samples were examined using different pCLE-probes and a Confocal Laser Scanning Microscope (CLSM). Images were then compared to the corresponding histological slides and cryosections. Results: Initial results show that pCLE in combination with fluorophores allows visualization of cellular and structural components. Imaging of different layers was possible using three distinct pCLE-probes. Conclusion: pCLE is a promising non-invasive technique that may be a useful adjunct in the evaluation, diagnosis and treatment planning of head and neck malignancies.

KW - Head and neck squamous cell carcinoma (HNSCC)

KW - Confocal Microscopy

KW - fluorescence

KW - EGFR

U2 - 10.1117/12.2032628

DO - 10.1117/12.2032628

M3 - SCORING: Contribution to collected editions/anthologies

SN - 978-0-8194-9813-7

VL - 8805

T3 - Proceedings of SPIE

BT - HEAD AND NECK OPTICAL DIAGNOSTICS

A2 - Betz, C

A2 - Wong, BJF

PB - SPIE-INT SOC OPTICAL ENGINEERING

CY - 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

ER -