Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience
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Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience. / Englhard, Anna; Girschick, Susanne; Mack, Brigitte; Volgger, Veronika; Gires, Olivier; Conderman, Christian; Stepp, Herbert; Betz, Christian Stephan.
HEAD AND NECK OPTICAL DIAGNOSTICS. Hrsg. / C Betz; BJF Wong. Band 8805 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA : SPIE-INT SOC OPTICAL ENGINEERING, 2013. (Proceedings of SPIE).Publikationen: SCORING: Beitrag in Buch/Sammelwerk › SCORING: Beitrag in Sammelwerk › Forschung › Begutachtung
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T1 - Probe-based Confocal Laser Endomicroscopy in head and neck malignancies: early preclinical experience
AU - Englhard, Anna
AU - Girschick, Susanne
AU - Mack, Brigitte
AU - Volgger, Veronika
AU - Gires, Olivier
AU - Conderman, Christian
AU - Stepp, Herbert
AU - Betz, Christian Stephan
N1 - 5th Scientific Meeting of the Head-and-Neck-Optical-Diagnostic-Society (HNODS), Munich, GERMANY, MAY 13-14, 2013
PY - 2013
Y1 - 2013
N2 - Background: Malignancies of the upper aerodigestive tract (UADT) are conventionally diagnosed by white light endoscopy, biopsy and histopathology. Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel non-invasive technique which offers in vivo surface and sub-surface imaging of tissue. It produces pictures of cellular architecture comparable to histology without the need for biopsy. It has already been successfully used in different clinical subspecialties to help in the diagnosis and treatment planning of inflammatory and neoplastic diseases. PCLE needs to be used in combination with specific or non-specific contrast agents. In this study we evaluated the potential use of pCLE in combination with non-specific and specific contrast agents to distinguish between healthy mucosa and invasive carcinoma. Methods: Tissue samples from healthy mucosa and squamous cell carcinoma of the head and neck were taken during surgery. After topical application of three different contrast agents, samples were examined using different pCLE-probes and a Confocal Laser Scanning Microscope (CLSM). Images were then compared to the corresponding histological slides and cryosections. Results: Initial results show that pCLE in combination with fluorophores allows visualization of cellular and structural components. Imaging of different layers was possible using three distinct pCLE-probes. Conclusion: pCLE is a promising non-invasive technique that may be a useful adjunct in the evaluation, diagnosis and treatment planning of head and neck malignancies.
AB - Background: Malignancies of the upper aerodigestive tract (UADT) are conventionally diagnosed by white light endoscopy, biopsy and histopathology. Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel non-invasive technique which offers in vivo surface and sub-surface imaging of tissue. It produces pictures of cellular architecture comparable to histology without the need for biopsy. It has already been successfully used in different clinical subspecialties to help in the diagnosis and treatment planning of inflammatory and neoplastic diseases. PCLE needs to be used in combination with specific or non-specific contrast agents. In this study we evaluated the potential use of pCLE in combination with non-specific and specific contrast agents to distinguish between healthy mucosa and invasive carcinoma. Methods: Tissue samples from healthy mucosa and squamous cell carcinoma of the head and neck were taken during surgery. After topical application of three different contrast agents, samples were examined using different pCLE-probes and a Confocal Laser Scanning Microscope (CLSM). Images were then compared to the corresponding histological slides and cryosections. Results: Initial results show that pCLE in combination with fluorophores allows visualization of cellular and structural components. Imaging of different layers was possible using three distinct pCLE-probes. Conclusion: pCLE is a promising non-invasive technique that may be a useful adjunct in the evaluation, diagnosis and treatment planning of head and neck malignancies.
KW - Head and neck squamous cell carcinoma (HNSCC)
KW - Confocal Microscopy
KW - fluorescence
KW - EGFR
U2 - 10.1117/12.2032628
DO - 10.1117/12.2032628
M3 - SCORING: Contribution to collected editions/anthologies
SN - 978-0-8194-9813-7
VL - 8805
T3 - Proceedings of SPIE
BT - HEAD AND NECK OPTICAL DIAGNOSTICS
A2 - Betz, C
A2 - Wong, BJF
PB - SPIE-INT SOC OPTICAL ENGINEERING
CY - 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA
ER -