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Principle and applications of the protein-purification-parameter screening system

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Principle and applications of the protein-purification-parameter screening system. / Thiemann, Joachim; Jankowski, Joachim; Rykl, Jana; Kurzawski, Sandra; Pohl, Thomas; Wittmann-Liebold, Brigitte; Schlüter, Hartmut.

In: J CHROMATOGR A, Vol. 1043, No. 1, 16.07.2004, p. 73-80.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Thiemann, J, Jankowski, J, Rykl, J, Kurzawski, S, Pohl, T, Wittmann-Liebold, B & Schlüter, H 2004, 'Principle and applications of the protein-purification-parameter screening system', J CHROMATOGR A, vol. 1043, no. 1, pp. 73-80.

APA

Thiemann, J., Jankowski, J., Rykl, J., Kurzawski, S., Pohl, T., Wittmann-Liebold, B., & Schlüter, H. (2004). Principle and applications of the protein-purification-parameter screening system. J CHROMATOGR A, 1043(1), 73-80.

Vancouver

Thiemann J, Jankowski J, Rykl J, Kurzawski S, Pohl T, Wittmann-Liebold B et al. Principle and applications of the protein-purification-parameter screening system. J CHROMATOGR A. 2004 Jul 16;1043(1):73-80.

Bibtex

@article{a1c1784127724ef6b19539cf73f6bd7f,
title = "Principle and applications of the protein-purification-parameter screening system",
abstract = "For the purification of a target protein, liquid chromatography is the method of choice, if its activity has to be maintained. The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is described, which utilizes automated parallel chromatographic experiments in the batch mode in 96-well plates. The principle of this protein-purification-parameter screening (PPS) system is demonstrated with a mixture of four proteins. An application of PPS for the determination of a purification step of an angiotensin-II-generating enzyme from a crude tissue extract is shown.",
keywords = "Animals, Chromatography, Ion Exchange, Electrophoresis, Gel, Two-Dimensional, Kidney, Proteins, Swine, Journal Article, Research Support, Non-U.S. Gov't",
author = "Joachim Thiemann and Joachim Jankowski and Jana Rykl and Sandra Kurzawski and Thomas Pohl and Brigitte Wittmann-Liebold and Hartmut Schl{\"u}ter",
year = "2004",
month = jul,
day = "16",
language = "English",
volume = "1043",
pages = "73--80",
journal = "J CHROMATOGR A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Principle and applications of the protein-purification-parameter screening system

AU - Thiemann, Joachim

AU - Jankowski, Joachim

AU - Rykl, Jana

AU - Kurzawski, Sandra

AU - Pohl, Thomas

AU - Wittmann-Liebold, Brigitte

AU - Schlüter, Hartmut

PY - 2004/7/16

Y1 - 2004/7/16

N2 - For the purification of a target protein, liquid chromatography is the method of choice, if its activity has to be maintained. The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is described, which utilizes automated parallel chromatographic experiments in the batch mode in 96-well plates. The principle of this protein-purification-parameter screening (PPS) system is demonstrated with a mixture of four proteins. An application of PPS for the determination of a purification step of an angiotensin-II-generating enzyme from a crude tissue extract is shown.

AB - For the purification of a target protein, liquid chromatography is the method of choice, if its activity has to be maintained. The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is described, which utilizes automated parallel chromatographic experiments in the batch mode in 96-well plates. The principle of this protein-purification-parameter screening (PPS) system is demonstrated with a mixture of four proteins. An application of PPS for the determination of a purification step of an angiotensin-II-generating enzyme from a crude tissue extract is shown.

KW - Animals

KW - Chromatography, Ion Exchange

KW - Electrophoresis, Gel, Two-Dimensional

KW - Kidney

KW - Proteins

KW - Swine

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 15317415

VL - 1043

SP - 73

EP - 80

JO - J CHROMATOGR A

JF - J CHROMATOGR A

SN - 0021-9673

IS - 1

ER -