Principle and applications of the protein-purification-parameter screening system
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Principle and applications of the protein-purification-parameter screening system. / Thiemann, Joachim; Jankowski, Joachim; Rykl, Jana; Kurzawski, Sandra; Pohl, Thomas; Wittmann-Liebold, Brigitte; Schlüter, Hartmut.
in: J CHROMATOGR A, Jahrgang 1043, Nr. 1, 16.07.2004, S. 73-80.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Principle and applications of the protein-purification-parameter screening system
AU - Thiemann, Joachim
AU - Jankowski, Joachim
AU - Rykl, Jana
AU - Kurzawski, Sandra
AU - Pohl, Thomas
AU - Wittmann-Liebold, Brigitte
AU - Schlüter, Hartmut
PY - 2004/7/16
Y1 - 2004/7/16
N2 - For the purification of a target protein, liquid chromatography is the method of choice, if its activity has to be maintained. The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is described, which utilizes automated parallel chromatographic experiments in the batch mode in 96-well plates. The principle of this protein-purification-parameter screening (PPS) system is demonstrated with a mixture of four proteins. An application of PPS for the determination of a purification step of an angiotensin-II-generating enzyme from a crude tissue extract is shown.
AB - For the purification of a target protein, liquid chromatography is the method of choice, if its activity has to be maintained. The selection of optimum parameters will improve in proportion to the number of individual parameters varied in initial experiments. Here a fast screening method is described, which utilizes automated parallel chromatographic experiments in the batch mode in 96-well plates. The principle of this protein-purification-parameter screening (PPS) system is demonstrated with a mixture of four proteins. An application of PPS for the determination of a purification step of an angiotensin-II-generating enzyme from a crude tissue extract is shown.
KW - Animals
KW - Chromatography, Ion Exchange
KW - Electrophoresis, Gel, Two-Dimensional
KW - Kidney
KW - Proteins
KW - Swine
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 15317415
VL - 1043
SP - 73
EP - 80
JO - J CHROMATOGR A
JF - J CHROMATOGR A
SN - 0021-9673
IS - 1
ER -