Research ExplorerPublicationsPretreatment of bone with osteoclasts affects phenotypic exp...

Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells.

Standard

Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells. / Boyan, B D; Schwartz, Z; Lohmann, Christoph; Sylvia, V L; Cochran, D L; Dean, D D; Puzas, J E.

In: J ORTHOP RES, Vol. 21, No. 4, 4, 2003, p. 638-647.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Boyan, BD, Schwartz, Z, Lohmann, C, Sylvia, VL, Cochran, DL, Dean, DD & Puzas, JE 2003, 'Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells.', J ORTHOP RES, vol. 21, no. 4, 4, pp. 638-647. <http://www.ncbi.nlm.nih.gov/pubmed/12798063?dopt=Citation>

APA

Boyan, B. D., Schwartz, Z., Lohmann, C., Sylvia, V. L., Cochran, D. L., Dean, D. D., & Puzas, J. E. (2003). Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells. J ORTHOP RES, 21(4), 638-647. [4]. http://www.ncbi.nlm.nih.gov/pubmed/12798063?dopt=Citation

Vancouver

Boyan BD, Schwartz Z, Lohmann C, Sylvia VL, Cochran DL, Dean DD et al. Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells. J ORTHOP RES. 2003;21(4):638-647. 4.

Bibtex

@article{d98d508a19fa44abb64a13daec39a2f2,
title = "Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells.",
abstract = "Implant surface morphology regulates osteoblast phenotypic expression. Osteoblast sensitivity to non-biologic surfaces suggests that native bone surface features may also affect osteoblast response. To test this, MG63 osteoblast-like cells were grown for 7 days on bovine cortical bone wafers pretreated with rat bone marrow osteoclasts for 0, 10 or 20 days. Response to osteoclast-treated surfaces was compared to the response of MG63 cells to titanium surfaces with smooth and rough microtopographies. Cell number, differentiation (alkaline phosphatase activity and osteocalcin levels), and local factors (PGE(2) and TGF-beta1) were measured in confluent cultures. Compared to culture on plastic, cell number was reduced on all three types of bone wafers; this effect was dose-dependent with increasing resorption of the surface. Alkaline phosphatase specific activity was increased (P",
author = "Boyan, {B D} and Z Schwartz and Christoph Lohmann and Sylvia, {V L} and Cochran, {D L} and Dean, {D D} and Puzas, {J E}",
year = "2003",
language = "Deutsch",
volume = "21",
pages = "638--647",
journal = "J ORTHOP RES",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells.

AU - Boyan, B D

AU - Schwartz, Z

AU - Lohmann, Christoph

AU - Sylvia, V L

AU - Cochran, D L

AU - Dean, D D

AU - Puzas, J E

PY - 2003

Y1 - 2003

N2 - Implant surface morphology regulates osteoblast phenotypic expression. Osteoblast sensitivity to non-biologic surfaces suggests that native bone surface features may also affect osteoblast response. To test this, MG63 osteoblast-like cells were grown for 7 days on bovine cortical bone wafers pretreated with rat bone marrow osteoclasts for 0, 10 or 20 days. Response to osteoclast-treated surfaces was compared to the response of MG63 cells to titanium surfaces with smooth and rough microtopographies. Cell number, differentiation (alkaline phosphatase activity and osteocalcin levels), and local factors (PGE(2) and TGF-beta1) were measured in confluent cultures. Compared to culture on plastic, cell number was reduced on all three types of bone wafers; this effect was dose-dependent with increasing resorption of the surface. Alkaline phosphatase specific activity was increased (P

AB - Implant surface morphology regulates osteoblast phenotypic expression. Osteoblast sensitivity to non-biologic surfaces suggests that native bone surface features may also affect osteoblast response. To test this, MG63 osteoblast-like cells were grown for 7 days on bovine cortical bone wafers pretreated with rat bone marrow osteoclasts for 0, 10 or 20 days. Response to osteoclast-treated surfaces was compared to the response of MG63 cells to titanium surfaces with smooth and rough microtopographies. Cell number, differentiation (alkaline phosphatase activity and osteocalcin levels), and local factors (PGE(2) and TGF-beta1) were measured in confluent cultures. Compared to culture on plastic, cell number was reduced on all three types of bone wafers; this effect was dose-dependent with increasing resorption of the surface. Alkaline phosphatase specific activity was increased (P

M3 - SCORING: Zeitschriftenaufsatz

VL - 21

SP - 638

EP - 647

JO - J ORTHOP RES

JF - J ORTHOP RES

SN - 0736-0266

IS - 4

M1 - 4

ER -