Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells.
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Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells. / Boyan, B D; Schwartz, Z; Lohmann, Christoph; Sylvia, V L; Cochran, D L; Dean, D D; Puzas, J E.
in: J ORTHOP RES, Jahrgang 21, Nr. 4, 4, 2003, S. 638-647.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Pretreatment of bone with osteoclasts affects phenotypic expression of osteoblast-like cells.
AU - Boyan, B D
AU - Schwartz, Z
AU - Lohmann, Christoph
AU - Sylvia, V L
AU - Cochran, D L
AU - Dean, D D
AU - Puzas, J E
PY - 2003
Y1 - 2003
N2 - Implant surface morphology regulates osteoblast phenotypic expression. Osteoblast sensitivity to non-biologic surfaces suggests that native bone surface features may also affect osteoblast response. To test this, MG63 osteoblast-like cells were grown for 7 days on bovine cortical bone wafers pretreated with rat bone marrow osteoclasts for 0, 10 or 20 days. Response to osteoclast-treated surfaces was compared to the response of MG63 cells to titanium surfaces with smooth and rough microtopographies. Cell number, differentiation (alkaline phosphatase activity and osteocalcin levels), and local factors (PGE(2) and TGF-beta1) were measured in confluent cultures. Compared to culture on plastic, cell number was reduced on all three types of bone wafers; this effect was dose-dependent with increasing resorption of the surface. Alkaline phosphatase specific activity was increased (P
AB - Implant surface morphology regulates osteoblast phenotypic expression. Osteoblast sensitivity to non-biologic surfaces suggests that native bone surface features may also affect osteoblast response. To test this, MG63 osteoblast-like cells were grown for 7 days on bovine cortical bone wafers pretreated with rat bone marrow osteoclasts for 0, 10 or 20 days. Response to osteoclast-treated surfaces was compared to the response of MG63 cells to titanium surfaces with smooth and rough microtopographies. Cell number, differentiation (alkaline phosphatase activity and osteocalcin levels), and local factors (PGE(2) and TGF-beta1) were measured in confluent cultures. Compared to culture on plastic, cell number was reduced on all three types of bone wafers; this effect was dose-dependent with increasing resorption of the surface. Alkaline phosphatase specific activity was increased (P
M3 - SCORING: Zeitschriftenaufsatz
VL - 21
SP - 638
EP - 647
JO - J ORTHOP RES
JF - J ORTHOP RES
SN - 0736-0266
IS - 4
M1 - 4
ER -