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Podosome reformation in macrophages: assays and analysis

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Podosome reformation in macrophages: assays and analysis. / Cervero, Pasquale; Panzer, Linda; Linder, Stefan.

In: Methods Mol Biol, Vol. 1046, 01.01.2013, p. 97-121.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Cervero, P, Panzer, L & Linder, S 2013, 'Podosome reformation in macrophages: assays and analysis', Methods Mol Biol, vol. 1046, pp. 97-121. https://doi.org/10.1007/978-1-62703-538-5_6

APA

Cervero, P., Panzer, L., & Linder, S. (2013). Podosome reformation in macrophages: assays and analysis. Methods Mol Biol, 1046, 97-121. https://doi.org/10.1007/978-1-62703-538-5_6

Vancouver

Cervero P, Panzer L, Linder S. Podosome reformation in macrophages: assays and analysis. Methods Mol Biol. 2013 Jan 1;1046:97-121. https://doi.org/10.1007/978-1-62703-538-5_6

Bibtex

@article{bbc5a6d361a84b53bed78193405b2a70,
title = "Podosome reformation in macrophages: assays and analysis",
abstract = "Podosomes are multifunctional organelles of invasive cells that combine several key abilities including cell-matrix adhesion, extracellular matrix degradation, and mechanosensing. In combination with their high turnover rates that allow quick adaptation to the pericellular environment, podosomes are likely to play important roles during invasive migration of cells. Primary human macrophages constitutively form numerous podosomes and are thus an ideal system for the quantitative study of podosome dynamics. This protocol describes assays for the study of podosome dynamics, namely, reformation of podosomes, in fixed and living cells, with subsequent software-based analyses allowing the extraction of quantitative parameters such as the number of podosomes per cell, podosome density, and half times for podosome disruption and reformation. Moreover, we describe the preparation of podosome-enriched cell fractions and their analysis by immunoblotting.",
keywords = "Biological Assay, Cell Fractionation, Cell Movement, Cell-Matrix Junctions, Extracellular Matrix, Humans, Macrophages, Molecular Biology, Neoplasm Invasiveness",
author = "Pasquale Cervero and Linda Panzer and Stefan Linder",
year = "2013",
month = jan,
day = "1",
doi = "10.1007/978-1-62703-538-5_6",
language = "English",
volume = "1046",
pages = "97--121",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Podosome reformation in macrophages: assays and analysis

AU - Cervero, Pasquale

AU - Panzer, Linda

AU - Linder, Stefan

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Podosomes are multifunctional organelles of invasive cells that combine several key abilities including cell-matrix adhesion, extracellular matrix degradation, and mechanosensing. In combination with their high turnover rates that allow quick adaptation to the pericellular environment, podosomes are likely to play important roles during invasive migration of cells. Primary human macrophages constitutively form numerous podosomes and are thus an ideal system for the quantitative study of podosome dynamics. This protocol describes assays for the study of podosome dynamics, namely, reformation of podosomes, in fixed and living cells, with subsequent software-based analyses allowing the extraction of quantitative parameters such as the number of podosomes per cell, podosome density, and half times for podosome disruption and reformation. Moreover, we describe the preparation of podosome-enriched cell fractions and their analysis by immunoblotting.

AB - Podosomes are multifunctional organelles of invasive cells that combine several key abilities including cell-matrix adhesion, extracellular matrix degradation, and mechanosensing. In combination with their high turnover rates that allow quick adaptation to the pericellular environment, podosomes are likely to play important roles during invasive migration of cells. Primary human macrophages constitutively form numerous podosomes and are thus an ideal system for the quantitative study of podosome dynamics. This protocol describes assays for the study of podosome dynamics, namely, reformation of podosomes, in fixed and living cells, with subsequent software-based analyses allowing the extraction of quantitative parameters such as the number of podosomes per cell, podosome density, and half times for podosome disruption and reformation. Moreover, we describe the preparation of podosome-enriched cell fractions and their analysis by immunoblotting.

KW - Biological Assay

KW - Cell Fractionation

KW - Cell Movement

KW - Cell-Matrix Junctions

KW - Extracellular Matrix

KW - Humans

KW - Macrophages

KW - Molecular Biology

KW - Neoplasm Invasiveness

U2 - 10.1007/978-1-62703-538-5_6

DO - 10.1007/978-1-62703-538-5_6

M3 - SCORING: Journal article

C2 - 23868584

VL - 1046

SP - 97

EP - 121

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -