Plexin-B2 controls the development of cerebellar granule cells
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Plexin-B2 controls the development of cerebellar granule cells. / Friedel, Roland H; Kerjan, Géraldine; Rayburn, Helen; Schüller, Ulrich; Sotelo, Constantino; Tessier-Lavigne, Marc; Chédotal, Alain.
In: J NEUROSCI, Vol. 27, No. 14, 04.04.2007, p. 3921-32.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Plexin-B2 controls the development of cerebellar granule cells
AU - Friedel, Roland H
AU - Kerjan, Géraldine
AU - Rayburn, Helen
AU - Schüller, Ulrich
AU - Sotelo, Constantino
AU - Tessier-Lavigne, Marc
AU - Chédotal, Alain
PY - 2007/4/4
Y1 - 2007/4/4
N2 - Cerebellar granule cell progenitors proliferate postnatally in the upper part of the external granule cell layer (EGL) of the cerebellum. Postmitotic granule cells differentiate and migrate, tangentially in the EGL and then radially through the molecular and Purkinje cell layers. The molecular control of the transition between proliferation and differentiation in cerebellar granule cells is poorly understood. We show here that the transmembrane receptor Plexin-B2 is expressed by proliferating granule cell progenitors. To study Plexin-B2 function, we generated a targeted mutation of mouse Plexin-B2. Most Plexin-B2(-/-) mutants die at birth as a result of neural tube closure defects. Some mutants survive but their cerebellum cytoarchitecture is profoundly altered. This is correlated with a disorganization of the timing of granule cell proliferation and differentiation in the EGL. Many differentiated granule cells migrate inside the cerebellum and keep proliferating. These results reveal that Plexin-B2 controls the balance between proliferation and differentiation in granule cells.
AB - Cerebellar granule cell progenitors proliferate postnatally in the upper part of the external granule cell layer (EGL) of the cerebellum. Postmitotic granule cells differentiate and migrate, tangentially in the EGL and then radially through the molecular and Purkinje cell layers. The molecular control of the transition between proliferation and differentiation in cerebellar granule cells is poorly understood. We show here that the transmembrane receptor Plexin-B2 is expressed by proliferating granule cell progenitors. To study Plexin-B2 function, we generated a targeted mutation of mouse Plexin-B2. Most Plexin-B2(-/-) mutants die at birth as a result of neural tube closure defects. Some mutants survive but their cerebellum cytoarchitecture is profoundly altered. This is correlated with a disorganization of the timing of granule cell proliferation and differentiation in the EGL. Many differentiated granule cells migrate inside the cerebellum and keep proliferating. These results reveal that Plexin-B2 controls the balance between proliferation and differentiation in granule cells.
KW - Animals
KW - Cell Differentiation
KW - Cell Proliferation
KW - Cerebellum
KW - Female
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Knockout
KW - Mutation
KW - Nerve Tissue Proteins
KW - Receptors, Cell Surface
KW - Comparative Study
KW - Journal Article
KW - Research Support, N.I.H., Extramural
KW - Research Support, Non-U.S. Gov't
U2 - 10.1523/JNEUROSCI.4710-06.2007
DO - 10.1523/JNEUROSCI.4710-06.2007
M3 - SCORING: Journal article
C2 - 17409257
VL - 27
SP - 3921
EP - 3932
JO - J NEUROSCI
JF - J NEUROSCI
SN - 0270-6474
IS - 14
ER -