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Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection

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Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection. / Hong, Henoch S; Eberhard, Johanna M; Keudel, Phillip; Bollmann, Benjamin A; Ahmad, Fareed; Ballmaier, Matthias; Bhatnagar, Nupur; Zielinska-Skowronek, Margot; Schmidt, Reinhold E; Meyer-Olson, Dirk.

In: AIDS, Vol. 24, No. 12, 31.07.2010, p. 1823-34.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hong, HS, Eberhard, JM, Keudel, P, Bollmann, BA, Ahmad, F, Ballmaier, M, Bhatnagar, N, Zielinska-Skowronek, M, Schmidt, RE & Meyer-Olson, D 2010, 'Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection', AIDS, vol. 24, no. 12, pp. 1823-34. https://doi.org/10.1097/QAD.0b013e32833b556f

APA

Hong, H. S., Eberhard, J. M., Keudel, P., Bollmann, B. A., Ahmad, F., Ballmaier, M., Bhatnagar, N., Zielinska-Skowronek, M., Schmidt, R. E., & Meyer-Olson, D. (2010). Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection. AIDS, 24(12), 1823-34. https://doi.org/10.1097/QAD.0b013e32833b556f

Vancouver

Hong HS, Eberhard JM, Keudel P, Bollmann BA, Ahmad F, Ballmaier M et al. Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection. AIDS. 2010 Jul 31;24(12):1823-34. https://doi.org/10.1097/QAD.0b013e32833b556f

Bibtex

@article{1168fe5864c54819a368fc842473079f,
title = "Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection",
abstract = "OBJECTIVE: Chronic HIV infection has been associated with activation and increased turnover of natural killer (NK) cells as well as with disturbed homeostasis of the NK cell compartment, including loss of CD56(+) NK cells and accumulation of dysfunctional CD56(-)/CD16(+) NK cells. We performed a comprehensive phenotypical and functional characterization of this population.DESIGN: A cross-sectional study was performed to analyze CD56(-)/CD16(+) NK cells from 34 untreated HIV-infected and 15 seronegative individuals.METHODS: NK cells were analyzed by flow cytometry. Degranulation was assessed by measuring their expression of CD107a after stimulation with K562 cells, interleukin-12 and interleukin-15.RESULTS: CD56(-)/CD16(+) NK cells are heterogeneous and composed of two populations, namely CD122(-)/CCR7(+) cells and CD122(-)/CCR7(+) cells. We show that expanded CD122(+) but not CCR7(+) cells in HIV-seropositive individuals are characterized by expression of senescence marker CD57 similarly to CD56(dim)/CD16(+) NK cells along with expression of KIRs, CD8, perforin and granzyme B. Despite expression of perforin and granzyme B, CD57 expressing cells exhibited less numbers of degranulating cells as measured by CD107a, indicating their functional impairment. However, there was no correlation between expansion of total CD56(-)/CD16(+) NK cells or the distinct subpopulations and viral load or CD4 cell count.CONCLUSION: These data indicate that expansion of CD56(-)/CD16(+) cells in HIV infection is driven by a distinct subset within this population with high expression of terminal differentiation marker with a phenotype resembling CD56(-)/CD16(+) NK cells.",
keywords = "Adult, Aged, Biomarkers, CD4 Lymphocyte Count, CD56 Antigen/genetics, Cell Proliferation, Cross-Sectional Studies, Female, Flow Cytometry, HIV Infections/genetics, Humans, Killer Cells, Natural/immunology, Lymphocyte Activation/immunology, Lymphocyte Subsets/immunology, Male, Middle Aged, Phenotype, Receptors, IgG/genetics, Viral Load, Young Adult",
author = "Hong, {Henoch S} and Eberhard, {Johanna M} and Phillip Keudel and Bollmann, {Benjamin A} and Fareed Ahmad and Matthias Ballmaier and Nupur Bhatnagar and Margot Zielinska-Skowronek and Schmidt, {Reinhold E} and Dirk Meyer-Olson",
year = "2010",
month = jul,
day = "31",
doi = "10.1097/QAD.0b013e32833b556f",
language = "English",
volume = "24",
pages = "1823--34",
journal = "AIDS",
issn = "0269-9370",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

RIS

TY - JOUR

T1 - Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection

AU - Hong, Henoch S

AU - Eberhard, Johanna M

AU - Keudel, Phillip

AU - Bollmann, Benjamin A

AU - Ahmad, Fareed

AU - Ballmaier, Matthias

AU - Bhatnagar, Nupur

AU - Zielinska-Skowronek, Margot

AU - Schmidt, Reinhold E

AU - Meyer-Olson, Dirk

PY - 2010/7/31

Y1 - 2010/7/31

N2 - OBJECTIVE: Chronic HIV infection has been associated with activation and increased turnover of natural killer (NK) cells as well as with disturbed homeostasis of the NK cell compartment, including loss of CD56(+) NK cells and accumulation of dysfunctional CD56(-)/CD16(+) NK cells. We performed a comprehensive phenotypical and functional characterization of this population.DESIGN: A cross-sectional study was performed to analyze CD56(-)/CD16(+) NK cells from 34 untreated HIV-infected and 15 seronegative individuals.METHODS: NK cells were analyzed by flow cytometry. Degranulation was assessed by measuring their expression of CD107a after stimulation with K562 cells, interleukin-12 and interleukin-15.RESULTS: CD56(-)/CD16(+) NK cells are heterogeneous and composed of two populations, namely CD122(-)/CCR7(+) cells and CD122(-)/CCR7(+) cells. We show that expanded CD122(+) but not CCR7(+) cells in HIV-seropositive individuals are characterized by expression of senescence marker CD57 similarly to CD56(dim)/CD16(+) NK cells along with expression of KIRs, CD8, perforin and granzyme B. Despite expression of perforin and granzyme B, CD57 expressing cells exhibited less numbers of degranulating cells as measured by CD107a, indicating their functional impairment. However, there was no correlation between expansion of total CD56(-)/CD16(+) NK cells or the distinct subpopulations and viral load or CD4 cell count.CONCLUSION: These data indicate that expansion of CD56(-)/CD16(+) cells in HIV infection is driven by a distinct subset within this population with high expression of terminal differentiation marker with a phenotype resembling CD56(-)/CD16(+) NK cells.

AB - OBJECTIVE: Chronic HIV infection has been associated with activation and increased turnover of natural killer (NK) cells as well as with disturbed homeostasis of the NK cell compartment, including loss of CD56(+) NK cells and accumulation of dysfunctional CD56(-)/CD16(+) NK cells. We performed a comprehensive phenotypical and functional characterization of this population.DESIGN: A cross-sectional study was performed to analyze CD56(-)/CD16(+) NK cells from 34 untreated HIV-infected and 15 seronegative individuals.METHODS: NK cells were analyzed by flow cytometry. Degranulation was assessed by measuring their expression of CD107a after stimulation with K562 cells, interleukin-12 and interleukin-15.RESULTS: CD56(-)/CD16(+) NK cells are heterogeneous and composed of two populations, namely CD122(-)/CCR7(+) cells and CD122(-)/CCR7(+) cells. We show that expanded CD122(+) but not CCR7(+) cells in HIV-seropositive individuals are characterized by expression of senescence marker CD57 similarly to CD56(dim)/CD16(+) NK cells along with expression of KIRs, CD8, perforin and granzyme B. Despite expression of perforin and granzyme B, CD57 expressing cells exhibited less numbers of degranulating cells as measured by CD107a, indicating their functional impairment. However, there was no correlation between expansion of total CD56(-)/CD16(+) NK cells or the distinct subpopulations and viral load or CD4 cell count.CONCLUSION: These data indicate that expansion of CD56(-)/CD16(+) cells in HIV infection is driven by a distinct subset within this population with high expression of terminal differentiation marker with a phenotype resembling CD56(-)/CD16(+) NK cells.

KW - Adult

KW - Aged

KW - Biomarkers

KW - CD4 Lymphocyte Count

KW - CD56 Antigen/genetics

KW - Cell Proliferation

KW - Cross-Sectional Studies

KW - Female

KW - Flow Cytometry

KW - HIV Infections/genetics

KW - Humans

KW - Killer Cells, Natural/immunology

KW - Lymphocyte Activation/immunology

KW - Lymphocyte Subsets/immunology

KW - Male

KW - Middle Aged

KW - Phenotype

KW - Receptors, IgG/genetics

KW - Viral Load

KW - Young Adult

U2 - 10.1097/QAD.0b013e32833b556f

DO - 10.1097/QAD.0b013e32833b556f

M3 - SCORING: Journal article

C2 - 20543659

VL - 24

SP - 1823

EP - 1834

JO - AIDS

JF - AIDS

SN - 0269-9370

IS - 12

ER -