Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection
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Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection. / Hong, Henoch S; Eberhard, Johanna M; Keudel, Phillip; Bollmann, Benjamin A; Ahmad, Fareed; Ballmaier, Matthias; Bhatnagar, Nupur; Zielinska-Skowronek, Margot; Schmidt, Reinhold E; Meyer-Olson, Dirk.
in: AIDS, Jahrgang 24, Nr. 12, 31.07.2010, S. 1823-34.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection
AU - Hong, Henoch S
AU - Eberhard, Johanna M
AU - Keudel, Phillip
AU - Bollmann, Benjamin A
AU - Ahmad, Fareed
AU - Ballmaier, Matthias
AU - Bhatnagar, Nupur
AU - Zielinska-Skowronek, Margot
AU - Schmidt, Reinhold E
AU - Meyer-Olson, Dirk
PY - 2010/7/31
Y1 - 2010/7/31
N2 - OBJECTIVE: Chronic HIV infection has been associated with activation and increased turnover of natural killer (NK) cells as well as with disturbed homeostasis of the NK cell compartment, including loss of CD56(+) NK cells and accumulation of dysfunctional CD56(-)/CD16(+) NK cells. We performed a comprehensive phenotypical and functional characterization of this population.DESIGN: A cross-sectional study was performed to analyze CD56(-)/CD16(+) NK cells from 34 untreated HIV-infected and 15 seronegative individuals.METHODS: NK cells were analyzed by flow cytometry. Degranulation was assessed by measuring their expression of CD107a after stimulation with K562 cells, interleukin-12 and interleukin-15.RESULTS: CD56(-)/CD16(+) NK cells are heterogeneous and composed of two populations, namely CD122(-)/CCR7(+) cells and CD122(-)/CCR7(+) cells. We show that expanded CD122(+) but not CCR7(+) cells in HIV-seropositive individuals are characterized by expression of senescence marker CD57 similarly to CD56(dim)/CD16(+) NK cells along with expression of KIRs, CD8, perforin and granzyme B. Despite expression of perforin and granzyme B, CD57 expressing cells exhibited less numbers of degranulating cells as measured by CD107a, indicating their functional impairment. However, there was no correlation between expansion of total CD56(-)/CD16(+) NK cells or the distinct subpopulations and viral load or CD4 cell count.CONCLUSION: These data indicate that expansion of CD56(-)/CD16(+) cells in HIV infection is driven by a distinct subset within this population with high expression of terminal differentiation marker with a phenotype resembling CD56(-)/CD16(+) NK cells.
AB - OBJECTIVE: Chronic HIV infection has been associated with activation and increased turnover of natural killer (NK) cells as well as with disturbed homeostasis of the NK cell compartment, including loss of CD56(+) NK cells and accumulation of dysfunctional CD56(-)/CD16(+) NK cells. We performed a comprehensive phenotypical and functional characterization of this population.DESIGN: A cross-sectional study was performed to analyze CD56(-)/CD16(+) NK cells from 34 untreated HIV-infected and 15 seronegative individuals.METHODS: NK cells were analyzed by flow cytometry. Degranulation was assessed by measuring their expression of CD107a after stimulation with K562 cells, interleukin-12 and interleukin-15.RESULTS: CD56(-)/CD16(+) NK cells are heterogeneous and composed of two populations, namely CD122(-)/CCR7(+) cells and CD122(-)/CCR7(+) cells. We show that expanded CD122(+) but not CCR7(+) cells in HIV-seropositive individuals are characterized by expression of senescence marker CD57 similarly to CD56(dim)/CD16(+) NK cells along with expression of KIRs, CD8, perforin and granzyme B. Despite expression of perforin and granzyme B, CD57 expressing cells exhibited less numbers of degranulating cells as measured by CD107a, indicating their functional impairment. However, there was no correlation between expansion of total CD56(-)/CD16(+) NK cells or the distinct subpopulations and viral load or CD4 cell count.CONCLUSION: These data indicate that expansion of CD56(-)/CD16(+) cells in HIV infection is driven by a distinct subset within this population with high expression of terminal differentiation marker with a phenotype resembling CD56(-)/CD16(+) NK cells.
KW - Adult
KW - Aged
KW - Biomarkers
KW - CD4 Lymphocyte Count
KW - CD56 Antigen/genetics
KW - Cell Proliferation
KW - Cross-Sectional Studies
KW - Female
KW - Flow Cytometry
KW - HIV Infections/genetics
KW - Humans
KW - Killer Cells, Natural/immunology
KW - Lymphocyte Activation/immunology
KW - Lymphocyte Subsets/immunology
KW - Male
KW - Middle Aged
KW - Phenotype
KW - Receptors, IgG/genetics
KW - Viral Load
KW - Young Adult
U2 - 10.1097/QAD.0b013e32833b556f
DO - 10.1097/QAD.0b013e32833b556f
M3 - SCORING: Journal article
C2 - 20543659
VL - 24
SP - 1823
EP - 1834
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 12
ER -