Oxalate-induced chronic kidney disease with its uremic and cardiovascular complications in C57BL/6 mice
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Oxalate-induced chronic kidney disease with its uremic and cardiovascular complications in C57BL/6 mice. / Mulay, Shrikant R; Eberhard, Jonathan N; Pfann, Victoria; Marschner, Julian A; Darisipudi, Murthy N; Daniel, Christoph; Romoli, Simone; Desai, Jyaysi; Grigorescu, Melissa; Kumar, Santhosh V; Rathkolb, Birgit; Wolf, Eckhard; Hrabě de Angelis, Martin; Bäuerle, Tobias; Dietel, Barbara; Wagner, Carsten A; Amann, Kerstin; Eckardt, Kai-Uwe; Aronson, Peter S; Anders, Hans Joachim; Knauf, Felix.
In: AM J PHYSIOL-RENAL, Vol. 310, No. 8, 15.04.2016, p. F785-F795.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Oxalate-induced chronic kidney disease with its uremic and cardiovascular complications in C57BL/6 mice
AU - Mulay, Shrikant R
AU - Eberhard, Jonathan N
AU - Pfann, Victoria
AU - Marschner, Julian A
AU - Darisipudi, Murthy N
AU - Daniel, Christoph
AU - Romoli, Simone
AU - Desai, Jyaysi
AU - Grigorescu, Melissa
AU - Kumar, Santhosh V
AU - Rathkolb, Birgit
AU - Wolf, Eckhard
AU - Hrabě de Angelis, Martin
AU - Bäuerle, Tobias
AU - Dietel, Barbara
AU - Wagner, Carsten A
AU - Amann, Kerstin
AU - Eckardt, Kai-Uwe
AU - Aronson, Peter S
AU - Anders, Hans Joachim
AU - Knauf, Felix
N1 - Copyright © 2016 the American Physiological Society.
PY - 2016/4/15
Y1 - 2016/4/15
N2 - Chronic kidney disease (CKD) research is limited by the lack of convenient inducible models mimicking human CKD and its complications in experimental animals. We demonstrate that a soluble oxalate-rich diet induces stable stages of CKD in male and female C57BL/6 mice. Renal histology is characterized by tubular damage, remnant atubular glomeruli, interstitial inflammation, and fibrosis, with the extent of tissue involvement depending on the duration of oxalate feeding. Expression profiling of markers and magnetic resonance imaging findings established to reflect inflammation and fibrosis parallel the histological changes. Within 3 wk, the mice reproducibly develop normochromic anemia, metabolic acidosis, hyperkalemia, FGF23 activation, hyperphosphatemia, and hyperparathyroidism. In addition, the model is characterized by profound arterial hypertension as well as cardiac fibrosis that persist following the switch to a control diet. Together, this new model of inducible CKD overcomes a number of previous experimental limitations and should serve useful in research related to CKD and its complications.
AB - Chronic kidney disease (CKD) research is limited by the lack of convenient inducible models mimicking human CKD and its complications in experimental animals. We demonstrate that a soluble oxalate-rich diet induces stable stages of CKD in male and female C57BL/6 mice. Renal histology is characterized by tubular damage, remnant atubular glomeruli, interstitial inflammation, and fibrosis, with the extent of tissue involvement depending on the duration of oxalate feeding. Expression profiling of markers and magnetic resonance imaging findings established to reflect inflammation and fibrosis parallel the histological changes. Within 3 wk, the mice reproducibly develop normochromic anemia, metabolic acidosis, hyperkalemia, FGF23 activation, hyperphosphatemia, and hyperparathyroidism. In addition, the model is characterized by profound arterial hypertension as well as cardiac fibrosis that persist following the switch to a control diet. Together, this new model of inducible CKD overcomes a number of previous experimental limitations and should serve useful in research related to CKD and its complications.
KW - Animals
KW - Disease Models, Animal
KW - Fibrosis
KW - Hypertension
KW - Mice
KW - Mice, Inbred C57BL
KW - Myocardium
KW - Oxalic Acid
KW - Renal Insufficiency, Chronic
KW - Uremia
KW - Journal Article
U2 - 10.1152/ajprenal.00488.2015
DO - 10.1152/ajprenal.00488.2015
M3 - SCORING: Journal article
C2 - 26764204
VL - 310
SP - F785-F795
JO - AM J PHYSIOL-RENAL
JF - AM J PHYSIOL-RENAL
SN - 1931-857X
IS - 8
ER -