Osteopontin-mediated myocardial fibrosis in heart failure: a role for lysyl oxidase?
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Osteopontin-mediated myocardial fibrosis in heart failure: a role for lysyl oxidase? / López, Begoña; González, Arantxa; Lindner, Diana; Westermann, Dirk; Ravassa, Susana; Beaumont, Javier; Gallego, Idoia; Zudaire, Amaia; Brugnolaro, Cristina; Querejeta, Ramón; Larman, Mariano; Tschöpe, Carsten; Díez, Javier.
In: CARDIOVASC RES, Vol. 99, No. 1, 01.07.2013, p. 111-120.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Osteopontin-mediated myocardial fibrosis in heart failure: a role for lysyl oxidase?
AU - López, Begoña
AU - González, Arantxa
AU - Lindner, Diana
AU - Westermann, Dirk
AU - Ravassa, Susana
AU - Beaumont, Javier
AU - Gallego, Idoia
AU - Zudaire, Amaia
AU - Brugnolaro, Cristina
AU - Querejeta, Ramón
AU - Larman, Mariano
AU - Tschöpe, Carsten
AU - Díez, Javier
PY - 2013/7/1
Y1 - 2013/7/1
N2 - AIMS: We investigated whether the pro-fibrotic matricellular protein osteopontin (OPN) is associated with the enzymes involved in the extracellular synthesis of fibril-forming collagen type I (i.e. procollagen C-proteinase, PCP) and its cross-linking to form insoluble fibrils (i.e. lysyl oxidase, LOX) in heart failure (HF) of hypertensive origin.METHODS AND RESULTS: OPN, PCP, and LOX were assessed by histochemical and molecular methods in the myocardium of 21 patients with hypertensive heart disease (HHD) and HF. Whereas the myocardial expression of OPN was very scarce in control hearts (n = 10), it was highly expressed in HF patients (P < 0.0001). OPN was directly correlated with LOX (r = 0.460, P = 0.041), insoluble collagen (r = 0.534, P = 0.015), pulmonary capillary wedge pressure (r = 0.558; P = 0.009), and left-ventricular (LV) chamber stiffness (r = 0.458, P = 0.037), and inversely correlated with LV ejection fraction (r = -0.513, P = 0.017) in all patients. OPN did not correlate with PCP and other parameters assessing collagen synthesis by fibroblasts or degradation by matrix metalloproteinases. In vitro studies showed that OPN significantly (P < 0.05) increases the expression and activity of LOX in human cardiac and dermal fibroblasts.CONCLUSION: An excess of OPN is associated with increased LOX and insoluble collagen, as well as with LV stiffness and systolic dysfunction in patients with HHD and HF. In addition, OPN up-regulates LOX in human fibroblasts. It is suggested that the OPN-LOX axis might facilitate the formation of insoluble collagen (i.e. stiff and resistant to degradation) and the subsequent alteration in LV mechanical properties and function in patients with HHD and HF.
AB - AIMS: We investigated whether the pro-fibrotic matricellular protein osteopontin (OPN) is associated with the enzymes involved in the extracellular synthesis of fibril-forming collagen type I (i.e. procollagen C-proteinase, PCP) and its cross-linking to form insoluble fibrils (i.e. lysyl oxidase, LOX) in heart failure (HF) of hypertensive origin.METHODS AND RESULTS: OPN, PCP, and LOX were assessed by histochemical and molecular methods in the myocardium of 21 patients with hypertensive heart disease (HHD) and HF. Whereas the myocardial expression of OPN was very scarce in control hearts (n = 10), it was highly expressed in HF patients (P < 0.0001). OPN was directly correlated with LOX (r = 0.460, P = 0.041), insoluble collagen (r = 0.534, P = 0.015), pulmonary capillary wedge pressure (r = 0.558; P = 0.009), and left-ventricular (LV) chamber stiffness (r = 0.458, P = 0.037), and inversely correlated with LV ejection fraction (r = -0.513, P = 0.017) in all patients. OPN did not correlate with PCP and other parameters assessing collagen synthesis by fibroblasts or degradation by matrix metalloproteinases. In vitro studies showed that OPN significantly (P < 0.05) increases the expression and activity of LOX in human cardiac and dermal fibroblasts.CONCLUSION: An excess of OPN is associated with increased LOX and insoluble collagen, as well as with LV stiffness and systolic dysfunction in patients with HHD and HF. In addition, OPN up-regulates LOX in human fibroblasts. It is suggested that the OPN-LOX axis might facilitate the formation of insoluble collagen (i.e. stiff and resistant to degradation) and the subsequent alteration in LV mechanical properties and function in patients with HHD and HF.
KW - Aged
KW - Bone Morphogenetic Protein 1/metabolism
KW - Case-Control Studies
KW - Cells, Cultured
KW - Collagen/metabolism
KW - Elasticity
KW - Female
KW - Fibroblasts/enzymology
KW - Fibrosis
KW - Heart Failure/diagnosis
KW - Humans
KW - Hypertension/complications
KW - Male
KW - Middle Aged
KW - Myocardium/enzymology
KW - Osteopontin/metabolism
KW - Protein-Lysine 6-Oxidase/metabolism
KW - Pulmonary Wedge Pressure
KW - Stroke Volume
KW - Ventricular Function, Left
U2 - 10.1093/cvr/cvt100
DO - 10.1093/cvr/cvt100
M3 - SCORING: Journal article
C2 - 23619422
VL - 99
SP - 111
EP - 120
JO - CARDIOVASC RES
JF - CARDIOVASC RES
SN - 0008-6363
IS - 1
ER -