Orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy

TY - JOUR

T1 - Orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy

AU - Stubbendorff, Mandy

AU - Deuse, Tobias

AU - Hammel, Anna

AU - Robbins, Robert C

AU - Reichenspurner, Hermann

AU - Schrepfer, Sonja

PY - 2010/12/4

Y1 - 2010/12/4

N2 - Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant vasculopathy (TVP). The commonly used animal model for cardiovascular chronic rejection studies is the heterotopic heart transplant model performed in laboratory rodents. This model is used widely in experiments since Ono and Lindsey published their technique. To analyze the findings in the blood vessels, the heart has to be sectioned and all vessels have to be measured. Another method to investigate chronic rejection in cardiovascular questionings is the aortic transplant model. In the orthotopic aortic transplant model, the aorta can easily be histologically evaluated. The PVG-to-ACI model is especially useful for CAV studies, since acute vascular rejection is not a major confounding factor and Cyclosporin A (CsA) treatment does not prevent the development of CAV, similar to what we find in the clinical setting. A7-day period of CsA is required in this model to prevent acute rejection and to achieve long-term survival with the development of TVP. This model can also be used to investigate acute cellular rejection and media necrosis in xenogeneic models.

AB - Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant vasculopathy (TVP). The commonly used animal model for cardiovascular chronic rejection studies is the heterotopic heart transplant model performed in laboratory rodents. This model is used widely in experiments since Ono and Lindsey published their technique. To analyze the findings in the blood vessels, the heart has to be sectioned and all vessels have to be measured. Another method to investigate chronic rejection in cardiovascular questionings is the aortic transplant model. In the orthotopic aortic transplant model, the aorta can easily be histologically evaluated. The PVG-to-ACI model is especially useful for CAV studies, since acute vascular rejection is not a major confounding factor and Cyclosporin A (CsA) treatment does not prevent the development of CAV, similar to what we find in the clinical setting. A7-day period of CsA is required in this model to prevent acute rejection and to achieve long-term survival with the development of TVP. This model can also be used to investigate acute cellular rejection and media necrosis in xenogeneic models.

KW - Animals

KW - Aorta/transplantation

KW - Chronic Disease

KW - Disease Models, Animal

KW - Graft Rejection/pathology

KW - Rats

KW - Rats, Inbred ACI

KW - Transplantation, Homologous/adverse effects

KW - Vascular Diseases/etiology

U2 - 10.3791/1989

DO - 10.3791/1989

M3 - SCORING: Journal article

C2 - 21178963

JO - JOVE-J VIS EXP

JF - JOVE-J VIS EXP

SN - 1940-087X

IS - 46

ER -