Orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy
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Orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy. / Stubbendorff, Mandy; Deuse, Tobias; Hammel, Anna; Robbins, Robert C; Reichenspurner, Hermann; Schrepfer, Sonja.
in: JOVE-J VIS EXP, Nr. 46, 04.12.2010.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Orthotopic aortic transplantation: a rat model to study the development of chronic vasculopathy
AU - Stubbendorff, Mandy
AU - Deuse, Tobias
AU - Hammel, Anna
AU - Robbins, Robert C
AU - Reichenspurner, Hermann
AU - Schrepfer, Sonja
PY - 2010/12/4
Y1 - 2010/12/4
N2 - Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant vasculopathy (TVP). The commonly used animal model for cardiovascular chronic rejection studies is the heterotopic heart transplant model performed in laboratory rodents. This model is used widely in experiments since Ono and Lindsey published their technique. To analyze the findings in the blood vessels, the heart has to be sectioned and all vessels have to be measured. Another method to investigate chronic rejection in cardiovascular questionings is the aortic transplant model. In the orthotopic aortic transplant model, the aorta can easily be histologically evaluated. The PVG-to-ACI model is especially useful for CAV studies, since acute vascular rejection is not a major confounding factor and Cyclosporin A (CsA) treatment does not prevent the development of CAV, similar to what we find in the clinical setting. A7-day period of CsA is required in this model to prevent acute rejection and to achieve long-term survival with the development of TVP. This model can also be used to investigate acute cellular rejection and media necrosis in xenogeneic models.
AB - Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant vasculopathy (TVP). The commonly used animal model for cardiovascular chronic rejection studies is the heterotopic heart transplant model performed in laboratory rodents. This model is used widely in experiments since Ono and Lindsey published their technique. To analyze the findings in the blood vessels, the heart has to be sectioned and all vessels have to be measured. Another method to investigate chronic rejection in cardiovascular questionings is the aortic transplant model. In the orthotopic aortic transplant model, the aorta can easily be histologically evaluated. The PVG-to-ACI model is especially useful for CAV studies, since acute vascular rejection is not a major confounding factor and Cyclosporin A (CsA) treatment does not prevent the development of CAV, similar to what we find in the clinical setting. A7-day period of CsA is required in this model to prevent acute rejection and to achieve long-term survival with the development of TVP. This model can also be used to investigate acute cellular rejection and media necrosis in xenogeneic models.
KW - Animals
KW - Aorta/transplantation
KW - Chronic Disease
KW - Disease Models, Animal
KW - Graft Rejection/pathology
KW - Rats
KW - Rats, Inbred ACI
KW - Transplantation, Homologous/adverse effects
KW - Vascular Diseases/etiology
U2 - 10.3791/1989
DO - 10.3791/1989
M3 - SCORING: Journal article
C2 - 21178963
JO - JOVE-J VIS EXP
JF - JOVE-J VIS EXP
SN - 1940-087X
IS - 46
ER -