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Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential

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Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential. / Schlüter, Kerstin; Gassmann, Peter; Enns, Andreas; Korb, Timo; Hemping-Bovenkerk, Andre; Hölzen, Jens; Haier, Jörg.

In: AM J PATHOL, Vol. 169, No. 3, 09.2006, p. 1064-73.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schlüter, K, Gassmann, P, Enns, A, Korb, T, Hemping-Bovenkerk, A, Hölzen, J & Haier, J 2006, 'Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential', AM J PATHOL, vol. 169, no. 3, pp. 1064-73. https://doi.org/10.2353/ajpath.2006.050566

APA

Schlüter, K., Gassmann, P., Enns, A., Korb, T., Hemping-Bovenkerk, A., Hölzen, J., & Haier, J. (2006). Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential. AM J PATHOL, 169(3), 1064-73. https://doi.org/10.2353/ajpath.2006.050566

Vancouver

Schlüter K, Gassmann P, Enns A, Korb T, Hemping-Bovenkerk A, Hölzen J et al. Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential. AM J PATHOL. 2006 Sep;169(3):1064-73. https://doi.org/10.2353/ajpath.2006.050566

Bibtex

@article{f04d775739984ca9b4fac0dcbcc71d0d,
title = "Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential",
abstract = "Adhesive and invasive characteristics appear to be crucial for organ-specific metastasis formation. Using intravital microscopy we investigated the relation between the metastatic potential of colon carcinoma cells and their adhesive and invasive behavior during early steps of metastasis within microvasculatures of rat liver, lung, intestine, skin, muscle, spleen, and kidney in vivo. Colon carcinoma cells with low (HT-29P), intermediate (KM-12C), and high (HT-29LMM, KM-12L4) metastatic potential were injected into nude or Sprague-Dawley rats. Initial interactions with host organ microvasculatures were semiquantitatively analyzed throughout 20 to 30 minutes. Circulating cells passed microvessels in all observed organs without size restriction. All cell lines showed high adhesion rates, independent from their metastatic potential, within liver and lung but very rarely in other organs. Diameters of involved microvessels were larger than diameters of adherent tumor cells. Cell extravasation of highly metastatic HT-29LMM and KM-12L4 cells into liver parenchyma was significantly higher compared to low metastatic cells (P<0.05). Our results indicate that colon carcinoma cells can arrest in target organs without size restriction. Cell adhesion of circulating tumor cells occurred in metastatic target organs only, likely attributable to specific interactions. Migration into target organs correlated with their metastatic potential.",
keywords = "Animals, Carcinoma, Cell Adhesion, Colonic Neoplasms, Liver Neoplasms, Lung Neoplasms, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasms, Experimental, Organ Specificity, Rats, Rats, Nude, Rats, Sprague-Dawley",
author = "Kerstin Schl{\"u}ter and Peter Gassmann and Andreas Enns and Timo Korb and Andre Hemping-Bovenkerk and Jens H{\"o}lzen and J{\"o}rg Haier",
year = "2006",
month = sep,
doi = "10.2353/ajpath.2006.050566",
language = "English",
volume = "169",
pages = "1064--73",
journal = "AM J PATHOL",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential

AU - Schlüter, Kerstin

AU - Gassmann, Peter

AU - Enns, Andreas

AU - Korb, Timo

AU - Hemping-Bovenkerk, Andre

AU - Hölzen, Jens

AU - Haier, Jörg

PY - 2006/9

Y1 - 2006/9

N2 - Adhesive and invasive characteristics appear to be crucial for organ-specific metastasis formation. Using intravital microscopy we investigated the relation between the metastatic potential of colon carcinoma cells and their adhesive and invasive behavior during early steps of metastasis within microvasculatures of rat liver, lung, intestine, skin, muscle, spleen, and kidney in vivo. Colon carcinoma cells with low (HT-29P), intermediate (KM-12C), and high (HT-29LMM, KM-12L4) metastatic potential were injected into nude or Sprague-Dawley rats. Initial interactions with host organ microvasculatures were semiquantitatively analyzed throughout 20 to 30 minutes. Circulating cells passed microvessels in all observed organs without size restriction. All cell lines showed high adhesion rates, independent from their metastatic potential, within liver and lung but very rarely in other organs. Diameters of involved microvessels were larger than diameters of adherent tumor cells. Cell extravasation of highly metastatic HT-29LMM and KM-12L4 cells into liver parenchyma was significantly higher compared to low metastatic cells (P<0.05). Our results indicate that colon carcinoma cells can arrest in target organs without size restriction. Cell adhesion of circulating tumor cells occurred in metastatic target organs only, likely attributable to specific interactions. Migration into target organs correlated with their metastatic potential.

AB - Adhesive and invasive characteristics appear to be crucial for organ-specific metastasis formation. Using intravital microscopy we investigated the relation between the metastatic potential of colon carcinoma cells and their adhesive and invasive behavior during early steps of metastasis within microvasculatures of rat liver, lung, intestine, skin, muscle, spleen, and kidney in vivo. Colon carcinoma cells with low (HT-29P), intermediate (KM-12C), and high (HT-29LMM, KM-12L4) metastatic potential were injected into nude or Sprague-Dawley rats. Initial interactions with host organ microvasculatures were semiquantitatively analyzed throughout 20 to 30 minutes. Circulating cells passed microvessels in all observed organs without size restriction. All cell lines showed high adhesion rates, independent from their metastatic potential, within liver and lung but very rarely in other organs. Diameters of involved microvessels were larger than diameters of adherent tumor cells. Cell extravasation of highly metastatic HT-29LMM and KM-12L4 cells into liver parenchyma was significantly higher compared to low metastatic cells (P<0.05). Our results indicate that colon carcinoma cells can arrest in target organs without size restriction. Cell adhesion of circulating tumor cells occurred in metastatic target organs only, likely attributable to specific interactions. Migration into target organs correlated with their metastatic potential.

KW - Animals

KW - Carcinoma

KW - Cell Adhesion

KW - Colonic Neoplasms

KW - Liver Neoplasms

KW - Lung Neoplasms

KW - Neoplasm Invasiveness

KW - Neoplasm Metastasis

KW - Neoplasms, Experimental

KW - Organ Specificity

KW - Rats

KW - Rats, Nude

KW - Rats, Sprague-Dawley

U2 - 10.2353/ajpath.2006.050566

DO - 10.2353/ajpath.2006.050566

M3 - SCORING: Journal article

C2 - 16936278

VL - 169

SP - 1064

EP - 1073

JO - AM J PATHOL

JF - AM J PATHOL

SN - 0002-9440

IS - 3

ER -