Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential
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Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential. / Schlüter, Kerstin; Gassmann, Peter; Enns, Andreas; Korb, Timo; Hemping-Bovenkerk, Andre; Hölzen, Jens; Haier, Jörg.
in: AM J PATHOL, Jahrgang 169, Nr. 3, 09.2006, S. 1064-73.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential
AU - Schlüter, Kerstin
AU - Gassmann, Peter
AU - Enns, Andreas
AU - Korb, Timo
AU - Hemping-Bovenkerk, Andre
AU - Hölzen, Jens
AU - Haier, Jörg
PY - 2006/9
Y1 - 2006/9
N2 - Adhesive and invasive characteristics appear to be crucial for organ-specific metastasis formation. Using intravital microscopy we investigated the relation between the metastatic potential of colon carcinoma cells and their adhesive and invasive behavior during early steps of metastasis within microvasculatures of rat liver, lung, intestine, skin, muscle, spleen, and kidney in vivo. Colon carcinoma cells with low (HT-29P), intermediate (KM-12C), and high (HT-29LMM, KM-12L4) metastatic potential were injected into nude or Sprague-Dawley rats. Initial interactions with host organ microvasculatures were semiquantitatively analyzed throughout 20 to 30 minutes. Circulating cells passed microvessels in all observed organs without size restriction. All cell lines showed high adhesion rates, independent from their metastatic potential, within liver and lung but very rarely in other organs. Diameters of involved microvessels were larger than diameters of adherent tumor cells. Cell extravasation of highly metastatic HT-29LMM and KM-12L4 cells into liver parenchyma was significantly higher compared to low metastatic cells (P<0.05). Our results indicate that colon carcinoma cells can arrest in target organs without size restriction. Cell adhesion of circulating tumor cells occurred in metastatic target organs only, likely attributable to specific interactions. Migration into target organs correlated with their metastatic potential.
AB - Adhesive and invasive characteristics appear to be crucial for organ-specific metastasis formation. Using intravital microscopy we investigated the relation between the metastatic potential of colon carcinoma cells and their adhesive and invasive behavior during early steps of metastasis within microvasculatures of rat liver, lung, intestine, skin, muscle, spleen, and kidney in vivo. Colon carcinoma cells with low (HT-29P), intermediate (KM-12C), and high (HT-29LMM, KM-12L4) metastatic potential were injected into nude or Sprague-Dawley rats. Initial interactions with host organ microvasculatures were semiquantitatively analyzed throughout 20 to 30 minutes. Circulating cells passed microvessels in all observed organs without size restriction. All cell lines showed high adhesion rates, independent from their metastatic potential, within liver and lung but very rarely in other organs. Diameters of involved microvessels were larger than diameters of adherent tumor cells. Cell extravasation of highly metastatic HT-29LMM and KM-12L4 cells into liver parenchyma was significantly higher compared to low metastatic cells (P<0.05). Our results indicate that colon carcinoma cells can arrest in target organs without size restriction. Cell adhesion of circulating tumor cells occurred in metastatic target organs only, likely attributable to specific interactions. Migration into target organs correlated with their metastatic potential.
KW - Animals
KW - Carcinoma
KW - Cell Adhesion
KW - Colonic Neoplasms
KW - Liver Neoplasms
KW - Lung Neoplasms
KW - Neoplasm Invasiveness
KW - Neoplasm Metastasis
KW - Neoplasms, Experimental
KW - Organ Specificity
KW - Rats
KW - Rats, Nude
KW - Rats, Sprague-Dawley
U2 - 10.2353/ajpath.2006.050566
DO - 10.2353/ajpath.2006.050566
M3 - SCORING: Journal article
C2 - 16936278
VL - 169
SP - 1064
EP - 1073
JO - AM J PATHOL
JF - AM J PATHOL
SN - 0002-9440
IS - 3
ER -