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Organ-specific CD4+ T cell response during Listeria monocytogenes infection

Standard

Organ-specific CD4+ T cell response during Listeria monocytogenes infection. / Kursar, Mischo; Bonhagen, Kerstin; Köhler, Anne; Kamradt, Thomas; Kaufmann, Stefan H E; Mittrücker, Hans-Willi.

In: J IMMUNOL, Vol. 168, No. 12, 15.06.2002, p. 6382-7.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kursar, M, Bonhagen, K, Köhler, A, Kamradt, T, Kaufmann, SHE & Mittrücker, H-W 2002, 'Organ-specific CD4+ T cell response during Listeria monocytogenes infection', J IMMUNOL, vol. 168, no. 12, pp. 6382-7.

APA

Kursar, M., Bonhagen, K., Köhler, A., Kamradt, T., Kaufmann, S. H. E., & Mittrücker, H-W. (2002). Organ-specific CD4+ T cell response during Listeria monocytogenes infection. J IMMUNOL, 168(12), 6382-7.

Vancouver

Kursar M, Bonhagen K, Köhler A, Kamradt T, Kaufmann SHE, Mittrücker H-W. Organ-specific CD4+ T cell response during Listeria monocytogenes infection. J IMMUNOL. 2002 Jun 15;168(12):6382-7.

Bibtex

@article{15597700f331401c8421df38328f4e39,
title = "Organ-specific CD4+ T cell response during Listeria monocytogenes infection",
abstract = "The immune response against the intracellular bacterium Listeria monocytogenes involves both CD4(+) and CD8(+) T cells. We used the MHC class II-presented peptide listeriolysin(189-201) to characterize the organ-specific CD4(+) T cell response during infection. Systemic listeriosis resulted in a strong peptide-specific CD4(+) T cell response with frequencies of 1/100 and 1/30 CD4(+) splenocytes at the peak of primary and secondary response, respectively. This response was not restricted to lymphoid organs, because we detected specific CD4(+) T cells in all tissues analyzed. However, the tissue distribution of the T cell response was dependent on the route of infection. After i.v. infection, the strongest CD4(+) T cell response and the highest levels of memory cells were observed in spleen and liver, the major sites of L. monocytogenes replication. After oral infection, we detected a strong response in the liver, the lamina propria, and the intestinal epithelium. These tissues also harbored the highest frequencies of listeriolysin(189-201)-specific CD4(+) memory T cells 5-8 wk post oral infection. Our results show that kinetics and magnitude of the CD4(+) T cell response and the accumulation of CD4(+) memory T cells depend on the route of infection and are regulated in a tissue-specific way.",
keywords = "Administration, Oral, Animals, Antigens, Bacterial, Bacterial Toxins, CD4-Positive T-Lymphocytes, Cells, Cultured, Cytokines, Epitopes, T-Lymphocyte, Heat-Shock Proteins, Hemolysin Proteins, Immunologic Memory, Injections, Intravenous, Interferon-gamma, Intestine, Small, Intubation, Gastrointestinal, Kinetics, Listeria monocytogenes, Listeriosis, Liver, Mice, Mice, Inbred C57BL, Organ Specificity, Spleen, Tumor Necrosis Factor-alpha",
author = "Mischo Kursar and Kerstin Bonhagen and Anne K{\"o}hler and Thomas Kamradt and Kaufmann, {Stefan H E} and Hans-Willi Mittr{\"u}cker",
year = "2002",
month = jun,
day = "15",
language = "English",
volume = "168",
pages = "6382--7",
journal = "J IMMUNOL",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

RIS

TY - JOUR

T1 - Organ-specific CD4+ T cell response during Listeria monocytogenes infection

AU - Kursar, Mischo

AU - Bonhagen, Kerstin

AU - Köhler, Anne

AU - Kamradt, Thomas

AU - Kaufmann, Stefan H E

AU - Mittrücker, Hans-Willi

PY - 2002/6/15

Y1 - 2002/6/15

N2 - The immune response against the intracellular bacterium Listeria monocytogenes involves both CD4(+) and CD8(+) T cells. We used the MHC class II-presented peptide listeriolysin(189-201) to characterize the organ-specific CD4(+) T cell response during infection. Systemic listeriosis resulted in a strong peptide-specific CD4(+) T cell response with frequencies of 1/100 and 1/30 CD4(+) splenocytes at the peak of primary and secondary response, respectively. This response was not restricted to lymphoid organs, because we detected specific CD4(+) T cells in all tissues analyzed. However, the tissue distribution of the T cell response was dependent on the route of infection. After i.v. infection, the strongest CD4(+) T cell response and the highest levels of memory cells were observed in spleen and liver, the major sites of L. monocytogenes replication. After oral infection, we detected a strong response in the liver, the lamina propria, and the intestinal epithelium. These tissues also harbored the highest frequencies of listeriolysin(189-201)-specific CD4(+) memory T cells 5-8 wk post oral infection. Our results show that kinetics and magnitude of the CD4(+) T cell response and the accumulation of CD4(+) memory T cells depend on the route of infection and are regulated in a tissue-specific way.

AB - The immune response against the intracellular bacterium Listeria monocytogenes involves both CD4(+) and CD8(+) T cells. We used the MHC class II-presented peptide listeriolysin(189-201) to characterize the organ-specific CD4(+) T cell response during infection. Systemic listeriosis resulted in a strong peptide-specific CD4(+) T cell response with frequencies of 1/100 and 1/30 CD4(+) splenocytes at the peak of primary and secondary response, respectively. This response was not restricted to lymphoid organs, because we detected specific CD4(+) T cells in all tissues analyzed. However, the tissue distribution of the T cell response was dependent on the route of infection. After i.v. infection, the strongest CD4(+) T cell response and the highest levels of memory cells were observed in spleen and liver, the major sites of L. monocytogenes replication. After oral infection, we detected a strong response in the liver, the lamina propria, and the intestinal epithelium. These tissues also harbored the highest frequencies of listeriolysin(189-201)-specific CD4(+) memory T cells 5-8 wk post oral infection. Our results show that kinetics and magnitude of the CD4(+) T cell response and the accumulation of CD4(+) memory T cells depend on the route of infection and are regulated in a tissue-specific way.

KW - Administration, Oral

KW - Animals

KW - Antigens, Bacterial

KW - Bacterial Toxins

KW - CD4-Positive T-Lymphocytes

KW - Cells, Cultured

KW - Cytokines

KW - Epitopes, T-Lymphocyte

KW - Heat-Shock Proteins

KW - Hemolysin Proteins

KW - Immunologic Memory

KW - Injections, Intravenous

KW - Interferon-gamma

KW - Intestine, Small

KW - Intubation, Gastrointestinal

KW - Kinetics

KW - Listeria monocytogenes

KW - Listeriosis

KW - Liver

KW - Mice

KW - Mice, Inbred C57BL

KW - Organ Specificity

KW - Spleen

KW - Tumor Necrosis Factor-alpha

M3 - SCORING: Journal article

C2 - 12055256

VL - 168

SP - 6382

EP - 6387

JO - J IMMUNOL

JF - J IMMUNOL

SN - 0022-1767

IS - 12

ER -