Organ-specific CD4+ T cell response during Listeria monocytogenes infection
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Organ-specific CD4+ T cell response during Listeria monocytogenes infection. / Kursar, Mischo; Bonhagen, Kerstin; Köhler, Anne; Kamradt, Thomas; Kaufmann, Stefan H E; Mittrücker, Hans-Willi.
in: J IMMUNOL, Jahrgang 168, Nr. 12, 15.06.2002, S. 6382-7.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Organ-specific CD4+ T cell response during Listeria monocytogenes infection
AU - Kursar, Mischo
AU - Bonhagen, Kerstin
AU - Köhler, Anne
AU - Kamradt, Thomas
AU - Kaufmann, Stefan H E
AU - Mittrücker, Hans-Willi
PY - 2002/6/15
Y1 - 2002/6/15
N2 - The immune response against the intracellular bacterium Listeria monocytogenes involves both CD4(+) and CD8(+) T cells. We used the MHC class II-presented peptide listeriolysin(189-201) to characterize the organ-specific CD4(+) T cell response during infection. Systemic listeriosis resulted in a strong peptide-specific CD4(+) T cell response with frequencies of 1/100 and 1/30 CD4(+) splenocytes at the peak of primary and secondary response, respectively. This response was not restricted to lymphoid organs, because we detected specific CD4(+) T cells in all tissues analyzed. However, the tissue distribution of the T cell response was dependent on the route of infection. After i.v. infection, the strongest CD4(+) T cell response and the highest levels of memory cells were observed in spleen and liver, the major sites of L. monocytogenes replication. After oral infection, we detected a strong response in the liver, the lamina propria, and the intestinal epithelium. These tissues also harbored the highest frequencies of listeriolysin(189-201)-specific CD4(+) memory T cells 5-8 wk post oral infection. Our results show that kinetics and magnitude of the CD4(+) T cell response and the accumulation of CD4(+) memory T cells depend on the route of infection and are regulated in a tissue-specific way.
AB - The immune response against the intracellular bacterium Listeria monocytogenes involves both CD4(+) and CD8(+) T cells. We used the MHC class II-presented peptide listeriolysin(189-201) to characterize the organ-specific CD4(+) T cell response during infection. Systemic listeriosis resulted in a strong peptide-specific CD4(+) T cell response with frequencies of 1/100 and 1/30 CD4(+) splenocytes at the peak of primary and secondary response, respectively. This response was not restricted to lymphoid organs, because we detected specific CD4(+) T cells in all tissues analyzed. However, the tissue distribution of the T cell response was dependent on the route of infection. After i.v. infection, the strongest CD4(+) T cell response and the highest levels of memory cells were observed in spleen and liver, the major sites of L. monocytogenes replication. After oral infection, we detected a strong response in the liver, the lamina propria, and the intestinal epithelium. These tissues also harbored the highest frequencies of listeriolysin(189-201)-specific CD4(+) memory T cells 5-8 wk post oral infection. Our results show that kinetics and magnitude of the CD4(+) T cell response and the accumulation of CD4(+) memory T cells depend on the route of infection and are regulated in a tissue-specific way.
KW - Administration, Oral
KW - Animals
KW - Antigens, Bacterial
KW - Bacterial Toxins
KW - CD4-Positive T-Lymphocytes
KW - Cells, Cultured
KW - Cytokines
KW - Epitopes, T-Lymphocyte
KW - Heat-Shock Proteins
KW - Hemolysin Proteins
KW - Immunologic Memory
KW - Injections, Intravenous
KW - Interferon-gamma
KW - Intestine, Small
KW - Intubation, Gastrointestinal
KW - Kinetics
KW - Listeria monocytogenes
KW - Listeriosis
KW - Liver
KW - Mice
KW - Mice, Inbred C57BL
KW - Organ Specificity
KW - Spleen
KW - Tumor Necrosis Factor-alpha
M3 - SCORING: Journal article
C2 - 12055256
VL - 168
SP - 6382
EP - 6387
JO - J IMMUNOL
JF - J IMMUNOL
SN - 0022-1767
IS - 12
ER -