Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy
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Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy. / Matthies, Levi; Gebrekidan, Medhanie Tesfay; Tegtmeyer, Jasper F.; Oetter, Nicolai; Rohde, Maximilian; Vollkommer, Tobias; Smeets, Ralf; Wilczak, Waldemar; Stelzle, Florian; Gosau, Martin; Braeuer, Andreas Siegfried; Knipfer, Christian.
In: BIOMED OPT EXPRESS, Vol. 12, No. 2, 01.02.2021, p. 836-851.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy
AU - Matthies, Levi
AU - Gebrekidan, Medhanie Tesfay
AU - Tegtmeyer, Jasper F.
AU - Oetter, Nicolai
AU - Rohde, Maximilian
AU - Vollkommer, Tobias
AU - Smeets, Ralf
AU - Wilczak, Waldemar
AU - Stelzle, Florian
AU - Gosau, Martin
AU - Braeuer, Andreas Siegfried
AU - Knipfer, Christian
PY - 2021/2/1
Y1 - 2021/2/1
N2 - Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers and frequently preceded by non-malignant lesions. Using Shifted-Excitation Raman Difference Spectroscopy (SERDS), principal component and linear discriminant analysis in native tissue specimens, 9500 raw Raman spectra of OSCC, 4300 of non-malignant lesions and 4200 of physiological mucosa were evaluated. Non-malignant lesions were distinguished from physiological mucosa with a classification accuracy of 95.3% (95.4% sensitivity, 95.2% specificity, area under the curve (AUC) 0.99). Discriminating OSCC from non-malignant lesions showed an accuracy of 88.4% (93.7% sensitivity, 76.7% specificity, AUC 0.93). OSCC was identified against physiological mucosa with an accuracy of 89.8% (93.7% sensitivity, 81.0% specificity, AUC 0.90). These findings underline the potential of SERDS for the diagnosis of oral cavity lesions.
AB - Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers and frequently preceded by non-malignant lesions. Using Shifted-Excitation Raman Difference Spectroscopy (SERDS), principal component and linear discriminant analysis in native tissue specimens, 9500 raw Raman spectra of OSCC, 4300 of non-malignant lesions and 4200 of physiological mucosa were evaluated. Non-malignant lesions were distinguished from physiological mucosa with a classification accuracy of 95.3% (95.4% sensitivity, 95.2% specificity, area under the curve (AUC) 0.99). Discriminating OSCC from non-malignant lesions showed an accuracy of 88.4% (93.7% sensitivity, 76.7% specificity, AUC 0.93). OSCC was identified against physiological mucosa with an accuracy of 89.8% (93.7% sensitivity, 81.0% specificity, AUC 0.90). These findings underline the potential of SERDS for the diagnosis of oral cavity lesions.
U2 - 10.1364/BOE.409456
DO - 10.1364/BOE.409456
M3 - SCORING: Journal article
VL - 12
SP - 836
EP - 851
JO - BIOMED OPT EXPRESS
JF - BIOMED OPT EXPRESS
SN - 2156-7085
IS - 2
ER -