New technique for chest opening in mice: U-sternotomy

Sonja Schrepfer; Tobias Deuse; Hermann Reichenspurner

doi:10.1002/micr.10114

New technique for chest opening in mice: U-sternotomy

Standard

New technique for chest opening in mice: U-sternotomy. / Schrepfer, Sonja; Deuse, Tobias; Reichenspurner, Hermann.

In: MICROSURG, Vol. 23, No. 3, 2003, p. 274-275.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schrepfer, S, Deuse, T & Reichenspurner, H 2003, 'New technique for chest opening in mice: U-sternotomy', MICROSURG, vol. 23, no. 3, pp. 274-275. https://doi.org/10.1002/micr.10114

APA

Schrepfer, S., Deuse, T., & Reichenspurner, H. (2003). New technique for chest opening in mice: U-sternotomy. MICROSURG, 23(3), 274-275. https://doi.org/10.1002/micr.10114

Vancouver

Schrepfer S, Deuse T, Reichenspurner H. New technique for chest opening in mice: U-sternotomy. MICROSURG. 2003;23(3):274-275. https://doi.org/10.1002/micr.10114

Bibtex

@article{e2b1fd0c65634fe8a63c9585dc1e0383,

title = "New technique for chest opening in mice: U-sternotomy",

abstract = "In cardiac xenotransplantation, transfections with recombinant adeno-associated viruses could be a promising way of modulating the cardiomyocyte genome. Proteins like TRAIL, hDAF, Il-10, Il-4, and beta-galactosidase may be expressed on cardiomyocyte surfaces in order to prevent cellular rejection processes. However, after intracoronary perfusion with transfecting viruses, it takes up to 4 weeks before expression of the transgene can be detected. Mouse models have been established to create reliable transfection protocols. Currently, the major problem involves performing the intraaortic injection in mice without causing a lethal pneumothorax. Here we describe a new technique for chest opening to safely reach the mouse ascending aorta without opening the pleural space. This U-sternotomy reduces the risk of animal death and therefore the amount of quite expensive virus solutions needed.",

keywords = "Animals, Aorta, Heart Transplantation/immunology, Injections, Intra-Arterial/methods, Male, Mice, Models, Animal, Myocytes, Cardiac/immunology, Sternum/surgery, Surgical Procedures, Operative/methods, Transfection/methods, Transplantation, Heterologous/immunology",

author = "Sonja Schrepfer and Tobias Deuse and Hermann Reichenspurner",

year = "2003",

doi = "10.1002/micr.10114",

language = "English",

volume = "23",

pages = "274--275",

journal = "MICROSURG",

issn = "0738-1085",

publisher = "Wiley-Liss Inc.",

number = "3",

}

RIS

TY - JOUR

T1 - New technique for chest opening in mice: U-sternotomy

AU - Schrepfer, Sonja

AU - Deuse, Tobias

AU - Reichenspurner, Hermann

PY - 2003

Y1 - 2003

N2 - In cardiac xenotransplantation, transfections with recombinant adeno-associated viruses could be a promising way of modulating the cardiomyocyte genome. Proteins like TRAIL, hDAF, Il-10, Il-4, and beta-galactosidase may be expressed on cardiomyocyte surfaces in order to prevent cellular rejection processes. However, after intracoronary perfusion with transfecting viruses, it takes up to 4 weeks before expression of the transgene can be detected. Mouse models have been established to create reliable transfection protocols. Currently, the major problem involves performing the intraaortic injection in mice without causing a lethal pneumothorax. Here we describe a new technique for chest opening to safely reach the mouse ascending aorta without opening the pleural space. This U-sternotomy reduces the risk of animal death and therefore the amount of quite expensive virus solutions needed.

AB - In cardiac xenotransplantation, transfections with recombinant adeno-associated viruses could be a promising way of modulating the cardiomyocyte genome. Proteins like TRAIL, hDAF, Il-10, Il-4, and beta-galactosidase may be expressed on cardiomyocyte surfaces in order to prevent cellular rejection processes. However, after intracoronary perfusion with transfecting viruses, it takes up to 4 weeks before expression of the transgene can be detected. Mouse models have been established to create reliable transfection protocols. Currently, the major problem involves performing the intraaortic injection in mice without causing a lethal pneumothorax. Here we describe a new technique for chest opening to safely reach the mouse ascending aorta without opening the pleural space. This U-sternotomy reduces the risk of animal death and therefore the amount of quite expensive virus solutions needed.

KW - Animals

KW - Aorta

KW - Heart Transplantation/immunology

KW - Injections, Intra-Arterial/methods

KW - Male

KW - Mice

KW - Models, Animal

KW - Myocytes, Cardiac/immunology

KW - Sternum/surgery

KW - Surgical Procedures, Operative/methods

KW - Transfection/methods

KW - Transplantation, Heterologous/immunology

U2 - 10.1002/micr.10114

DO - 10.1002/micr.10114

M3 - SCORING: Journal article

C2 - 12833331

VL - 23

SP - 274

EP - 275

JO - MICROSURG

JF - MICROSURG

SN - 0738-1085

IS - 3

ER -