New technique for chest opening in mice: U-sternotomy
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New technique for chest opening in mice: U-sternotomy. / Schrepfer, Sonja; Deuse, Tobias; Reichenspurner, Hermann.
in: MICROSURG, Jahrgang 23, Nr. 3, 2003, S. 274-275.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - New technique for chest opening in mice: U-sternotomy
AU - Schrepfer, Sonja
AU - Deuse, Tobias
AU - Reichenspurner, Hermann
N1 - Copyright 2003 Wiley-Liss, Inc.
PY - 2003
Y1 - 2003
N2 - In cardiac xenotransplantation, transfections with recombinant adeno-associated viruses could be a promising way of modulating the cardiomyocyte genome. Proteins like TRAIL, hDAF, Il-10, Il-4, and beta-galactosidase may be expressed on cardiomyocyte surfaces in order to prevent cellular rejection processes. However, after intracoronary perfusion with transfecting viruses, it takes up to 4 weeks before expression of the transgene can be detected. Mouse models have been established to create reliable transfection protocols. Currently, the major problem involves performing the intraaortic injection in mice without causing a lethal pneumothorax. Here we describe a new technique for chest opening to safely reach the mouse ascending aorta without opening the pleural space. This U-sternotomy reduces the risk of animal death and therefore the amount of quite expensive virus solutions needed.
AB - In cardiac xenotransplantation, transfections with recombinant adeno-associated viruses could be a promising way of modulating the cardiomyocyte genome. Proteins like TRAIL, hDAF, Il-10, Il-4, and beta-galactosidase may be expressed on cardiomyocyte surfaces in order to prevent cellular rejection processes. However, after intracoronary perfusion with transfecting viruses, it takes up to 4 weeks before expression of the transgene can be detected. Mouse models have been established to create reliable transfection protocols. Currently, the major problem involves performing the intraaortic injection in mice without causing a lethal pneumothorax. Here we describe a new technique for chest opening to safely reach the mouse ascending aorta without opening the pleural space. This U-sternotomy reduces the risk of animal death and therefore the amount of quite expensive virus solutions needed.
KW - Animals
KW - Aorta
KW - Heart Transplantation/immunology
KW - Injections, Intra-Arterial/methods
KW - Male
KW - Mice
KW - Models, Animal
KW - Myocytes, Cardiac/immunology
KW - Sternum/surgery
KW - Surgical Procedures, Operative/methods
KW - Transfection/methods
KW - Transplantation, Heterologous/immunology
U2 - 10.1002/micr.10114
DO - 10.1002/micr.10114
M3 - SCORING: Journal article
C2 - 12833331
VL - 23
SP - 274
EP - 275
JO - MICROSURG
JF - MICROSURG
SN - 0738-1085
IS - 3
ER -