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Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe

Standard

Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe. / Kabisch, H; Heinsohn, S; Milde-Langosch, Karin; Löning, T; Bartl, S; Erttmann, R; Landbeck, G.

In: MONATSSCHR KINDERH, Vol. 135, No. 4, 04.1987, p. 210-213.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kabisch, H, Heinsohn, S, Milde-Langosch, K, Löning, T, Bartl, S, Erttmann, R & Landbeck, G 1987, 'Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe', MONATSSCHR KINDERH, vol. 135, no. 4, pp. 210-213. <http://www.ncbi.nlm.nih.gov/pubmed/3600672?dopt=Citation>

APA

Kabisch, H., Heinsohn, S., Milde-Langosch, K., Löning, T., Bartl, S., Erttmann, R., & Landbeck, G. (1987). Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe. MONATSSCHR KINDERH, 135(4), 210-213. http://www.ncbi.nlm.nih.gov/pubmed/3600672?dopt=Citation

Vancouver

Kabisch H, Heinsohn S, Milde-Langosch K, Löning T, Bartl S, Erttmann R et al. Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe. MONATSSCHR KINDERH. 1987 Apr;135(4):210-213.

Bibtex

@article{f820bf7f89a54ff291ec7d771d3d71cd,
title = "Nachweis von Neuroblastomzellen im Knochenmark durch {"}Southern-Blot{"} und {"}In situ-Hybridisierung{"} mit Hilfe einer NMYC-DNS-Probe",
abstract = "Recently an amplification of NMYC oncogene was discovered in stage III and stage IV as well as in stage II neuroblastoma cells. The extent of NMYC amplification is of prognostic value. A high expression of NMYC on mRNA level is correlated to the NMYC gene-amplification. We utilized the in situ-hybridization technique for the detection of NMYC-mRNA expression on cellular level, and the Southern Blot method for analysing the total genomic DNA. In three stage IV patients with overt neuroblastoma in bone marrow cells, NMYC gene-amplification as well as NMYC-mRNA were detected. Seven control bone marrows of patients with different heamatological diseases in different stages did not contain positive cells in our in situ-hybridization assay.",
keywords = "Base Sequence, Biopsy, Needle, Bone Marrow, Cell Transformation, Neoplastic, Child, DNA, Neoplasm, Gene Amplification, Gene Expression Regulation, Humans, Neoplasm Recurrence, Local, Neuroblastoma, Nucleic Acid Hybridization, Oncogenes, Prognosis, RNA, Messenger",
author = "H Kabisch and S Heinsohn and Karin Milde-Langosch and T L{\"o}ning and S Bartl and R Erttmann and G Landbeck",
year = "1987",
month = apr,
language = "Deutsch",
volume = "135",
pages = "210--213",
journal = "MONATSSCHR KINDERH",
issn = "0026-9298",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe

AU - Kabisch, H

AU - Heinsohn, S

AU - Milde-Langosch, Karin

AU - Löning, T

AU - Bartl, S

AU - Erttmann, R

AU - Landbeck, G

PY - 1987/4

Y1 - 1987/4

N2 - Recently an amplification of NMYC oncogene was discovered in stage III and stage IV as well as in stage II neuroblastoma cells. The extent of NMYC amplification is of prognostic value. A high expression of NMYC on mRNA level is correlated to the NMYC gene-amplification. We utilized the in situ-hybridization technique for the detection of NMYC-mRNA expression on cellular level, and the Southern Blot method for analysing the total genomic DNA. In three stage IV patients with overt neuroblastoma in bone marrow cells, NMYC gene-amplification as well as NMYC-mRNA were detected. Seven control bone marrows of patients with different heamatological diseases in different stages did not contain positive cells in our in situ-hybridization assay.

AB - Recently an amplification of NMYC oncogene was discovered in stage III and stage IV as well as in stage II neuroblastoma cells. The extent of NMYC amplification is of prognostic value. A high expression of NMYC on mRNA level is correlated to the NMYC gene-amplification. We utilized the in situ-hybridization technique for the detection of NMYC-mRNA expression on cellular level, and the Southern Blot method for analysing the total genomic DNA. In three stage IV patients with overt neuroblastoma in bone marrow cells, NMYC gene-amplification as well as NMYC-mRNA were detected. Seven control bone marrows of patients with different heamatological diseases in different stages did not contain positive cells in our in situ-hybridization assay.

KW - Base Sequence

KW - Biopsy, Needle

KW - Bone Marrow

KW - Cell Transformation, Neoplastic

KW - Child

KW - DNA, Neoplasm

KW - Gene Amplification

KW - Gene Expression Regulation

KW - Humans

KW - Neoplasm Recurrence, Local

KW - Neuroblastoma

KW - Nucleic Acid Hybridization

KW - Oncogenes

KW - Prognosis

KW - RNA, Messenger

M3 - SCORING: Zeitschriftenaufsatz

C2 - 3600672

VL - 135

SP - 210

EP - 213

JO - MONATSSCHR KINDERH

JF - MONATSSCHR KINDERH

SN - 0026-9298

IS - 4

ER -