Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe
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Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe. / Kabisch, H; Heinsohn, S; Milde-Langosch, Karin; Löning, T; Bartl, S; Erttmann, R; Landbeck, G.
in: MONATSSCHR KINDERH, Jahrgang 135, Nr. 4, 04.1987, S. 210-213.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Nachweis von Neuroblastomzellen im Knochenmark durch "Southern-Blot" und "In situ-Hybridisierung" mit Hilfe einer NMYC-DNS-Probe
AU - Kabisch, H
AU - Heinsohn, S
AU - Milde-Langosch, Karin
AU - Löning, T
AU - Bartl, S
AU - Erttmann, R
AU - Landbeck, G
PY - 1987/4
Y1 - 1987/4
N2 - Recently an amplification of NMYC oncogene was discovered in stage III and stage IV as well as in stage II neuroblastoma cells. The extent of NMYC amplification is of prognostic value. A high expression of NMYC on mRNA level is correlated to the NMYC gene-amplification. We utilized the in situ-hybridization technique for the detection of NMYC-mRNA expression on cellular level, and the Southern Blot method for analysing the total genomic DNA. In three stage IV patients with overt neuroblastoma in bone marrow cells, NMYC gene-amplification as well as NMYC-mRNA were detected. Seven control bone marrows of patients with different heamatological diseases in different stages did not contain positive cells in our in situ-hybridization assay.
AB - Recently an amplification of NMYC oncogene was discovered in stage III and stage IV as well as in stage II neuroblastoma cells. The extent of NMYC amplification is of prognostic value. A high expression of NMYC on mRNA level is correlated to the NMYC gene-amplification. We utilized the in situ-hybridization technique for the detection of NMYC-mRNA expression on cellular level, and the Southern Blot method for analysing the total genomic DNA. In three stage IV patients with overt neuroblastoma in bone marrow cells, NMYC gene-amplification as well as NMYC-mRNA were detected. Seven control bone marrows of patients with different heamatological diseases in different stages did not contain positive cells in our in situ-hybridization assay.
KW - Base Sequence
KW - Biopsy, Needle
KW - Bone Marrow
KW - Cell Transformation, Neoplastic
KW - Child
KW - DNA, Neoplasm
KW - Gene Amplification
KW - Gene Expression Regulation
KW - Humans
KW - Neoplasm Recurrence, Local
KW - Neuroblastoma
KW - Nucleic Acid Hybridization
KW - Oncogenes
KW - Prognosis
KW - RNA, Messenger
M3 - SCORING: Zeitschriftenaufsatz
C2 - 3600672
VL - 135
SP - 210
EP - 213
JO - MONATSSCHR KINDERH
JF - MONATSSCHR KINDERH
SN - 0026-9298
IS - 4
ER -