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Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU.

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Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU. / Soghomonyan, Suren; Hajitou, Amin; Rangel, Roberto; Trepel, Martin; Pasqualini, Renata; Arap, Wadih; Gelovani, Juri G; Alauddin, Mian M.

In: NAT PROTOC, Vol. 2, No. 2, 2, 2007, p. 416-423.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Soghomonyan, S, Hajitou, A, Rangel, R, Trepel, M, Pasqualini, R, Arap, W, Gelovani, JG & Alauddin, MM 2007, 'Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU.', NAT PROTOC, vol. 2, no. 2, 2, pp. 416-423. https://doi.org/10.1038/nprot.2007.49

APA

Soghomonyan, S., Hajitou, A., Rangel, R., Trepel, M., Pasqualini, R., Arap, W., Gelovani, J. G., & Alauddin, M. M. (2007). Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU. NAT PROTOC, 2(2), 416-423. [2]. https://doi.org/10.1038/nprot.2007.49

Vancouver

Soghomonyan S, Hajitou A, Rangel R, Trepel M, Pasqualini R, Arap W et al. Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU. NAT PROTOC. 2007;2(2):416-423. 2. https://doi.org/10.1038/nprot.2007.49

Bibtex

@article{e1bdf7d8247141a0acd782cf7467f85a,
title = "Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU.",
abstract = "Non-invasive imaging of transgene expression requires the appropriate combination of a reporter gene and a reporter probe. [18F]FEAU positron emission tomography (PET) is used for the assessment of herpes simplex virus type-1 thymidine kinase gene expression. Hybrid AAV phage (termed AAVP) can be adapted to transduce mammalian cells by targeting to a specific receptor. We evaluated a targeted AAVP vector using [18F]FEAU PET. This protocol describes [18F]FEAU production and dosing, micro-PET imaging and image analysis. 2-Deoxy-2-trifluoromethanesulfonyl-1,3,5-tri-O-benzoyl-alpha-D-ribofuranose is radio-fluorinated, converted into its 1-bromo derivative and coupled with protected 5-ethyl uracil. The coupled product is hydrolyzed and purified using HPLC. Tumor-bearing animals targeted with either retroviral or AAVP vectors are anesthetized and injected with [18F]FEAU (0.1 mCi per mouse); this is followed 2 h after injection by imaging on a micro-PET. Production of [18F]FEAU requires approximately 3.5 h from the end of bombardment. PET imaging studies require 2-3 h (depending on the number of animals) after synthesis of [18F]FEAU.",
keywords = "Animals, Arabinofuranosyluracil, Cell Line, Tumor, Fluorine Radioisotopes, Gene Expression Profiling, Gene Targeting, Genes, Reporter, Genetic Vectors, Herpesvirus 1, Human, Humans, Mice, Molecular Structure, Positron-Emission Tomography, Thymidine Kinase, Transgenes, Journal Article",
author = "Suren Soghomonyan and Amin Hajitou and Roberto Rangel and Martin Trepel and Renata Pasqualini and Wadih Arap and Gelovani, {Juri G} and Alauddin, {Mian M}",
year = "2007",
doi = "10.1038/nprot.2007.49",
language = "English",
volume = "2",
pages = "416--423",
journal = "NAT PROTOC",
issn = "1754-2189",
publisher = "NATURE PUBLISHING GROUP",
number = "2",

}

RIS

TY - JOUR

T1 - Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU.

AU - Soghomonyan, Suren

AU - Hajitou, Amin

AU - Rangel, Roberto

AU - Trepel, Martin

AU - Pasqualini, Renata

AU - Arap, Wadih

AU - Gelovani, Juri G

AU - Alauddin, Mian M

PY - 2007

Y1 - 2007

N2 - Non-invasive imaging of transgene expression requires the appropriate combination of a reporter gene and a reporter probe. [18F]FEAU positron emission tomography (PET) is used for the assessment of herpes simplex virus type-1 thymidine kinase gene expression. Hybrid AAV phage (termed AAVP) can be adapted to transduce mammalian cells by targeting to a specific receptor. We evaluated a targeted AAVP vector using [18F]FEAU PET. This protocol describes [18F]FEAU production and dosing, micro-PET imaging and image analysis. 2-Deoxy-2-trifluoromethanesulfonyl-1,3,5-tri-O-benzoyl-alpha-D-ribofuranose is radio-fluorinated, converted into its 1-bromo derivative and coupled with protected 5-ethyl uracil. The coupled product is hydrolyzed and purified using HPLC. Tumor-bearing animals targeted with either retroviral or AAVP vectors are anesthetized and injected with [18F]FEAU (0.1 mCi per mouse); this is followed 2 h after injection by imaging on a micro-PET. Production of [18F]FEAU requires approximately 3.5 h from the end of bombardment. PET imaging studies require 2-3 h (depending on the number of animals) after synthesis of [18F]FEAU.

AB - Non-invasive imaging of transgene expression requires the appropriate combination of a reporter gene and a reporter probe. [18F]FEAU positron emission tomography (PET) is used for the assessment of herpes simplex virus type-1 thymidine kinase gene expression. Hybrid AAV phage (termed AAVP) can be adapted to transduce mammalian cells by targeting to a specific receptor. We evaluated a targeted AAVP vector using [18F]FEAU PET. This protocol describes [18F]FEAU production and dosing, micro-PET imaging and image analysis. 2-Deoxy-2-trifluoromethanesulfonyl-1,3,5-tri-O-benzoyl-alpha-D-ribofuranose is radio-fluorinated, converted into its 1-bromo derivative and coupled with protected 5-ethyl uracil. The coupled product is hydrolyzed and purified using HPLC. Tumor-bearing animals targeted with either retroviral or AAVP vectors are anesthetized and injected with [18F]FEAU (0.1 mCi per mouse); this is followed 2 h after injection by imaging on a micro-PET. Production of [18F]FEAU requires approximately 3.5 h from the end of bombardment. PET imaging studies require 2-3 h (depending on the number of animals) after synthesis of [18F]FEAU.

KW - Animals

KW - Arabinofuranosyluracil

KW - Cell Line, Tumor

KW - Fluorine Radioisotopes

KW - Gene Expression Profiling

KW - Gene Targeting

KW - Genes, Reporter

KW - Genetic Vectors

KW - Herpesvirus 1, Human

KW - Humans

KW - Mice

KW - Molecular Structure

KW - Positron-Emission Tomography

KW - Thymidine Kinase

KW - Transgenes

KW - Journal Article

U2 - 10.1038/nprot.2007.49

DO - 10.1038/nprot.2007.49

M3 - SCORING: Journal article

C2 - 17406603

VL - 2

SP - 416

EP - 423

JO - NAT PROTOC

JF - NAT PROTOC

SN - 1754-2189

IS - 2

M1 - 2

ER -