Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU.
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Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU. / Soghomonyan, Suren; Hajitou, Amin; Rangel, Roberto; Trepel, Martin; Pasqualini, Renata; Arap, Wadih; Gelovani, Juri G; Alauddin, Mian M.
in: NAT PROTOC, Jahrgang 2, Nr. 2, 2, 2007, S. 416-423.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Molecular PET imaging of HSV1-tk reporter gene expression using [18F]FEAU.
AU - Soghomonyan, Suren
AU - Hajitou, Amin
AU - Rangel, Roberto
AU - Trepel, Martin
AU - Pasqualini, Renata
AU - Arap, Wadih
AU - Gelovani, Juri G
AU - Alauddin, Mian M
PY - 2007
Y1 - 2007
N2 - Non-invasive imaging of transgene expression requires the appropriate combination of a reporter gene and a reporter probe. [18F]FEAU positron emission tomography (PET) is used for the assessment of herpes simplex virus type-1 thymidine kinase gene expression. Hybrid AAV phage (termed AAVP) can be adapted to transduce mammalian cells by targeting to a specific receptor. We evaluated a targeted AAVP vector using [18F]FEAU PET. This protocol describes [18F]FEAU production and dosing, micro-PET imaging and image analysis. 2-Deoxy-2-trifluoromethanesulfonyl-1,3,5-tri-O-benzoyl-alpha-D-ribofuranose is radio-fluorinated, converted into its 1-bromo derivative and coupled with protected 5-ethyl uracil. The coupled product is hydrolyzed and purified using HPLC. Tumor-bearing animals targeted with either retroviral or AAVP vectors are anesthetized and injected with [18F]FEAU (0.1 mCi per mouse); this is followed 2 h after injection by imaging on a micro-PET. Production of [18F]FEAU requires approximately 3.5 h from the end of bombardment. PET imaging studies require 2-3 h (depending on the number of animals) after synthesis of [18F]FEAU.
AB - Non-invasive imaging of transgene expression requires the appropriate combination of a reporter gene and a reporter probe. [18F]FEAU positron emission tomography (PET) is used for the assessment of herpes simplex virus type-1 thymidine kinase gene expression. Hybrid AAV phage (termed AAVP) can be adapted to transduce mammalian cells by targeting to a specific receptor. We evaluated a targeted AAVP vector using [18F]FEAU PET. This protocol describes [18F]FEAU production and dosing, micro-PET imaging and image analysis. 2-Deoxy-2-trifluoromethanesulfonyl-1,3,5-tri-O-benzoyl-alpha-D-ribofuranose is radio-fluorinated, converted into its 1-bromo derivative and coupled with protected 5-ethyl uracil. The coupled product is hydrolyzed and purified using HPLC. Tumor-bearing animals targeted with either retroviral or AAVP vectors are anesthetized and injected with [18F]FEAU (0.1 mCi per mouse); this is followed 2 h after injection by imaging on a micro-PET. Production of [18F]FEAU requires approximately 3.5 h from the end of bombardment. PET imaging studies require 2-3 h (depending on the number of animals) after synthesis of [18F]FEAU.
KW - Animals
KW - Arabinofuranosyluracil
KW - Cell Line, Tumor
KW - Fluorine Radioisotopes
KW - Gene Expression Profiling
KW - Gene Targeting
KW - Genes, Reporter
KW - Genetic Vectors
KW - Herpesvirus 1, Human
KW - Humans
KW - Mice
KW - Molecular Structure
KW - Positron-Emission Tomography
KW - Thymidine Kinase
KW - Transgenes
KW - Journal Article
U2 - 10.1038/nprot.2007.49
DO - 10.1038/nprot.2007.49
M3 - SCORING: Journal article
C2 - 17406603
VL - 2
SP - 416
EP - 423
JO - NAT PROTOC
JF - NAT PROTOC
SN - 1754-2189
IS - 2
M1 - 2
ER -