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Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects

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Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects. / Huber, Roman; Rostock, M; Goedl, R; Lüdtke, R; Urech, K; Buck, S; Klein, R.

In: EUR J MED RES, Vol. 10, No. 10, 18.10.2005, p. 411-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Huber, R, Rostock, M, Goedl, R, Lüdtke, R, Urech, K, Buck, S & Klein, R 2005, 'Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects', EUR J MED RES, vol. 10, no. 10, pp. 411-8.

APA

Huber, R., Rostock, M., Goedl, R., Lüdtke, R., Urech, K., Buck, S., & Klein, R. (2005). Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects. EUR J MED RES, 10(10), 411-8.

Vancouver

Huber R, Rostock M, Goedl R, Lüdtke R, Urech K, Buck S et al. Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects. EUR J MED RES. 2005 Oct 18;10(10):411-8.

Bibtex

@article{4937151e2515411a8915a9ff52f06716,
title = "Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects",
abstract = "OBJECTIVE: Various immunological effects have been reported during application of mistletoe preparations. Because these data are heterogeneous, we performed a placebo controlled study to investigate (1) effects on peripheral granulocyte and eosinophil counts, (2) related cytokine levels and (3) whether effects are related to mistletoe lectin (ML).METHODS: 43 volunteers were randomized to receive the mistletoe plant extract Iscador Quercus spezial (IQ), purified ML, IQ which was depleted from ML, or placebo subcutaneously twice per week for 8 weeks. Weekly, differential blood count and every four weeks spontaneous and IQ- and ML-induced cytokine production by peripheral blood mononuclear cells (PBMC) were analyzed.RESULTS: Leukocyte-, granulocyte-, and eosinophil counts were significantly higher during treatment in the IQ- and ML-groups than in the placebo group. Furthermore, a significant increase of antigen-induced production of GM-CSF, IL-5 and IFNgamma by PBMC was observed in the IQ- and ML-group but not in the groups receiving ML-depleted IQ or placebo. Severe side effects did not occur in any of the subjects.CONCLUSIONS: Treatment with IQ or ML stimulates the production of GM-CSF, IL-5 and IFNgamma by PBMC, and this is accompanied by an increase of eosinophil- and granulocyte-counts. These observations may, therefore, open rational therapeutic indications for mistletoe extracts.",
keywords = "Adult, Double-Blind Method, Eosinophils/drug effects, Female, Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis, Granulocytes/drug effects, Humans, In Vitro Techniques, Interleukin-5/biosynthesis, Leukocyte Count, Leukocytes, Mononuclear/drug effects, Male, Phytotherapy, Plant Extracts/pharmacology, Plant Proteins/pharmacology, Prospective Studies, Viscum album",
author = "Roman Huber and M Rostock and R Goedl and R L{\"u}dtke and K Urech and S Buck and R Klein",
year = "2005",
month = oct,
day = "18",
language = "English",
volume = "10",
pages = "411--8",
journal = "EUR J MED RES",
issn = "0949-2321",
publisher = "BioMed Central Ltd.",
number = "10",

}

RIS

TY - JOUR

T1 - Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects

AU - Huber, Roman

AU - Rostock, M

AU - Goedl, R

AU - Lüdtke, R

AU - Urech, K

AU - Buck, S

AU - Klein, R

PY - 2005/10/18

Y1 - 2005/10/18

N2 - OBJECTIVE: Various immunological effects have been reported during application of mistletoe preparations. Because these data are heterogeneous, we performed a placebo controlled study to investigate (1) effects on peripheral granulocyte and eosinophil counts, (2) related cytokine levels and (3) whether effects are related to mistletoe lectin (ML).METHODS: 43 volunteers were randomized to receive the mistletoe plant extract Iscador Quercus spezial (IQ), purified ML, IQ which was depleted from ML, or placebo subcutaneously twice per week for 8 weeks. Weekly, differential blood count and every four weeks spontaneous and IQ- and ML-induced cytokine production by peripheral blood mononuclear cells (PBMC) were analyzed.RESULTS: Leukocyte-, granulocyte-, and eosinophil counts were significantly higher during treatment in the IQ- and ML-groups than in the placebo group. Furthermore, a significant increase of antigen-induced production of GM-CSF, IL-5 and IFNgamma by PBMC was observed in the IQ- and ML-group but not in the groups receiving ML-depleted IQ or placebo. Severe side effects did not occur in any of the subjects.CONCLUSIONS: Treatment with IQ or ML stimulates the production of GM-CSF, IL-5 and IFNgamma by PBMC, and this is accompanied by an increase of eosinophil- and granulocyte-counts. These observations may, therefore, open rational therapeutic indications for mistletoe extracts.

AB - OBJECTIVE: Various immunological effects have been reported during application of mistletoe preparations. Because these data are heterogeneous, we performed a placebo controlled study to investigate (1) effects on peripheral granulocyte and eosinophil counts, (2) related cytokine levels and (3) whether effects are related to mistletoe lectin (ML).METHODS: 43 volunteers were randomized to receive the mistletoe plant extract Iscador Quercus spezial (IQ), purified ML, IQ which was depleted from ML, or placebo subcutaneously twice per week for 8 weeks. Weekly, differential blood count and every four weeks spontaneous and IQ- and ML-induced cytokine production by peripheral blood mononuclear cells (PBMC) were analyzed.RESULTS: Leukocyte-, granulocyte-, and eosinophil counts were significantly higher during treatment in the IQ- and ML-groups than in the placebo group. Furthermore, a significant increase of antigen-induced production of GM-CSF, IL-5 and IFNgamma by PBMC was observed in the IQ- and ML-group but not in the groups receiving ML-depleted IQ or placebo. Severe side effects did not occur in any of the subjects.CONCLUSIONS: Treatment with IQ or ML stimulates the production of GM-CSF, IL-5 and IFNgamma by PBMC, and this is accompanied by an increase of eosinophil- and granulocyte-counts. These observations may, therefore, open rational therapeutic indications for mistletoe extracts.

KW - Adult

KW - Double-Blind Method

KW - Eosinophils/drug effects

KW - Female

KW - Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis

KW - Granulocytes/drug effects

KW - Humans

KW - In Vitro Techniques

KW - Interleukin-5/biosynthesis

KW - Leukocyte Count

KW - Leukocytes, Mononuclear/drug effects

KW - Male

KW - Phytotherapy

KW - Plant Extracts/pharmacology

KW - Plant Proteins/pharmacology

KW - Prospective Studies

KW - Viscum album

M3 - SCORING: Journal article

C2 - 16287602

VL - 10

SP - 411

EP - 418

JO - EUR J MED RES

JF - EUR J MED RES

SN - 0949-2321

IS - 10

ER -