Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects
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Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects. / Huber, Roman; Rostock, M; Goedl, R; Lüdtke, R; Urech, K; Buck, S; Klein, R.
in: EUR J MED RES, Jahrgang 10, Nr. 10, 18.10.2005, S. 411-8.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Mistletoe treatment induces GM-CSF- and IL-5 production by PBMC and increases blood granulocyte- and eosinophil counts - a placebo controlled randomized study in healthy subjects
AU - Huber, Roman
AU - Rostock, M
AU - Goedl, R
AU - Lüdtke, R
AU - Urech, K
AU - Buck, S
AU - Klein, R
PY - 2005/10/18
Y1 - 2005/10/18
N2 - OBJECTIVE: Various immunological effects have been reported during application of mistletoe preparations. Because these data are heterogeneous, we performed a placebo controlled study to investigate (1) effects on peripheral granulocyte and eosinophil counts, (2) related cytokine levels and (3) whether effects are related to mistletoe lectin (ML).METHODS: 43 volunteers were randomized to receive the mistletoe plant extract Iscador Quercus spezial (IQ), purified ML, IQ which was depleted from ML, or placebo subcutaneously twice per week for 8 weeks. Weekly, differential blood count and every four weeks spontaneous and IQ- and ML-induced cytokine production by peripheral blood mononuclear cells (PBMC) were analyzed.RESULTS: Leukocyte-, granulocyte-, and eosinophil counts were significantly higher during treatment in the IQ- and ML-groups than in the placebo group. Furthermore, a significant increase of antigen-induced production of GM-CSF, IL-5 and IFNgamma by PBMC was observed in the IQ- and ML-group but not in the groups receiving ML-depleted IQ or placebo. Severe side effects did not occur in any of the subjects.CONCLUSIONS: Treatment with IQ or ML stimulates the production of GM-CSF, IL-5 and IFNgamma by PBMC, and this is accompanied by an increase of eosinophil- and granulocyte-counts. These observations may, therefore, open rational therapeutic indications for mistletoe extracts.
AB - OBJECTIVE: Various immunological effects have been reported during application of mistletoe preparations. Because these data are heterogeneous, we performed a placebo controlled study to investigate (1) effects on peripheral granulocyte and eosinophil counts, (2) related cytokine levels and (3) whether effects are related to mistletoe lectin (ML).METHODS: 43 volunteers were randomized to receive the mistletoe plant extract Iscador Quercus spezial (IQ), purified ML, IQ which was depleted from ML, or placebo subcutaneously twice per week for 8 weeks. Weekly, differential blood count and every four weeks spontaneous and IQ- and ML-induced cytokine production by peripheral blood mononuclear cells (PBMC) were analyzed.RESULTS: Leukocyte-, granulocyte-, and eosinophil counts were significantly higher during treatment in the IQ- and ML-groups than in the placebo group. Furthermore, a significant increase of antigen-induced production of GM-CSF, IL-5 and IFNgamma by PBMC was observed in the IQ- and ML-group but not in the groups receiving ML-depleted IQ or placebo. Severe side effects did not occur in any of the subjects.CONCLUSIONS: Treatment with IQ or ML stimulates the production of GM-CSF, IL-5 and IFNgamma by PBMC, and this is accompanied by an increase of eosinophil- and granulocyte-counts. These observations may, therefore, open rational therapeutic indications for mistletoe extracts.
KW - Adult
KW - Double-Blind Method
KW - Eosinophils/drug effects
KW - Female
KW - Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis
KW - Granulocytes/drug effects
KW - Humans
KW - In Vitro Techniques
KW - Interleukin-5/biosynthesis
KW - Leukocyte Count
KW - Leukocytes, Mononuclear/drug effects
KW - Male
KW - Phytotherapy
KW - Plant Extracts/pharmacology
KW - Plant Proteins/pharmacology
KW - Prospective Studies
KW - Viscum album
M3 - SCORING: Journal article
C2 - 16287602
VL - 10
SP - 411
EP - 418
JO - EUR J MED RES
JF - EUR J MED RES
SN - 0949-2321
IS - 10
ER -