MicroRNA-9 controls dendritic development by targeting REST
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MicroRNA-9 controls dendritic development by targeting REST. / Giusti, Sebastian A; Vogl, Annette M; Brockmann, Marisa M; Vercelli, Claudia A; Rein, Martin L; Trümbach, Dietrich; Wurst, Wolfgang; Cazalla, Demian; Stein, Valentin; Deussing, Jan M; Refojo, Damian.
In: ELIFE, Vol. 3, 18.11.2014.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - MicroRNA-9 controls dendritic development by targeting REST
AU - Giusti, Sebastian A
AU - Vogl, Annette M
AU - Brockmann, Marisa M
AU - Vercelli, Claudia A
AU - Rein, Martin L
AU - Trümbach, Dietrich
AU - Wurst, Wolfgang
AU - Cazalla, Demian
AU - Stein, Valentin
AU - Deussing, Jan M
AU - Refojo, Damian
PY - 2014/11/18
Y1 - 2014/11/18
N2 - MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.
AB - MicroRNAs (miRNAs) are conserved noncoding RNAs that function as posttranscriptional regulators of gene expression. miR-9 is one of the most abundant miRNAs in the brain. Although the function of miR-9 has been well characterized in neural progenitors, its role in dendritic and synaptic development remains largely unknown. In order to target miR-9 in vivo, we developed a transgenic miRNA sponge mouse line allowing conditional inactivation of the miR-9 family in a spatio-temporal-controlled manner. Using this novel approach, we found that miR-9 controls dendritic growth and synaptic transmission in vivo. Furthermore, we demonstrate that miR-9-mediated downregulation of the transcriptional repressor REST is essential for proper dendritic growth.
KW - Aging/metabolism
KW - Animals
KW - Brain/metabolism
KW - Cells, Cultured
KW - Dendrites/metabolism
KW - Genes, Reporter
KW - HEK293 Cells
KW - Humans
KW - Integrases/metabolism
KW - Mice, Transgenic
KW - MicroRNAs/genetics
KW - Nestin/metabolism
KW - Neurons/metabolism
KW - Repressor Proteins/metabolism
KW - Synaptic Transmission
U2 - 10.7554/eLife.02755
DO - 10.7554/eLife.02755
M3 - SCORING: Journal article
C2 - 25406064
VL - 3
JO - ELIFE
JF - ELIFE
SN - 2050-084X
ER -