Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator
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Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator. / Ferrer, I; Puig, B; Goutan, E; Gombau, L; Muñoz-Cánoves, P; Puig Martorell, Berta.
In: NEUROSCI LETT, Vol. 299, No. 1-2, 16.02.2001, p. 77-80.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator
AU - Ferrer, I
AU - Puig, B
AU - Goutan, E
AU - Gombau, L
AU - Muñoz-Cánoves, P
AU - Puig Martorell, Berta
PY - 2001/2/16
Y1 - 2001/2/16
N2 - Methylazoximethanol (MAM) acetate-induced cell death in the external granule cell layer of the developing cerebellum affects clusters of cells with morphological features of apoptosis. This is accompanied by selective induction of active caspase-3 expression and increased c-Jun/AP-1 (N) immunoreactivity in dying cells, as revealed with immunohistochemistry. Since the antibody to cJun/AP-1 (N) cross-reacts with epitopes emerging after caspase-mediated proteolysis during apoptosis, these results indicate that MAM-induced cell death is associated with active caspase-3 expression and function in dying cells. In order to investigate the involvement of tissue-type plasminogen activator (tPA), which has been implicated in certain forms of neuronal cell death, MAM-induced cell death has been examined in tPA-/- and tPA+/+ mice. No differences in the number of dying cells, as seen with haematoxylin and eosin staining and in situ end-labelling of fragmented nuclear DNA-processed sections, were seen between tPA-/- and tPA+/+ mice. These results indicate that tPA is not involved in MAM-induced cell death in the developing brain.
AB - Methylazoximethanol (MAM) acetate-induced cell death in the external granule cell layer of the developing cerebellum affects clusters of cells with morphological features of apoptosis. This is accompanied by selective induction of active caspase-3 expression and increased c-Jun/AP-1 (N) immunoreactivity in dying cells, as revealed with immunohistochemistry. Since the antibody to cJun/AP-1 (N) cross-reacts with epitopes emerging after caspase-mediated proteolysis during apoptosis, these results indicate that MAM-induced cell death is associated with active caspase-3 expression and function in dying cells. In order to investigate the involvement of tissue-type plasminogen activator (tPA), which has been implicated in certain forms of neuronal cell death, MAM-induced cell death has been examined in tPA-/- and tPA+/+ mice. No differences in the number of dying cells, as seen with haematoxylin and eosin staining and in situ end-labelling of fragmented nuclear DNA-processed sections, were seen between tPA-/- and tPA+/+ mice. These results indicate that tPA is not involved in MAM-induced cell death in the developing brain.
KW - Animals
KW - Animals, Newborn
KW - Antigens, CD95
KW - Apoptosis
KW - Caspase 3
KW - Caspases
KW - Cerebellar Cortex
KW - Fas Ligand Protein
KW - Membrane Glycoproteins
KW - Methylazoxymethanol Acetate
KW - Mice
KW - Mice, Knockout
KW - Neurons
KW - Nucleic Acid Synthesis Inhibitors
KW - Proto-Oncogene Proteins c-jun
KW - Signal Transduction
KW - Tissue Plasminogen Activator
M3 - SCORING: Journal article
C2 - 11166942
VL - 299
SP - 77
EP - 80
JO - NEUROSCI LETT
JF - NEUROSCI LETT
SN - 0304-3940
IS - 1-2
ER -