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Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator

Standard

Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator. / Ferrer, I; Puig, B; Goutan, E; Gombau, L; Muñoz-Cánoves, P; Puig Martorell, Berta.

in: NEUROSCI LETT, Jahrgang 299, Nr. 1-2, 16.02.2001, S. 77-80.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ferrer, I, Puig, B, Goutan, E, Gombau, L, Muñoz-Cánoves, P & Puig Martorell, B 2001, 'Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator', NEUROSCI LETT, Jg. 299, Nr. 1-2, S. 77-80.

APA

Ferrer, I., Puig, B., Goutan, E., Gombau, L., Muñoz-Cánoves, P., & Puig Martorell, B. (2001). Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator. NEUROSCI LETT, 299(1-2), 77-80.

Vancouver

Ferrer I, Puig B, Goutan E, Gombau L, Muñoz-Cánoves P, Puig Martorell B. Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator. NEUROSCI LETT. 2001 Feb 16;299(1-2):77-80.

Bibtex

@article{139b0f4f51374da798c6cd94b164cb14,
title = "Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator",
abstract = "Methylazoximethanol (MAM) acetate-induced cell death in the external granule cell layer of the developing cerebellum affects clusters of cells with morphological features of apoptosis. This is accompanied by selective induction of active caspase-3 expression and increased c-Jun/AP-1 (N) immunoreactivity in dying cells, as revealed with immunohistochemistry. Since the antibody to cJun/AP-1 (N) cross-reacts with epitopes emerging after caspase-mediated proteolysis during apoptosis, these results indicate that MAM-induced cell death is associated with active caspase-3 expression and function in dying cells. In order to investigate the involvement of tissue-type plasminogen activator (tPA), which has been implicated in certain forms of neuronal cell death, MAM-induced cell death has been examined in tPA-/- and tPA+/+ mice. No differences in the number of dying cells, as seen with haematoxylin and eosin staining and in situ end-labelling of fragmented nuclear DNA-processed sections, were seen between tPA-/- and tPA+/+ mice. These results indicate that tPA is not involved in MAM-induced cell death in the developing brain.",
keywords = "Animals, Animals, Newborn, Antigens, CD95, Apoptosis, Caspase 3, Caspases, Cerebellar Cortex, Fas Ligand Protein, Membrane Glycoproteins, Methylazoxymethanol Acetate, Mice, Mice, Knockout, Neurons, Nucleic Acid Synthesis Inhibitors, Proto-Oncogene Proteins c-jun, Signal Transduction, Tissue Plasminogen Activator",
author = "I Ferrer and B Puig and E Goutan and L Gombau and P Mu{\~n}oz-C{\'a}noves and {Puig Martorell}, Berta",
year = "2001",
month = feb,
day = "16",
language = "English",
volume = "299",
pages = "77--80",
journal = "NEUROSCI LETT",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

RIS

TY - JOUR

T1 - Methylazoximethanol acetate-induced cell death in the granule cell layer of the developing mouse cerebellum is associated with caspase-3 activation, but does not depend on the tissue-type plasminogen activator

AU - Ferrer, I

AU - Puig, B

AU - Goutan, E

AU - Gombau, L

AU - Muñoz-Cánoves, P

AU - Puig Martorell, Berta

PY - 2001/2/16

Y1 - 2001/2/16

N2 - Methylazoximethanol (MAM) acetate-induced cell death in the external granule cell layer of the developing cerebellum affects clusters of cells with morphological features of apoptosis. This is accompanied by selective induction of active caspase-3 expression and increased c-Jun/AP-1 (N) immunoreactivity in dying cells, as revealed with immunohistochemistry. Since the antibody to cJun/AP-1 (N) cross-reacts with epitopes emerging after caspase-mediated proteolysis during apoptosis, these results indicate that MAM-induced cell death is associated with active caspase-3 expression and function in dying cells. In order to investigate the involvement of tissue-type plasminogen activator (tPA), which has been implicated in certain forms of neuronal cell death, MAM-induced cell death has been examined in tPA-/- and tPA+/+ mice. No differences in the number of dying cells, as seen with haematoxylin and eosin staining and in situ end-labelling of fragmented nuclear DNA-processed sections, were seen between tPA-/- and tPA+/+ mice. These results indicate that tPA is not involved in MAM-induced cell death in the developing brain.

AB - Methylazoximethanol (MAM) acetate-induced cell death in the external granule cell layer of the developing cerebellum affects clusters of cells with morphological features of apoptosis. This is accompanied by selective induction of active caspase-3 expression and increased c-Jun/AP-1 (N) immunoreactivity in dying cells, as revealed with immunohistochemistry. Since the antibody to cJun/AP-1 (N) cross-reacts with epitopes emerging after caspase-mediated proteolysis during apoptosis, these results indicate that MAM-induced cell death is associated with active caspase-3 expression and function in dying cells. In order to investigate the involvement of tissue-type plasminogen activator (tPA), which has been implicated in certain forms of neuronal cell death, MAM-induced cell death has been examined in tPA-/- and tPA+/+ mice. No differences in the number of dying cells, as seen with haematoxylin and eosin staining and in situ end-labelling of fragmented nuclear DNA-processed sections, were seen between tPA-/- and tPA+/+ mice. These results indicate that tPA is not involved in MAM-induced cell death in the developing brain.

KW - Animals

KW - Animals, Newborn

KW - Antigens, CD95

KW - Apoptosis

KW - Caspase 3

KW - Caspases

KW - Cerebellar Cortex

KW - Fas Ligand Protein

KW - Membrane Glycoproteins

KW - Methylazoxymethanol Acetate

KW - Mice

KW - Mice, Knockout

KW - Neurons

KW - Nucleic Acid Synthesis Inhibitors

KW - Proto-Oncogene Proteins c-jun

KW - Signal Transduction

KW - Tissue Plasminogen Activator

M3 - SCORING: Journal article

C2 - 11166942

VL - 299

SP - 77

EP - 80

JO - NEUROSCI LETT

JF - NEUROSCI LETT

SN - 0304-3940

IS - 1-2

ER -