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Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription

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Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription. / Sercan Alp, Özen; Durlanik, Sibel; Schulz, Daniel; McGrath, Mairi; Grün, Joachim R; Bardua, Marcus; Ikuta, Koichi; Sgouroudis, Evridiki; Riedel, René; Zehentmeier, Sandra; Hauser, Anja E; Tsuneto, Motokazu; Melchers, Fritz; Tokoyoda, Koji; Chang, Hyun-Dong; Thiel, Andreas; Radbruch, Andreas.

In: EUR J IMMUNOL, Vol. 45, No. 4, 04.2015, p. 975-87.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Sercan Alp, Ö, Durlanik, S, Schulz, D, McGrath, M, Grün, JR, Bardua, M, Ikuta, K, Sgouroudis, E, Riedel, R, Zehentmeier, S, Hauser, AE, Tsuneto, M, Melchers, F, Tokoyoda, K, Chang, H-D, Thiel, A & Radbruch, A 2015, 'Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription', EUR J IMMUNOL, vol. 45, no. 4, pp. 975-87. https://doi.org/10.1002/eji.201445295

APA

Sercan Alp, Ö., Durlanik, S., Schulz, D., McGrath, M., Grün, J. R., Bardua, M., Ikuta, K., Sgouroudis, E., Riedel, R., Zehentmeier, S., Hauser, A. E., Tsuneto, M., Melchers, F., Tokoyoda, K., Chang, H-D., Thiel, A., & Radbruch, A. (2015). Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription. EUR J IMMUNOL, 45(4), 975-87. https://doi.org/10.1002/eji.201445295

Vancouver

Sercan Alp Ö, Durlanik S, Schulz D, McGrath M, Grün JR, Bardua M et al. Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription. EUR J IMMUNOL. 2015 Apr;45(4):975-87. https://doi.org/10.1002/eji.201445295

Bibtex

@article{841bd8012be84ab79b6aa11637e8dbc4,
title = "Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription",
abstract = "It is believed that memory CD8(+) T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) T cells individually colocalize with IL-7(+) reticular stromal cells. The T cells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-γ, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) T cells in BM are in G0 phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G2 of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory T cells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory T cells. Taken together, the present results suggest that CD8(+) memory T cells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling. ",
keywords = "Animals, Antigens, CD/biosynthesis, Antigens, Differentiation, T-Lymphocyte/biosynthesis, Bone Marrow Cells/cytology, CD8-Positive T-Lymphocytes/immunology, Cell Proliferation, Immunologic Memory/immunology, Interferon-gamma/biosynthesis, Interleukin-2/biosynthesis, Interleukin-7/immunology, Ki-67 Antigen/biosynthesis, Lectins, C-Type/biosynthesis, Mice, Mice, Inbred C57BL, Resting Phase, Cell Cycle/immunology, Stromal Cells/immunology, Transcription, Genetic, Tumor Necrosis Factor Receptor Superfamily, Member 9/biosynthesis",
author = "{Sercan Alp}, {\"O}zen and Sibel Durlanik and Daniel Schulz and Mairi McGrath and Gr{\"u}n, {Joachim R} and Marcus Bardua and Koichi Ikuta and Evridiki Sgouroudis and Ren{\'e} Riedel and Sandra Zehentmeier and Hauser, {Anja E} and Motokazu Tsuneto and Fritz Melchers and Koji Tokoyoda and Hyun-Dong Chang and Andreas Thiel and Andreas Radbruch",
note = "{\textcopyright} 2015 The Authors. European Journal of Immunology published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2015",
month = apr,
doi = "10.1002/eji.201445295",
language = "English",
volume = "45",
pages = "975--87",
journal = "EUR J IMMUNOL",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH",
number = "4",

}

RIS

TY - JOUR

T1 - Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription

AU - Sercan Alp, Özen

AU - Durlanik, Sibel

AU - Schulz, Daniel

AU - McGrath, Mairi

AU - Grün, Joachim R

AU - Bardua, Marcus

AU - Ikuta, Koichi

AU - Sgouroudis, Evridiki

AU - Riedel, René

AU - Zehentmeier, Sandra

AU - Hauser, Anja E

AU - Tsuneto, Motokazu

AU - Melchers, Fritz

AU - Tokoyoda, Koji

AU - Chang, Hyun-Dong

AU - Thiel, Andreas

AU - Radbruch, Andreas

N1 - © 2015 The Authors. European Journal of Immunology published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2015/4

Y1 - 2015/4

N2 - It is believed that memory CD8(+) T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) T cells individually colocalize with IL-7(+) reticular stromal cells. The T cells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-γ, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) T cells in BM are in G0 phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G2 of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory T cells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory T cells. Taken together, the present results suggest that CD8(+) memory T cells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.

AB - It is believed that memory CD8(+) T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) T cells individually colocalize with IL-7(+) reticular stromal cells. The T cells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-γ, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) T cells in BM are in G0 phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G2 of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory T cells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory T cells. Taken together, the present results suggest that CD8(+) memory T cells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.

KW - Animals

KW - Antigens, CD/biosynthesis

KW - Antigens, Differentiation, T-Lymphocyte/biosynthesis

KW - Bone Marrow Cells/cytology

KW - CD8-Positive T-Lymphocytes/immunology

KW - Cell Proliferation

KW - Immunologic Memory/immunology

KW - Interferon-gamma/biosynthesis

KW - Interleukin-2/biosynthesis

KW - Interleukin-7/immunology

KW - Ki-67 Antigen/biosynthesis

KW - Lectins, C-Type/biosynthesis

KW - Mice

KW - Mice, Inbred C57BL

KW - Resting Phase, Cell Cycle/immunology

KW - Stromal Cells/immunology

KW - Transcription, Genetic

KW - Tumor Necrosis Factor Receptor Superfamily, Member 9/biosynthesis

U2 - 10.1002/eji.201445295

DO - 10.1002/eji.201445295

M3 - SCORING: Journal article

C2 - 25639669

VL - 45

SP - 975

EP - 987

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 4

ER -