Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription
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Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription. / Sercan Alp, Özen; Durlanik, Sibel; Schulz, Daniel; McGrath, Mairi; Grün, Joachim R; Bardua, Marcus; Ikuta, Koichi; Sgouroudis, Evridiki; Riedel, René; Zehentmeier, Sandra; Hauser, Anja E; Tsuneto, Motokazu; Melchers, Fritz; Tokoyoda, Koji; Chang, Hyun-Dong; Thiel, Andreas; Radbruch, Andreas.
in: EUR J IMMUNOL, Jahrgang 45, Nr. 4, 04.2015, S. 975-87.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription
AU - Sercan Alp, Özen
AU - Durlanik, Sibel
AU - Schulz, Daniel
AU - McGrath, Mairi
AU - Grün, Joachim R
AU - Bardua, Marcus
AU - Ikuta, Koichi
AU - Sgouroudis, Evridiki
AU - Riedel, René
AU - Zehentmeier, Sandra
AU - Hauser, Anja E
AU - Tsuneto, Motokazu
AU - Melchers, Fritz
AU - Tokoyoda, Koji
AU - Chang, Hyun-Dong
AU - Thiel, Andreas
AU - Radbruch, Andreas
N1 - © 2015 The Authors. European Journal of Immunology published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/4
Y1 - 2015/4
N2 - It is believed that memory CD8(+) T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) T cells individually colocalize with IL-7(+) reticular stromal cells. The T cells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-γ, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) T cells in BM are in G0 phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G2 of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory T cells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory T cells. Taken together, the present results suggest that CD8(+) memory T cells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.
AB - It is believed that memory CD8(+) T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) T cells individually colocalize with IL-7(+) reticular stromal cells. The T cells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-γ, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) T cells in BM are in G0 phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G2 of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory T cells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory T cells. Taken together, the present results suggest that CD8(+) memory T cells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.
KW - Animals
KW - Antigens, CD/biosynthesis
KW - Antigens, Differentiation, T-Lymphocyte/biosynthesis
KW - Bone Marrow Cells/cytology
KW - CD8-Positive T-Lymphocytes/immunology
KW - Cell Proliferation
KW - Immunologic Memory/immunology
KW - Interferon-gamma/biosynthesis
KW - Interleukin-2/biosynthesis
KW - Interleukin-7/immunology
KW - Ki-67 Antigen/biosynthesis
KW - Lectins, C-Type/biosynthesis
KW - Mice
KW - Mice, Inbred C57BL
KW - Resting Phase, Cell Cycle/immunology
KW - Stromal Cells/immunology
KW - Transcription, Genetic
KW - Tumor Necrosis Factor Receptor Superfamily, Member 9/biosynthesis
U2 - 10.1002/eji.201445295
DO - 10.1002/eji.201445295
M3 - SCORING: Journal article
C2 - 25639669
VL - 45
SP - 975
EP - 987
JO - EUR J IMMUNOL
JF - EUR J IMMUNOL
SN - 0014-2980
IS - 4
ER -