Megahertz serial crystallography
Standard
Megahertz serial crystallography. / Wiedorn, Max O; Oberthür, Dominik; Bean, Richard; Schubert, Robin; Werner, Nadine; Abbey, Brian; Aepfelbacher, Martin; Adriano, Luigi; Allahgholi, Aschkan; Al-Qudami, Nasser; Andreasson, Jakob; Aplin, Steve; Awel, Salah; Ayyer, Kartik; Bajt, Saša; Barák, Imrich; Bari, Sadia; Bielecki, Johan; Botha, Sabine; Boukhelef, Djelloul; Brehm, Wolfgang; Brockhauser, Sandor; Cheviakov, Igor; Coleman, Matthew A; Cruz-Mazo, Francisco; Danilevski, Cyril; Darmanin, Connie; Doak, R Bruce; Domaracky, Martin; Dörner, Katerina; Du, Yang; Fangohr, Hans; Fleckenstein, Holger; Frank, Matthias; Fromme, Petra; Gañán-Calvo, Alfonso M; Gevorkov, Yaroslav; Giewekemeyer, Klaus; Ginn, Helen Mary; Graafsma, Heinz; Graceffa, Rita; Greiffenberg, Dominic; Gumprecht, Lars; Göttlicher, Peter; Hajdu, Janos; Hauf, Steffen; Heymann, Michael; Holmes, Susannah; Horke, Daniel A; Hunter, Mark S; Imlau, Siegfried; Kaukher, Alexander; Kim, Yoonhee; Klyuev, Alexander; Knoška, Juraj; Kobe, Bostjan; Kuhn, Manuela; Kupitz, Christopher; Küpper, Jochen; Lahey-Rudolph, Janine Mia; Laurus, Torsten; Le Cong, Karoline; Letrun, Romain; Xavier, P Lourdu; Maia, Luis; Maia, Filipe R N C; Mariani, Valerio; Messerschmidt, Marc; Metz, Markus; Mezza, Davide; Michelat, Thomas; Mills, Grant; Monteiro, Diana C F; Morgan, Andrew; Mühlig, Kerstin; Munke, Anna; Münnich, Astrid; Nette, Julia; Nugent, Keith A; Nuguid, Theresa; Orville, Allen M; Pandey, Suraj; Pena, Gisel; Villanueva-Perez, Pablo; Poehlsen, Jennifer; Previtali, Gianpietro; Redecke, Lars; Riekehr, Winnie Maria; Rohde, Holger; Round, Adam; Safenreiter, Tatiana; Sarrou, Iosifina; Sato, Tokushi; Schmidt, Marius; Schmitt, Bernd; Schönherr, Robert; Schulz, Joachim; Sellberg, Jonas A; Seibert, M Marvin; Seuring, Carolin; Shelby, Megan L; Shoeman, Robert L; Sikorski, Marcin; Silenzi, Alessandro; Stan, Claudiu A; Shi, Xintian; Stern, Stephan; Sztuk-Dambietz, Jola; Szuba, Janusz; Tolstikova, Aleksandra; Trebbin, Martin; Trunk, Ulrich; Vagovic, Patrik; Ve, Thomas; Weinhausen, Britta; White, Thomas A; Wrona, Krzysztof; Xu, Chen; Yefanov, Oleksandr; Zatsepin, Nadia; Zhang, Jiaguo; Perbandt, Markus; Mancuso, Adrian P; Betzel, Christian; Chapman, Henry; Barty, Anton.
In: NAT COMMUN, Vol. 9, No. 1, 02.10.2018, p. 4025.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Megahertz serial crystallography
AU - Wiedorn, Max O
AU - Oberthür, Dominik
AU - Bean, Richard
AU - Schubert, Robin
AU - Werner, Nadine
AU - Abbey, Brian
AU - Aepfelbacher, Martin
AU - Adriano, Luigi
AU - Allahgholi, Aschkan
AU - Al-Qudami, Nasser
AU - Andreasson, Jakob
AU - Aplin, Steve
AU - Awel, Salah
AU - Ayyer, Kartik
AU - Bajt, Saša
AU - Barák, Imrich
AU - Bari, Sadia
AU - Bielecki, Johan
AU - Botha, Sabine
AU - Boukhelef, Djelloul
AU - Brehm, Wolfgang
AU - Brockhauser, Sandor
AU - Cheviakov, Igor
AU - Coleman, Matthew A
AU - Cruz-Mazo, Francisco
AU - Danilevski, Cyril
AU - Darmanin, Connie
AU - Doak, R Bruce
AU - Domaracky, Martin
AU - Dörner, Katerina
AU - Du, Yang
AU - Fangohr, Hans
AU - Fleckenstein, Holger
AU - Frank, Matthias
AU - Fromme, Petra
AU - Gañán-Calvo, Alfonso M
AU - Gevorkov, Yaroslav
AU - Giewekemeyer, Klaus
AU - Ginn, Helen Mary
AU - Graafsma, Heinz
AU - Graceffa, Rita
AU - Greiffenberg, Dominic
AU - Gumprecht, Lars
AU - Göttlicher, Peter
AU - Hajdu, Janos
AU - Hauf, Steffen
AU - Heymann, Michael
AU - Holmes, Susannah
AU - Horke, Daniel A
AU - Hunter, Mark S
AU - Imlau, Siegfried
AU - Kaukher, Alexander
AU - Kim, Yoonhee
AU - Klyuev, Alexander
AU - Knoška, Juraj
AU - Kobe, Bostjan
AU - Kuhn, Manuela
AU - Kupitz, Christopher
AU - Küpper, Jochen
AU - Lahey-Rudolph, Janine Mia
AU - Laurus, Torsten
AU - Le Cong, Karoline
AU - Letrun, Romain
AU - Xavier, P Lourdu
AU - Maia, Luis
AU - Maia, Filipe R N C
AU - Mariani, Valerio
AU - Messerschmidt, Marc
AU - Metz, Markus
AU - Mezza, Davide
AU - Michelat, Thomas
AU - Mills, Grant
AU - Monteiro, Diana C F
AU - Morgan, Andrew
AU - Mühlig, Kerstin
AU - Munke, Anna
AU - Münnich, Astrid
AU - Nette, Julia
AU - Nugent, Keith A
AU - Nuguid, Theresa
AU - Orville, Allen M
AU - Pandey, Suraj
AU - Pena, Gisel
AU - Villanueva-Perez, Pablo
AU - Poehlsen, Jennifer
AU - Previtali, Gianpietro
AU - Redecke, Lars
AU - Riekehr, Winnie Maria
AU - Rohde, Holger
AU - Round, Adam
AU - Safenreiter, Tatiana
AU - Sarrou, Iosifina
AU - Sato, Tokushi
AU - Schmidt, Marius
AU - Schmitt, Bernd
AU - Schönherr, Robert
AU - Schulz, Joachim
AU - Sellberg, Jonas A
AU - Seibert, M Marvin
AU - Seuring, Carolin
AU - Shelby, Megan L
AU - Shoeman, Robert L
AU - Sikorski, Marcin
AU - Silenzi, Alessandro
AU - Stan, Claudiu A
AU - Shi, Xintian
AU - Stern, Stephan
AU - Sztuk-Dambietz, Jola
AU - Szuba, Janusz
AU - Tolstikova, Aleksandra
AU - Trebbin, Martin
AU - Trunk, Ulrich
AU - Vagovic, Patrik
AU - Ve, Thomas
AU - Weinhausen, Britta
AU - White, Thomas A
AU - Wrona, Krzysztof
AU - Xu, Chen
AU - Yefanov, Oleksandr
AU - Zatsepin, Nadia
AU - Zhang, Jiaguo
AU - Perbandt, Markus
AU - Mancuso, Adrian P
AU - Betzel, Christian
AU - Chapman, Henry
AU - Barty, Anton
PY - 2018/10/2
Y1 - 2018/10/2
N2 - The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
AB - The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
KW - Journal Article
U2 - 10.1038/s41467-018-06156-7
DO - 10.1038/s41467-018-06156-7
M3 - SCORING: Journal article
C2 - 30279492
VL - 9
SP - 4025
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
IS - 1
ER -