Megahertz serial crystallography

Max O Wiedorn; Dominik Oberthür; Richard Bean; Robin Schubert; Nadine Werner; Brian Abbey; Martin Aepfelbacher; Luigi Adriano; Aschkan Allahgholi; Nasser Al-Qudami; Jakob Andreasson; Steve Aplin; Salah Awel; Kartik Ayyer; Saša Bajt; Imrich Barák; Sadia Bari; Johan Bielecki; Sabine Botha; Djelloul Boukhelef; Wolfgang Brehm; Sandor Brockhauser; Igor Cheviakov; Matthew A Coleman; Francisco Cruz-Mazo; Cyril Danilevski; Connie Darmanin; R Bruce Doak; Martin Domaracky; Katerina Dörner; Yang Du; Hans Fangohr; Holger Fleckenstein; Matthias Frank; Petra Fromme; Alfonso M Gañán-Calvo; Yaroslav Gevorkov; Klaus Giewekemeyer; Helen Mary Ginn; Heinz Graafsma; Rita Graceffa; Dominic Greiffenberg; Lars Gumprecht; Peter Göttlicher; Janos Hajdu; Steffen Hauf; Michael Heymann; Susannah Holmes; Daniel A Horke; Mark S Hunter; Siegfried Imlau; Alexander Kaukher; Yoonhee Kim; Alexander Klyuev; Juraj Knoška; Bostjan Kobe; Manuela Kuhn; Christopher Kupitz; Jochen Küpper; Janine Mia Lahey-Rudolph; Torsten Laurus; Karoline Le Cong; Romain Letrun; P Lourdu Xavier; Luis Maia; Filipe R N C Maia; Valerio Mariani; Marc Messerschmidt; Markus Metz; Davide Mezza; Thomas Michelat; Grant Mills; Diana C F Monteiro; Andrew Morgan; Kerstin Mühlig; Anna Munke; Astrid Münnich; Julia Nette; Keith A Nugent; Theresa Nuguid; Allen M Orville; Suraj Pandey; Gisel Pena; Pablo Villanueva-Perez; Jennifer Poehlsen; Gianpietro Previtali; Lars Redecke; Winnie Maria Riekehr; Holger Rohde; Adam Round; Tatiana Safenreiter; Iosifina Sarrou; Tokushi Sato; Marius Schmidt; Bernd Schmitt; Robert Schönherr; Joachim Schulz; Jonas A Sellberg; M Marvin Seibert; Carolin Seuring; Megan L Shelby; Robert L Shoeman; Marcin Sikorski; Alessandro Silenzi; Claudiu A Stan; Xintian Shi; Stephan Stern; Jola Sztuk-Dambietz; Janusz Szuba; Aleksandra Tolstikova; Martin Trebbin; Ulrich Trunk; Patrik Vagovic; Thomas Ve; Britta Weinhausen; Thomas A White; Krzysztof Wrona; Chen Xu; Oleksandr Yefanov; Nadia Zatsepin; Jiaguo Zhang; Markus Perbandt; Adrian P Mancuso; Christian Betzel; Henry Chapman; Anton Barty

doi:10.1038/s41467-018-06156-7

Megahertz serial crystallography

Standard

Megahertz serial crystallography. / Wiedorn, Max O; Oberthür, Dominik; Bean, Richard; Schubert, Robin; Werner, Nadine; Abbey, Brian; Aepfelbacher, Martin; Adriano, Luigi; Allahgholi, Aschkan; Al-Qudami, Nasser; Andreasson, Jakob; Aplin, Steve; Awel, Salah; Ayyer, Kartik; Bajt, Saša; Barák, Imrich; Bari, Sadia; Bielecki, Johan; Botha, Sabine; Boukhelef, Djelloul; Brehm, Wolfgang; Brockhauser, Sandor; Cheviakov, Igor; Coleman, Matthew A; Cruz-Mazo, Francisco; Danilevski, Cyril; Darmanin, Connie; Doak, R Bruce; Domaracky, Martin; Dörner, Katerina; Du, Yang; Fangohr, Hans; Fleckenstein, Holger; Frank, Matthias; Fromme, Petra; Gañán-Calvo, Alfonso M; Gevorkov, Yaroslav; Giewekemeyer, Klaus; Ginn, Helen Mary; Graafsma, Heinz; Graceffa, Rita; Greiffenberg, Dominic; Gumprecht, Lars; Göttlicher, Peter; Hajdu, Janos; Hauf, Steffen; Heymann, Michael; Holmes, Susannah; Horke, Daniel A; Hunter, Mark S; Imlau, Siegfried; Kaukher, Alexander; Kim, Yoonhee; Klyuev, Alexander; Knoška, Juraj; Kobe, Bostjan; Kuhn, Manuela; Kupitz, Christopher; Küpper, Jochen; Lahey-Rudolph, Janine Mia; Laurus, Torsten; Le Cong, Karoline; Letrun, Romain; Xavier, P Lourdu; Maia, Luis; Maia, Filipe R N C; Mariani, Valerio; Messerschmidt, Marc; Metz, Markus; Mezza, Davide; Michelat, Thomas; Mills, Grant; Monteiro, Diana C F; Morgan, Andrew; Mühlig, Kerstin; Munke, Anna; Münnich, Astrid; Nette, Julia; Nugent, Keith A; Nuguid, Theresa; Orville, Allen M; Pandey, Suraj; Pena, Gisel; Villanueva-Perez, Pablo; Poehlsen, Jennifer; Previtali, Gianpietro; Redecke, Lars; Riekehr, Winnie Maria; Rohde, Holger; Round, Adam; Safenreiter, Tatiana; Sarrou, Iosifina; Sato, Tokushi; Schmidt, Marius; Schmitt, Bernd; Schönherr, Robert; Schulz, Joachim; Sellberg, Jonas A; Seibert, M Marvin; Seuring, Carolin; Shelby, Megan L; Shoeman, Robert L; Sikorski, Marcin; Silenzi, Alessandro; Stan, Claudiu A; Shi, Xintian; Stern, Stephan; Sztuk-Dambietz, Jola; Szuba, Janusz; Tolstikova, Aleksandra; Trebbin, Martin; Trunk, Ulrich; Vagovic, Patrik; Ve, Thomas; Weinhausen, Britta; White, Thomas A; Wrona, Krzysztof; Xu, Chen; Yefanov, Oleksandr; Zatsepin, Nadia; Zhang, Jiaguo; Perbandt, Markus; Mancuso, Adrian P; Betzel, Christian; Chapman, Henry; Barty, Anton.

in: NAT COMMUN, Jahrgang 9, Nr. 1, 02.10.2018, S. 4025.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Wiedorn, MO, Oberthür, D, Bean, R, Schubert, R, Werner, N, Abbey, B, Aepfelbacher, M, Adriano, L, Allahgholi, A, Al-Qudami, N, Andreasson, J, Aplin, S, Awel, S, Ayyer, K, Bajt, S, Barák, I, Bari, S, Bielecki, J, Botha, S, Boukhelef, D, Brehm, W, Brockhauser, S, Cheviakov, I, Coleman, MA, Cruz-Mazo, F, Danilevski, C, Darmanin, C, Doak, RB, Domaracky, M, Dörner, K, Du, Y, Fangohr, H, Fleckenstein, H, Frank, M, Fromme, P, Gañán-Calvo, AM, Gevorkov, Y, Giewekemeyer, K, Ginn, HM, Graafsma, H, Graceffa, R, Greiffenberg, D, Gumprecht, L, Göttlicher, P, Hajdu, J, Hauf, S, Heymann, M, Holmes, S, Horke, DA, Hunter, MS, Imlau, S, Kaukher, A, Kim, Y, Klyuev, A, Knoška, J, Kobe, B, Kuhn, M, Kupitz, C, Küpper, J, Lahey-Rudolph, JM, Laurus, T, Le Cong, K, Letrun, R, Xavier, PL, Maia, L, Maia, FRNC, Mariani, V, Messerschmidt, M, Metz, M, Mezza, D, Michelat, T, Mills, G, Monteiro, DCF, Morgan, A, Mühlig, K, Munke, A, Münnich, A, Nette, J, Nugent, KA, Nuguid, T, Orville, AM, Pandey, S, Pena, G, Villanueva-Perez, P, Poehlsen, J, Previtali, G, Redecke, L, Riekehr, WM, Rohde, H, Round, A, Safenreiter, T, Sarrou, I, Sato, T, Schmidt, M, Schmitt, B, Schönherr, R, Schulz, J, Sellberg, JA, Seibert, MM, Seuring, C, Shelby, ML, Shoeman, RL, Sikorski, M, Silenzi, A, Stan, CA, Shi, X, Stern, S, Sztuk-Dambietz, J, Szuba, J, Tolstikova, A, Trebbin, M, Trunk, U, Vagovic, P, Ve, T, Weinhausen, B, White, TA, Wrona, K, Xu, C, Yefanov, O, Zatsepin, N, Zhang, J, Perbandt, M, Mancuso, AP, Betzel, C, Chapman, H & Barty, A 2018, 'Megahertz serial crystallography', NAT COMMUN, Jg. 9, Nr. 1, S. 4025. https://doi.org/10.1038/s41467-018-06156-7

APA

Wiedorn, M. O., Oberthür, D., Bean, R., Schubert, R., Werner, N., Abbey, B., Aepfelbacher, M., Adriano, L., Allahgholi, A., Al-Qudami, N., Andreasson, J., Aplin, S., Awel, S., Ayyer, K., Bajt, S., Barák, I., Bari, S., Bielecki, J., Botha, S., ... Barty, A. (2018). Megahertz serial crystallography. NAT COMMUN, 9(1), 4025. https://doi.org/10.1038/s41467-018-06156-7

Vancouver

Wiedorn MO, Oberthür D, Bean R, Schubert R, Werner N, Abbey B et al. Megahertz serial crystallography. NAT COMMUN. 2018 Okt 2;9(1):4025. https://doi.org/10.1038/s41467-018-06156-7

Bibtex

@article{c3e03040987d4799848844fa239bf774,

title = "Megahertz serial crystallography",

abstract = "The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.",

keywords = "Journal Article",

author = "Wiedorn, {Max O} and Dominik Oberth{\"u}r and Richard Bean and Robin Schubert and Nadine Werner and Brian Abbey and Martin Aepfelbacher and Luigi Adriano and Aschkan Allahgholi and Nasser Al-Qudami and Jakob Andreasson and Steve Aplin and Salah Awel and Kartik Ayyer and Sa{\v s}a Bajt and Imrich Bar{\'a}k and Sadia Bari and Johan Bielecki and Sabine Botha and Djelloul Boukhelef and Wolfgang Brehm and Sandor Brockhauser and Igor Cheviakov and Coleman, {Matthew A} and Francisco Cruz-Mazo and Cyril Danilevski and Connie Darmanin and Doak, {R Bruce} and Martin Domaracky and Katerina D{\"o}rner and Yang Du and Hans Fangohr and Holger Fleckenstein and Matthias Frank and Petra Fromme and Ga{\~n}{\'a}n-Calvo, {Alfonso M} and Yaroslav Gevorkov and Klaus Giewekemeyer and Ginn, {Helen Mary} and Heinz Graafsma and Rita Graceffa and Dominic Greiffenberg and Lars Gumprecht and Peter G{\"o}ttlicher and Janos Hajdu and Steffen Hauf and Michael Heymann and Susannah Holmes and Horke, {Daniel A} and Hunter, {Mark S} and Siegfried Imlau and Alexander Kaukher and Yoonhee Kim and Alexander Klyuev and Juraj Kno{\v s}ka and Bostjan Kobe and Manuela Kuhn and Christopher Kupitz and Jochen K{\"u}pper and Lahey-Rudolph, {Janine Mia} and Torsten Laurus and {Le Cong}, Karoline and Romain Letrun and Xavier, {P Lourdu} and Luis Maia and Maia, {Filipe R N C} and Valerio Mariani and Marc Messerschmidt and Markus Metz and Davide Mezza and Thomas Michelat and Grant Mills and Monteiro, {Diana C F} and Andrew Morgan and Kerstin M{\"u}hlig and Anna Munke and Astrid M{\"u}nnich and Julia Nette and Nugent, {Keith A} and Theresa Nuguid and Orville, {Allen M} and Suraj Pandey and Gisel Pena and Pablo Villanueva-Perez and Jennifer Poehlsen and Gianpietro Previtali and Lars Redecke and Riekehr, {Winnie Maria} and Holger Rohde and Adam Round and Tatiana Safenreiter and Iosifina Sarrou and Tokushi Sato and Marius Schmidt and Bernd Schmitt and Robert Sch{\"o}nherr and Joachim Schulz and Sellberg, {Jonas A} and Seibert, {M Marvin} and Carolin Seuring and Shelby, {Megan L} and Shoeman, {Robert L} and Marcin Sikorski and Alessandro Silenzi and Stan, {Claudiu A} and Xintian Shi and Stephan Stern and Jola Sztuk-Dambietz and Janusz Szuba and Aleksandra Tolstikova and Martin Trebbin and Ulrich Trunk and Patrik Vagovic and Thomas Ve and Britta Weinhausen and White, {Thomas A} and Krzysztof Wrona and Chen Xu and Oleksandr Yefanov and Nadia Zatsepin and Jiaguo Zhang and Markus Perbandt and Mancuso, {Adrian P} and Christian Betzel and Henry Chapman and Anton Barty",

year = "2018",

month = oct,

day = "2",

doi = "10.1038/s41467-018-06156-7",

language = "English",

volume = "9",

pages = "4025",

journal = "NAT COMMUN",

issn = "2041-1723",

publisher = "NATURE PUBLISHING GROUP",

number = "1",

}

RIS

TY - JOUR

T1 - Megahertz serial crystallography

AU - Wiedorn, Max O

AU - Oberthür, Dominik

AU - Bean, Richard

AU - Schubert, Robin

AU - Werner, Nadine

AU - Abbey, Brian

AU - Aepfelbacher, Martin

AU - Adriano, Luigi

AU - Allahgholi, Aschkan

AU - Al-Qudami, Nasser

AU - Andreasson, Jakob

AU - Aplin, Steve

AU - Awel, Salah

AU - Ayyer, Kartik

AU - Bajt, Saša

AU - Barák, Imrich

AU - Bari, Sadia

AU - Bielecki, Johan

AU - Botha, Sabine

AU - Boukhelef, Djelloul

AU - Brehm, Wolfgang

AU - Brockhauser, Sandor

AU - Cheviakov, Igor

AU - Coleman, Matthew A

AU - Cruz-Mazo, Francisco

AU - Danilevski, Cyril

AU - Darmanin, Connie

AU - Doak, R Bruce

AU - Domaracky, Martin

AU - Dörner, Katerina

AU - Du, Yang

AU - Fangohr, Hans

AU - Fleckenstein, Holger

AU - Frank, Matthias

AU - Fromme, Petra

AU - Gañán-Calvo, Alfonso M

AU - Gevorkov, Yaroslav

AU - Giewekemeyer, Klaus

AU - Ginn, Helen Mary

AU - Graafsma, Heinz

AU - Graceffa, Rita

AU - Greiffenberg, Dominic

AU - Gumprecht, Lars

AU - Göttlicher, Peter

AU - Hajdu, Janos

AU - Hauf, Steffen

AU - Heymann, Michael

AU - Holmes, Susannah

AU - Horke, Daniel A

AU - Hunter, Mark S

AU - Imlau, Siegfried

AU - Kaukher, Alexander

AU - Kim, Yoonhee

AU - Klyuev, Alexander

AU - Knoška, Juraj

AU - Kobe, Bostjan

AU - Kuhn, Manuela

AU - Kupitz, Christopher

AU - Küpper, Jochen

AU - Lahey-Rudolph, Janine Mia

AU - Laurus, Torsten

AU - Le Cong, Karoline

AU - Letrun, Romain

AU - Xavier, P Lourdu

AU - Maia, Luis

AU - Maia, Filipe R N C

AU - Mariani, Valerio

AU - Messerschmidt, Marc

AU - Metz, Markus

AU - Mezza, Davide

AU - Michelat, Thomas

AU - Mills, Grant

AU - Monteiro, Diana C F

AU - Morgan, Andrew

AU - Mühlig, Kerstin

AU - Munke, Anna

AU - Münnich, Astrid

AU - Nette, Julia

AU - Nugent, Keith A

AU - Nuguid, Theresa

AU - Orville, Allen M

AU - Pandey, Suraj

AU - Pena, Gisel

AU - Villanueva-Perez, Pablo

AU - Poehlsen, Jennifer

AU - Previtali, Gianpietro

AU - Redecke, Lars

AU - Riekehr, Winnie Maria

AU - Rohde, Holger

AU - Round, Adam

AU - Safenreiter, Tatiana

AU - Sarrou, Iosifina

AU - Sato, Tokushi

AU - Schmidt, Marius

AU - Schmitt, Bernd

AU - Schönherr, Robert

AU - Schulz, Joachim

AU - Sellberg, Jonas A

AU - Seibert, M Marvin

AU - Seuring, Carolin

AU - Shelby, Megan L

AU - Shoeman, Robert L

AU - Sikorski, Marcin

AU - Silenzi, Alessandro

AU - Stan, Claudiu A

AU - Shi, Xintian

AU - Stern, Stephan

AU - Sztuk-Dambietz, Jola

AU - Szuba, Janusz

AU - Tolstikova, Aleksandra

AU - Trebbin, Martin

AU - Trunk, Ulrich

AU - Vagovic, Patrik

AU - Ve, Thomas

AU - Weinhausen, Britta

AU - White, Thomas A

AU - Wrona, Krzysztof

AU - Xu, Chen

AU - Yefanov, Oleksandr

AU - Zatsepin, Nadia

AU - Zhang, Jiaguo

AU - Perbandt, Markus

AU - Mancuso, Adrian P

AU - Betzel, Christian

AU - Chapman, Henry

AU - Barty, Anton

PY - 2018/10/2

Y1 - 2018/10/2

N2 - The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

AB - The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

KW - Journal Article

U2 - 10.1038/s41467-018-06156-7

DO - 10.1038/s41467-018-06156-7

M3 - SCORING: Journal article

C2 - 30279492

VL - 9

SP - 4025

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -