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Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking

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Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. / Cervero, Pasquale; Wiesner, Christiane; Bouissou, Anais; Poincloux, Renaud; Linder, Stefan.

In: NAT COMMUN, Vol. 9, No. 1, 06.02.2018, p. 515.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Cervero, P, Wiesner, C, Bouissou, A, Poincloux, R & Linder, S 2018, 'Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking', NAT COMMUN, vol. 9, no. 1, pp. 515. https://doi.org/10.1038/s41467-018-02904-x

APA

Cervero, P., Wiesner, C., Bouissou, A., Poincloux, R., & Linder, S. (2018). Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. NAT COMMUN, 9(1), 515. https://doi.org/10.1038/s41467-018-02904-x

Vancouver

Cervero P, Wiesner C, Bouissou A, Poincloux R, Linder S. Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking. NAT COMMUN. 2018 Feb 6;9(1):515. https://doi.org/10.1038/s41467-018-02904-x

Bibtex

@article{3c4fb247200945ce87d035555b795884,
title = "Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking",
abstract = "Subcellular fine-tuning of the actomyosin cytoskeleton is a prerequisite for polarized cell migration. We identify LSP (lymphocyte-specific protein) 1 as a critical regulator of actomyosin contractility in primary macrophages. LSP1 regulates adhesion and migration, including the parameters cell area and speed, and also podosome turnover, oscillation and protrusive force. LSP1 recruits myosin IIA and its regulators, including myosin light chain kinase and calmodulin, and competes with supervillin, a myosin hyperactivator, for myosin regulators, and for actin isoforms, notably β-actin. Actin isoforms are anisotropically distributed in myosin IIA-expressing macrophages, and contribute to the differential recruitment of LSP1 and supervillin, thus enabling an actomyosin symmetry break, analogous to the situation in cells expressing two myosin II isoforms. Collectively, these results show that the cellular pattern of actin isoforms builds the basis for the differential distribution of two actomyosin machineries with distinct properties, leading to the establishment of discrete zones of actomyosin contractility.",
keywords = "Journal Article",
author = "Pasquale Cervero and Christiane Wiesner and Anais Bouissou and Renaud Poincloux and Stefan Linder",
year = "2018",
month = feb,
day = "6",
doi = "10.1038/s41467-018-02904-x",
language = "English",
volume = "9",
pages = "515",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking

AU - Cervero, Pasquale

AU - Wiesner, Christiane

AU - Bouissou, Anais

AU - Poincloux, Renaud

AU - Linder, Stefan

PY - 2018/2/6

Y1 - 2018/2/6

N2 - Subcellular fine-tuning of the actomyosin cytoskeleton is a prerequisite for polarized cell migration. We identify LSP (lymphocyte-specific protein) 1 as a critical regulator of actomyosin contractility in primary macrophages. LSP1 regulates adhesion and migration, including the parameters cell area and speed, and also podosome turnover, oscillation and protrusive force. LSP1 recruits myosin IIA and its regulators, including myosin light chain kinase and calmodulin, and competes with supervillin, a myosin hyperactivator, for myosin regulators, and for actin isoforms, notably β-actin. Actin isoforms are anisotropically distributed in myosin IIA-expressing macrophages, and contribute to the differential recruitment of LSP1 and supervillin, thus enabling an actomyosin symmetry break, analogous to the situation in cells expressing two myosin II isoforms. Collectively, these results show that the cellular pattern of actin isoforms builds the basis for the differential distribution of two actomyosin machineries with distinct properties, leading to the establishment of discrete zones of actomyosin contractility.

AB - Subcellular fine-tuning of the actomyosin cytoskeleton is a prerequisite for polarized cell migration. We identify LSP (lymphocyte-specific protein) 1 as a critical regulator of actomyosin contractility in primary macrophages. LSP1 regulates adhesion and migration, including the parameters cell area and speed, and also podosome turnover, oscillation and protrusive force. LSP1 recruits myosin IIA and its regulators, including myosin light chain kinase and calmodulin, and competes with supervillin, a myosin hyperactivator, for myosin regulators, and for actin isoforms, notably β-actin. Actin isoforms are anisotropically distributed in myosin IIA-expressing macrophages, and contribute to the differential recruitment of LSP1 and supervillin, thus enabling an actomyosin symmetry break, analogous to the situation in cells expressing two myosin II isoforms. Collectively, these results show that the cellular pattern of actin isoforms builds the basis for the differential distribution of two actomyosin machineries with distinct properties, leading to the establishment of discrete zones of actomyosin contractility.

KW - Journal Article

U2 - 10.1038/s41467-018-02904-x

DO - 10.1038/s41467-018-02904-x

M3 - SCORING: Journal article

C2 - 29410425

VL - 9

SP - 515

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -