Luminography--a new, highly sensitive visualization method for electrophoresis
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Luminography--a new, highly sensitive visualization method for electrophoresis. / Schneppenheim, R; Budde, U; Dahlmann, N; Rautenberg, P.
In: ELECTROPHORESIS, Vol. 12, No. 5, 01.05.1991, p. 367-72.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Luminography--a new, highly sensitive visualization method for electrophoresis
AU - Schneppenheim, R
AU - Budde, U
AU - Dahlmann, N
AU - Rautenberg, P
PY - 1991/5/1
Y1 - 1991/5/1
N2 - A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.
AB - A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.
KW - Animals
KW - Antibodies, Heterophile
KW - Autoradiography
KW - Blotting, Western
KW - Chromogenic Compounds
KW - Densitometry
KW - Electrophoresis, Polyacrylamide Gel
KW - Gliadin
KW - HIV Antibodies
KW - Horseradish Peroxidase
KW - Humans
KW - Hydrogen Peroxide
KW - Immunoglobulin G
KW - Isoelectric Focusing
KW - Luminescent Measurements
KW - Luminol
KW - Mice
KW - Transglutaminases
KW - von Willebrand Factor
U2 - 10.1002/elps.1150120508
DO - 10.1002/elps.1150120508
M3 - SCORING: Journal article
C2 - 1682145
VL - 12
SP - 367
EP - 372
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
SN - 0173-0835
IS - 5
ER -