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Luminography--a new, highly sensitive visualization method for electrophoresis

Standard

Luminography--a new, highly sensitive visualization method for electrophoresis. / Schneppenheim, R; Budde, U; Dahlmann, N; Rautenberg, P.

in: ELECTROPHORESIS, Jahrgang 12, Nr. 5, 01.05.1991, S. 367-72.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schneppenheim, R, Budde, U, Dahlmann, N & Rautenberg, P 1991, 'Luminography--a new, highly sensitive visualization method for electrophoresis', ELECTROPHORESIS, Jg. 12, Nr. 5, S. 367-72. https://doi.org/10.1002/elps.1150120508

APA

Schneppenheim, R., Budde, U., Dahlmann, N., & Rautenberg, P. (1991). Luminography--a new, highly sensitive visualization method for electrophoresis. ELECTROPHORESIS, 12(5), 367-72. https://doi.org/10.1002/elps.1150120508

Vancouver

Schneppenheim R, Budde U, Dahlmann N, Rautenberg P. Luminography--a new, highly sensitive visualization method for electrophoresis. ELECTROPHORESIS. 1991 Mai 1;12(5):367-72. https://doi.org/10.1002/elps.1150120508

Bibtex

@article{8805c1f207834aa48a52dd95520c4997,
title = "Luminography--a new, highly sensitive visualization method for electrophoresis",
abstract = "A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.",
keywords = "Animals, Antibodies, Heterophile, Autoradiography, Blotting, Western, Chromogenic Compounds, Densitometry, Electrophoresis, Polyacrylamide Gel, Gliadin, HIV Antibodies, Horseradish Peroxidase, Humans, Hydrogen Peroxide, Immunoglobulin G, Isoelectric Focusing, Luminescent Measurements, Luminol, Mice, Transglutaminases, von Willebrand Factor",
author = "R Schneppenheim and U Budde and N Dahlmann and P Rautenberg",
year = "1991",
month = may,
day = "1",
doi = "10.1002/elps.1150120508",
language = "English",
volume = "12",
pages = "367--72",
journal = "ELECTROPHORESIS",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag GmbH",
number = "5",

}

RIS

TY - JOUR

T1 - Luminography--a new, highly sensitive visualization method for electrophoresis

AU - Schneppenheim, R

AU - Budde, U

AU - Dahlmann, N

AU - Rautenberg, P

PY - 1991/5/1

Y1 - 1991/5/1

N2 - A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.

AB - A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.

KW - Animals

KW - Antibodies, Heterophile

KW - Autoradiography

KW - Blotting, Western

KW - Chromogenic Compounds

KW - Densitometry

KW - Electrophoresis, Polyacrylamide Gel

KW - Gliadin

KW - HIV Antibodies

KW - Horseradish Peroxidase

KW - Humans

KW - Hydrogen Peroxide

KW - Immunoglobulin G

KW - Isoelectric Focusing

KW - Luminescent Measurements

KW - Luminol

KW - Mice

KW - Transglutaminases

KW - von Willebrand Factor

U2 - 10.1002/elps.1150120508

DO - 10.1002/elps.1150120508

M3 - SCORING: Journal article

C2 - 1682145

VL - 12

SP - 367

EP - 372

JO - ELECTROPHORESIS

JF - ELECTROPHORESIS

SN - 0173-0835

IS - 5

ER -