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Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes

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Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes. / Budde, U; Schneppenheim, R; Plendl, H; Dent, J; Ruggeri, Z M; Zimmerman, T S.

In: THROMB HAEMOSTASIS, Vol. 63, No. 2, 12.04.1990, p. 312-5.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Budde, U, Schneppenheim, R, Plendl, H, Dent, J, Ruggeri, ZM & Zimmerman, TS 1990, 'Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes', THROMB HAEMOSTASIS, vol. 63, no. 2, pp. 312-5.

APA

Budde, U., Schneppenheim, R., Plendl, H., Dent, J., Ruggeri, Z. M., & Zimmerman, T. S. (1990). Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes. THROMB HAEMOSTASIS, 63(2), 312-5.

Vancouver

Budde U, Schneppenheim R, Plendl H, Dent J, Ruggeri ZM, Zimmerman TS. Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes. THROMB HAEMOSTASIS. 1990 Apr 12;63(2):312-5.

Bibtex

@article{aa64b1fee95647f0a22998e2d9ef467b,
title = "Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes",
abstract = "Two methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, IIE, and IIF. Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf:Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf:Ag detectable after 1:300 dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.",
keywords = "Autoradiography, Blotting, Western, Collodion, Electrophoresis, Agar Gel, Humans, Luminescent Measurements, Macromolecular Substances, Membranes, Artificial, von Willebrand Factor",
author = "U Budde and R Schneppenheim and H Plendl and J Dent and Ruggeri, {Z M} and Zimmerman, {T S}",
year = "1990",
month = apr,
day = "12",
language = "English",
volume = "63",
pages = "312--5",
journal = "THROMB HAEMOSTASIS",
issn = "0340-6245",
publisher = "Schattauer",
number = "2",

}

RIS

TY - JOUR

T1 - Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes

AU - Budde, U

AU - Schneppenheim, R

AU - Plendl, H

AU - Dent, J

AU - Ruggeri, Z M

AU - Zimmerman, T S

PY - 1990/4/12

Y1 - 1990/4/12

N2 - Two methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, IIE, and IIF. Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf:Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf:Ag detectable after 1:300 dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.

AB - Two methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, IIE, and IIF. Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf:Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf:Ag detectable after 1:300 dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.

KW - Autoradiography

KW - Blotting, Western

KW - Collodion

KW - Electrophoresis, Agar Gel

KW - Humans

KW - Luminescent Measurements

KW - Macromolecular Substances

KW - Membranes, Artificial

KW - von Willebrand Factor

M3 - SCORING: Journal article

C2 - 2363131

VL - 63

SP - 312

EP - 315

JO - THROMB HAEMOSTASIS

JF - THROMB HAEMOSTASIS

SN - 0340-6245

IS - 2

ER -