Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes
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Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes. / Budde, U; Schneppenheim, R; Plendl, H; Dent, J; Ruggeri, Z M; Zimmerman, T S.
in: THROMB HAEMOSTASIS, Jahrgang 63, Nr. 2, 12.04.1990, S. 312-5.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes
AU - Budde, U
AU - Schneppenheim, R
AU - Plendl, H
AU - Dent, J
AU - Ruggeri, Z M
AU - Zimmerman, T S
PY - 1990/4/12
Y1 - 1990/4/12
N2 - Two methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, IIE, and IIF. Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf:Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf:Ag detectable after 1:300 dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.
AB - Two methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, IIE, and IIF. Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf:Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf:Ag detectable after 1:300 dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.
KW - Autoradiography
KW - Blotting, Western
KW - Collodion
KW - Electrophoresis, Agar Gel
KW - Humans
KW - Luminescent Measurements
KW - Macromolecular Substances
KW - Membranes, Artificial
KW - von Willebrand Factor
M3 - SCORING: Journal article
C2 - 2363131
VL - 63
SP - 312
EP - 315
JO - THROMB HAEMOSTASIS
JF - THROMB HAEMOSTASIS
SN - 0340-6245
IS - 2
ER -