Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3
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Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3. / Tura, Aysegül; Thieme, Christiane; Brosig, Anton; Merz, Hartmut; Ranjbar, Mahdy; Vardanyan, Siranush; Zuo, Huaxin; Maassen, Tjorge; Kakkassery, Vinodh; Grisanti, Salvatore.
In: INVEST OPHTH VIS SCI, Vol. 61, No. 5, 11.05.2020, p. 12.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3
AU - Tura, Aysegül
AU - Thieme, Christiane
AU - Brosig, Anton
AU - Merz, Hartmut
AU - Ranjbar, Mahdy
AU - Vardanyan, Siranush
AU - Zuo, Huaxin
AU - Maassen, Tjorge
AU - Kakkassery, Vinodh
AU - Grisanti, Salvatore
PY - 2020/5/11
Y1 - 2020/5/11
N2 - PURPOSE: Adiponectin is an insulin-sensitizing and anticarcinogenic hormone that is encoded by a gene on chromosome 3. Here, we analyzed the expression of adiponectin and its receptor Adipor1 in primary uveal melanoma (UM) with regard to the monosomy-3 status and clinical factors, as well as the physiological response of UM cells to adiponectin.METHODS: Immunohistochemistry was performed on the primary UM of 34 patients. Circulating melanoma cells (CMC) were isolated by immunomagnetic enrichment. Monosomy-3 was evaluated by Immuno-FISH. Gene expression was analyzed using the RNAseq data of The Cancer Genome Atlas study. Cultures of choroidal melanocytes and UM were established from the samples of two patients. The proliferative potential of the UM cell lines Mel-270 and OMM-2.5 was determined by immunocytochemistry, immunoblotting, cell cycle analysis, nucleolar staining, and adenosine triphosphate (ATP) levels.RESULTS: UM with monosomy-3 exhibited a lower immunoreactivity for adiponectin and Adipor1, which was associated with monosomy-3-positive CMC and the development of extraocular growth or metastases. Both proteins were more abundant in the irradiated tumors and present in the cultured cells. Gene expression profile indicated the impairment of adiponectin-mediated signaling in the monosomy-3 tumors. Adiponectin induced a significant decline in the ATP levels, Ki-67 expression, cells in the G2/M phase, and nucleolar integrity in UM cultures.CONCLUSIONS: Adiponectin deficiency appears to enhance the metastatic potential of the UM cells with monosomy-3 and the termination of tumor dormancy. Counteracting insulin resistance and improving the serum adiponectin levels might therefore be a valuable approach to prevent or delay the UM metastases.
AB - PURPOSE: Adiponectin is an insulin-sensitizing and anticarcinogenic hormone that is encoded by a gene on chromosome 3. Here, we analyzed the expression of adiponectin and its receptor Adipor1 in primary uveal melanoma (UM) with regard to the monosomy-3 status and clinical factors, as well as the physiological response of UM cells to adiponectin.METHODS: Immunohistochemistry was performed on the primary UM of 34 patients. Circulating melanoma cells (CMC) were isolated by immunomagnetic enrichment. Monosomy-3 was evaluated by Immuno-FISH. Gene expression was analyzed using the RNAseq data of The Cancer Genome Atlas study. Cultures of choroidal melanocytes and UM were established from the samples of two patients. The proliferative potential of the UM cell lines Mel-270 and OMM-2.5 was determined by immunocytochemistry, immunoblotting, cell cycle analysis, nucleolar staining, and adenosine triphosphate (ATP) levels.RESULTS: UM with monosomy-3 exhibited a lower immunoreactivity for adiponectin and Adipor1, which was associated with monosomy-3-positive CMC and the development of extraocular growth or metastases. Both proteins were more abundant in the irradiated tumors and present in the cultured cells. Gene expression profile indicated the impairment of adiponectin-mediated signaling in the monosomy-3 tumors. Adiponectin induced a significant decline in the ATP levels, Ki-67 expression, cells in the G2/M phase, and nucleolar integrity in UM cultures.CONCLUSIONS: Adiponectin deficiency appears to enhance the metastatic potential of the UM cells with monosomy-3 and the termination of tumor dormancy. Counteracting insulin resistance and improving the serum adiponectin levels might therefore be a valuable approach to prevent or delay the UM metastases.
KW - Adenosine Triphosphate/metabolism
KW - Adiponectin/metabolism
KW - Aged
KW - Chromosomes, Human, Pair 3
KW - Female
KW - G2 Phase Cell Cycle Checkpoints
KW - Humans
KW - Immunohistochemistry
KW - Ki-67 Antigen/metabolism
KW - Male
KW - Melanoma/genetics
KW - Monosomy
KW - Neoplasm Metastasis
KW - Neoplastic Cells, Circulating/pathology
KW - Receptors, Adiponectin/metabolism
KW - Uveal Neoplasms/genetics
U2 - 10.1167/iovs.61.5.12
DO - 10.1167/iovs.61.5.12
M3 - SCORING: Journal article
C2 - 32396633
VL - 61
SP - 12
JO - INVEST OPHTH VIS SCI
JF - INVEST OPHTH VIS SCI
SN - 0146-0404
IS - 5
ER -