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Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3

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Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3. / Tura, Aysegül; Thieme, Christiane; Brosig, Anton; Merz, Hartmut; Ranjbar, Mahdy; Vardanyan, Siranush; Zuo, Huaxin; Maassen, Tjorge; Kakkassery, Vinodh; Grisanti, Salvatore.

in: INVEST OPHTH VIS SCI, Jahrgang 61, Nr. 5, 11.05.2020, S. 12.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Tura, A, Thieme, C, Brosig, A, Merz, H, Ranjbar, M, Vardanyan, S, Zuo, H, Maassen, T, Kakkassery, V & Grisanti, S 2020, 'Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3', INVEST OPHTH VIS SCI, Jg. 61, Nr. 5, S. 12. https://doi.org/10.1167/iovs.61.5.12

APA

Tura, A., Thieme, C., Brosig, A., Merz, H., Ranjbar, M., Vardanyan, S., Zuo, H., Maassen, T., Kakkassery, V., & Grisanti, S. (2020). Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3. INVEST OPHTH VIS SCI, 61(5), 12. https://doi.org/10.1167/iovs.61.5.12

Vancouver

Tura A, Thieme C, Brosig A, Merz H, Ranjbar M, Vardanyan S et al. Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3. INVEST OPHTH VIS SCI. 2020 Mai 11;61(5):12. https://doi.org/10.1167/iovs.61.5.12

Bibtex

@article{39ccdd0228314cfcb796c9c9d4cc1c84,
title = "Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3",
abstract = "PURPOSE: Adiponectin is an insulin-sensitizing and anticarcinogenic hormone that is encoded by a gene on chromosome 3. Here, we analyzed the expression of adiponectin and its receptor Adipor1 in primary uveal melanoma (UM) with regard to the monosomy-3 status and clinical factors, as well as the physiological response of UM cells to adiponectin.METHODS: Immunohistochemistry was performed on the primary UM of 34 patients. Circulating melanoma cells (CMC) were isolated by immunomagnetic enrichment. Monosomy-3 was evaluated by Immuno-FISH. Gene expression was analyzed using the RNAseq data of The Cancer Genome Atlas study. Cultures of choroidal melanocytes and UM were established from the samples of two patients. The proliferative potential of the UM cell lines Mel-270 and OMM-2.5 was determined by immunocytochemistry, immunoblotting, cell cycle analysis, nucleolar staining, and adenosine triphosphate (ATP) levels.RESULTS: UM with monosomy-3 exhibited a lower immunoreactivity for adiponectin and Adipor1, which was associated with monosomy-3-positive CMC and the development of extraocular growth or metastases. Both proteins were more abundant in the irradiated tumors and present in the cultured cells. Gene expression profile indicated the impairment of adiponectin-mediated signaling in the monosomy-3 tumors. Adiponectin induced a significant decline in the ATP levels, Ki-67 expression, cells in the G2/M phase, and nucleolar integrity in UM cultures.CONCLUSIONS: Adiponectin deficiency appears to enhance the metastatic potential of the UM cells with monosomy-3 and the termination of tumor dormancy. Counteracting insulin resistance and improving the serum adiponectin levels might therefore be a valuable approach to prevent or delay the UM metastases.",
keywords = "Adenosine Triphosphate/metabolism, Adiponectin/metabolism, Aged, Chromosomes, Human, Pair 3, Female, G2 Phase Cell Cycle Checkpoints, Humans, Immunohistochemistry, Ki-67 Antigen/metabolism, Male, Melanoma/genetics, Monosomy, Neoplasm Metastasis, Neoplastic Cells, Circulating/pathology, Receptors, Adiponectin/metabolism, Uveal Neoplasms/genetics",
author = "Ayseg{\"u}l Tura and Christiane Thieme and Anton Brosig and Hartmut Merz and Mahdy Ranjbar and Siranush Vardanyan and Huaxin Zuo and Tjorge Maassen and Vinodh Kakkassery and Salvatore Grisanti",
year = "2020",
month = may,
day = "11",
doi = "10.1167/iovs.61.5.12",
language = "English",
volume = "61",
pages = "12",
journal = "INVEST OPHTH VIS SCI",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "5",

}

RIS

TY - JOUR

T1 - Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3

AU - Tura, Aysegül

AU - Thieme, Christiane

AU - Brosig, Anton

AU - Merz, Hartmut

AU - Ranjbar, Mahdy

AU - Vardanyan, Siranush

AU - Zuo, Huaxin

AU - Maassen, Tjorge

AU - Kakkassery, Vinodh

AU - Grisanti, Salvatore

PY - 2020/5/11

Y1 - 2020/5/11

N2 - PURPOSE: Adiponectin is an insulin-sensitizing and anticarcinogenic hormone that is encoded by a gene on chromosome 3. Here, we analyzed the expression of adiponectin and its receptor Adipor1 in primary uveal melanoma (UM) with regard to the monosomy-3 status and clinical factors, as well as the physiological response of UM cells to adiponectin.METHODS: Immunohistochemistry was performed on the primary UM of 34 patients. Circulating melanoma cells (CMC) were isolated by immunomagnetic enrichment. Monosomy-3 was evaluated by Immuno-FISH. Gene expression was analyzed using the RNAseq data of The Cancer Genome Atlas study. Cultures of choroidal melanocytes and UM were established from the samples of two patients. The proliferative potential of the UM cell lines Mel-270 and OMM-2.5 was determined by immunocytochemistry, immunoblotting, cell cycle analysis, nucleolar staining, and adenosine triphosphate (ATP) levels.RESULTS: UM with monosomy-3 exhibited a lower immunoreactivity for adiponectin and Adipor1, which was associated with monosomy-3-positive CMC and the development of extraocular growth or metastases. Both proteins were more abundant in the irradiated tumors and present in the cultured cells. Gene expression profile indicated the impairment of adiponectin-mediated signaling in the monosomy-3 tumors. Adiponectin induced a significant decline in the ATP levels, Ki-67 expression, cells in the G2/M phase, and nucleolar integrity in UM cultures.CONCLUSIONS: Adiponectin deficiency appears to enhance the metastatic potential of the UM cells with monosomy-3 and the termination of tumor dormancy. Counteracting insulin resistance and improving the serum adiponectin levels might therefore be a valuable approach to prevent or delay the UM metastases.

AB - PURPOSE: Adiponectin is an insulin-sensitizing and anticarcinogenic hormone that is encoded by a gene on chromosome 3. Here, we analyzed the expression of adiponectin and its receptor Adipor1 in primary uveal melanoma (UM) with regard to the monosomy-3 status and clinical factors, as well as the physiological response of UM cells to adiponectin.METHODS: Immunohistochemistry was performed on the primary UM of 34 patients. Circulating melanoma cells (CMC) were isolated by immunomagnetic enrichment. Monosomy-3 was evaluated by Immuno-FISH. Gene expression was analyzed using the RNAseq data of The Cancer Genome Atlas study. Cultures of choroidal melanocytes and UM were established from the samples of two patients. The proliferative potential of the UM cell lines Mel-270 and OMM-2.5 was determined by immunocytochemistry, immunoblotting, cell cycle analysis, nucleolar staining, and adenosine triphosphate (ATP) levels.RESULTS: UM with monosomy-3 exhibited a lower immunoreactivity for adiponectin and Adipor1, which was associated with monosomy-3-positive CMC and the development of extraocular growth or metastases. Both proteins were more abundant in the irradiated tumors and present in the cultured cells. Gene expression profile indicated the impairment of adiponectin-mediated signaling in the monosomy-3 tumors. Adiponectin induced a significant decline in the ATP levels, Ki-67 expression, cells in the G2/M phase, and nucleolar integrity in UM cultures.CONCLUSIONS: Adiponectin deficiency appears to enhance the metastatic potential of the UM cells with monosomy-3 and the termination of tumor dormancy. Counteracting insulin resistance and improving the serum adiponectin levels might therefore be a valuable approach to prevent or delay the UM metastases.

KW - Adenosine Triphosphate/metabolism

KW - Adiponectin/metabolism

KW - Aged

KW - Chromosomes, Human, Pair 3

KW - Female

KW - G2 Phase Cell Cycle Checkpoints

KW - Humans

KW - Immunohistochemistry

KW - Ki-67 Antigen/metabolism

KW - Male

KW - Melanoma/genetics

KW - Monosomy

KW - Neoplasm Metastasis

KW - Neoplastic Cells, Circulating/pathology

KW - Receptors, Adiponectin/metabolism

KW - Uveal Neoplasms/genetics

U2 - 10.1167/iovs.61.5.12

DO - 10.1167/iovs.61.5.12

M3 - SCORING: Journal article

C2 - 32396633

VL - 61

SP - 12

JO - INVEST OPHTH VIS SCI

JF - INVEST OPHTH VIS SCI

SN - 0146-0404

IS - 5

ER -