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Low molecular thymic peptides stimulate human blood dendritic cells

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Low molecular thymic peptides stimulate human blood dendritic cells. / Mayer, G; Pohlmeyer, K; Caliebe, A; Heimueller, E; Behnke, B; Steimann, G; Lange, Claudia; Beuth, J.

In: ANTICANCER RES, Vol. 20, No. 5A, 2000, p. 2873-83.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mayer, G, Pohlmeyer, K, Caliebe, A, Heimueller, E, Behnke, B, Steimann, G, Lange, C & Beuth, J 2000, 'Low molecular thymic peptides stimulate human blood dendritic cells', ANTICANCER RES, vol. 20, no. 5A, pp. 2873-83.

APA

Mayer, G., Pohlmeyer, K., Caliebe, A., Heimueller, E., Behnke, B., Steimann, G., Lange, C., & Beuth, J. (2000). Low molecular thymic peptides stimulate human blood dendritic cells. ANTICANCER RES, 20(5A), 2873-83.

Vancouver

Mayer G, Pohlmeyer K, Caliebe A, Heimueller E, Behnke B, Steimann G et al. Low molecular thymic peptides stimulate human blood dendritic cells. ANTICANCER RES. 2000;20(5A):2873-83.

Bibtex

@article{2ffed1f2a39b4d4094893dcc038a8eb9,
title = "Low molecular thymic peptides stimulate human blood dendritic cells",
abstract = "Dendritic cells are considered to be the most potent antigen-presenting cells and are thus promising new tools for the immunotherapy of cancer. They respond to various stimuli by differentiation (expression of CD83) and up-regulation of costimulatory surface molecules. Thymic peptides have immunostimulatory and immunomodulating properties. Their therapeutic potential in immunotherapy of cancer has been discussed. To test whether thymic peptides act on dendritic cells, we examined the effects of a standardized thymic peptide preparation on cultured human monocyte-derived dendritic cells. Addition of thymic peptides resulted in enhanced expression of the specific differentiation marker CD83 in a dose dependent manner. Moreover, thymic peptides induced the up-regulation of costimulatory molecules including CD86, CD80, HLA-DR and HLA-ABC. After priming with thymic peptides dendritic cells showed an enhanced expression of IL-8 and TNF-alpha mRNA and protein release. Dendritic cells stimulated with thymic peptides were able to induce proliferation of autologous T cells as measured by 3H-thymidine incorporation in mixed Lymphocyte reaction. In combination with a low dosage of keyhole limpet hemocyanin, thymic peptides showed additive effects in the up-regulation of CD83 and costimulatory surface markers. Our findings indicate that thymic peptides per se act on professional antigen-presenting cells in a stimulatory manner and were presented by these cells. Furthermore, thymic peptides enhance the response of dendritic cells to low dosages of a standard nominal antigen. Therefore, thymic peptides could improve the immunological activity especially against low amounts of endogenous antigens.",
keywords = "Amino Acid Sequence, Animals, Antigens, CD, Antigens, CD40, Antigens, CD80, Antigens, CD86, Cattle, Cell Differentiation, Cell Division, Cells, Cultured, Dendritic Cells, Gene Expression Regulation, HLA-DR Antigens, Haptens, Hemocyanin, Humans, Interleukin-8, Membrane Glycoproteins, Molecular Sequence Data, Molecular Weight, Peptides, RNA, Messenger, T-Lymphocytes, Thymus Gland, Tumor Necrosis Factor-alpha, Up-Regulation",
author = "G Mayer and K Pohlmeyer and A Caliebe and E Heimueller and B Behnke and G Steimann and Claudia Lange and J Beuth",
year = "2000",
language = "English",
volume = "20",
pages = "2873--83",
journal = "ANTICANCER RES",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "5A",

}

RIS

TY - JOUR

T1 - Low molecular thymic peptides stimulate human blood dendritic cells

AU - Mayer, G

AU - Pohlmeyer, K

AU - Caliebe, A

AU - Heimueller, E

AU - Behnke, B

AU - Steimann, G

AU - Lange, Claudia

AU - Beuth, J

PY - 2000

Y1 - 2000

N2 - Dendritic cells are considered to be the most potent antigen-presenting cells and are thus promising new tools for the immunotherapy of cancer. They respond to various stimuli by differentiation (expression of CD83) and up-regulation of costimulatory surface molecules. Thymic peptides have immunostimulatory and immunomodulating properties. Their therapeutic potential in immunotherapy of cancer has been discussed. To test whether thymic peptides act on dendritic cells, we examined the effects of a standardized thymic peptide preparation on cultured human monocyte-derived dendritic cells. Addition of thymic peptides resulted in enhanced expression of the specific differentiation marker CD83 in a dose dependent manner. Moreover, thymic peptides induced the up-regulation of costimulatory molecules including CD86, CD80, HLA-DR and HLA-ABC. After priming with thymic peptides dendritic cells showed an enhanced expression of IL-8 and TNF-alpha mRNA and protein release. Dendritic cells stimulated with thymic peptides were able to induce proliferation of autologous T cells as measured by 3H-thymidine incorporation in mixed Lymphocyte reaction. In combination with a low dosage of keyhole limpet hemocyanin, thymic peptides showed additive effects in the up-regulation of CD83 and costimulatory surface markers. Our findings indicate that thymic peptides per se act on professional antigen-presenting cells in a stimulatory manner and were presented by these cells. Furthermore, thymic peptides enhance the response of dendritic cells to low dosages of a standard nominal antigen. Therefore, thymic peptides could improve the immunological activity especially against low amounts of endogenous antigens.

AB - Dendritic cells are considered to be the most potent antigen-presenting cells and are thus promising new tools for the immunotherapy of cancer. They respond to various stimuli by differentiation (expression of CD83) and up-regulation of costimulatory surface molecules. Thymic peptides have immunostimulatory and immunomodulating properties. Their therapeutic potential in immunotherapy of cancer has been discussed. To test whether thymic peptides act on dendritic cells, we examined the effects of a standardized thymic peptide preparation on cultured human monocyte-derived dendritic cells. Addition of thymic peptides resulted in enhanced expression of the specific differentiation marker CD83 in a dose dependent manner. Moreover, thymic peptides induced the up-regulation of costimulatory molecules including CD86, CD80, HLA-DR and HLA-ABC. After priming with thymic peptides dendritic cells showed an enhanced expression of IL-8 and TNF-alpha mRNA and protein release. Dendritic cells stimulated with thymic peptides were able to induce proliferation of autologous T cells as measured by 3H-thymidine incorporation in mixed Lymphocyte reaction. In combination with a low dosage of keyhole limpet hemocyanin, thymic peptides showed additive effects in the up-regulation of CD83 and costimulatory surface markers. Our findings indicate that thymic peptides per se act on professional antigen-presenting cells in a stimulatory manner and were presented by these cells. Furthermore, thymic peptides enhance the response of dendritic cells to low dosages of a standard nominal antigen. Therefore, thymic peptides could improve the immunological activity especially against low amounts of endogenous antigens.

KW - Amino Acid Sequence

KW - Animals

KW - Antigens, CD

KW - Antigens, CD40

KW - Antigens, CD80

KW - Antigens, CD86

KW - Cattle

KW - Cell Differentiation

KW - Cell Division

KW - Cells, Cultured

KW - Dendritic Cells

KW - Gene Expression Regulation

KW - HLA-DR Antigens

KW - Haptens

KW - Hemocyanin

KW - Humans

KW - Interleukin-8

KW - Membrane Glycoproteins

KW - Molecular Sequence Data

KW - Molecular Weight

KW - Peptides

KW - RNA, Messenger

KW - T-Lymphocytes

KW - Thymus Gland

KW - Tumor Necrosis Factor-alpha

KW - Up-Regulation

M3 - SCORING: Journal article

C2 - 11062696

VL - 20

SP - 2873

EP - 2883

JO - ANTICANCER RES

JF - ANTICANCER RES

SN - 0250-7005

IS - 5A

ER -